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Recombinant Human CDNF Protein Standard (His tag) is a Human Full Length protein, expressed in HEK 293, with >=80% purity and suitable for sELISA, SDS-PAGE.

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Images

SDS-PAGE - Recombinant Human CDNF Protein Standard (His tag) (AB323824), expandable thumbnail
  • Sandwich ELISA - Recombinant Human CDNF Protein Standard (His tag) (AB323824), expandable thumbnail

Key facts

Purity
>=80% SDS-PAGE
Expression system
HEK 293 cells
Tags
His tag C-Terminus
Applications
sELISA, SDS-PAGE
Biologically active
No

Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Target data

Function

Trophic factor for dopamine neurons. Prevents the 6-hydroxydopamine (6-OHDA)-induced degeneration of dopaminergic neurons. When administered after 6-OHDA-lesioning, restores the dopaminergic function and prevents the degeneration of dopaminergic neurons in substantia nigra (By similarity).

Alternative names

Recombinant Human CDNF Protein Standard (His tag) is a Human Full Length protein, expressed in HEK 293, with >=80% purity and suitable for sELISA, SDS-PAGE.

Key facts

Purity
>=80% SDS-PAGE
Expression system
HEK 293 cells
Applications
sELISA, SDS-PAGE
Accession
Q49AH0-1
Animal free
Yes
Species
Human
Concentration
Loading...
Storage buffer

pH: 7.3 - 7.5
Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic

Sequence info

Amino acid sequence

Accession
Q49AH0
Protein length
Full Length
Predicted molecular weight
20 kDa
Nature
Recombinant
Tags
His tag C-Terminus

Specifications

Form
Liquid

General info

Function

Trophic factor for dopamine neurons. Prevents the 6-hydroxydopamine (6-OHDA)-induced degeneration of dopaminergic neurons. When administered after 6-OHDA-lesioning, restores the dopaminergic function and prevents the degeneration of dopaminergic neurons in substantia nigra (By similarity).

Sequence similarities

Belongs to the ARMET family.

Storage

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle

Notes

While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.

Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.

The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • SDS-PAGE - Recombinant Human CDNF Protein Standard (His tag) (ab323824), expandable thumbnail

    SDS-PAGE - Recombinant Human CDNF Protein Standard (His tag) (ab323824)

    SDS-PAGE analysis of ab323824 under reducing conditions for 2ug protein.

  • Sandwich ELISA - Recombinant Human CDNF Protein Standard (His tag) (ab323824), expandable thumbnail

    Sandwich ELISA - Recombinant Human CDNF Protein Standard (His tag) (ab323824)

    Sandwich ELISA with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com