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AB114247

Recombinant Human CDX2 protein (GST tag N-Terminus)

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Recombinant Human CDX2 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 313 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

CDX3, CDX2, Homeobox protein CDX-2, CDX-3, Caudal-type homeobox protein 2

2 Images
Western blot - Recombinant Human CDX2 protein (GST tag N-Terminus) (AB114247)
  • WB

Unknown

Western blot - Recombinant Human CDX2 protein (GST tag N-Terminus) (AB114247)

ab88129 recognizes the full length tagged recombinant CDX2 protein (ab114247) which has an expected molecular weight of 61 kDa.

All lanes:

Anti-CDX2 antibody (<a href='/en-us/products/unavailable/cdx2-antibody-ab88129'>ab88129</a>) at 1 µg/mL

All lanes:

Western blot - Recombinant Human CDX2 protein (GST tag N-Terminus) (ab114247) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

true

Exposure time: 10s

SDS-PAGE - Recombinant Human CDX2 protein (GST tag N-Terminus) (AB114247)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human CDX2 protein (GST tag N-Terminus) (AB114247)

12.5% SDS-PAGE showing ab114247 at approximately 60.54kDa stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, SDS-PAGE, ELISA

applications

Biologically active

No

Accession

Q99626

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.3% Glutathione

storage-buffer

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Complementary Products

Primary Antibodies

AB76541

Anti-CDX2 antibody [EPR2764Y]

RabMAb|Recombinant|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [EPR2764Y] (AB76541)

  • 4
  • 17
Primary Antibodies

AB181598

Anti-Cytokeratin 7 antibody [EPR17078] - Cytoskeleton Marker

KO Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 7 antibody [EPR17078] - Cytoskeleton Marker (AB181598)

  • 5
  • 13
Primary Antibodies

AB97959

Anti-SOX2 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody (AB97959)

  • 4
  • 76
Primary Antibodies

AB185966

Anti-SOX9 antibody [EPR14335-78]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] (AB185966)

  • 5
  • 30
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab114247 can be used as a WB positive control in conjunction with <a href='/en-us/products/unavailable/cdx2-antibody-ab88129'>ab88129</a>.</p>" } } }
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Sequence info

[{"sequence":"MYVSYLLDKDVSMYPSSVRHSGGLNLAPQNFVSPPQYPDYGGYHVAAAAAAAANLDSAQSPGPSWPAAYGAPLREDWNGYAPGGAAAAANAVAHGPNGGSPAAAMGYSSPADYHPHHHPHHHPHHPAAAPSCASGLLQTLNPGPPGPAATAAAEQLSPGGQRRNLCEWMRKPAQQSLGSQVKTRTKDKYRVVYTDHQRLELEKEFHYSRYITIRRKAELAATLGLSERQVKIWFQNRRAKERKINKKKLQQQQQQQPPQPPPPPPQPPQPQPGPLRSVPEPLSPVSSLQASVSGSVPGVLGPTGGVLNPTVTQ","proteinLength":"Full Length","predictedMolecularWeight":"60.54 kDa","actualMolecularWeight":null,"aminoAcidEnd":313,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q99626","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CDX2 or Caudal Type Homeobox 2 is a transcription factor involved in intestinal development and differentiation. Alternative names for CDX2 include Cdx-2 and CDX-2. This protein has a molecular mass of approximately 38 kDa. CDX2 is expressed mainly in the epithelial cells of the intestine. Researchers can identify CDX2 through techniques like CDX2 stain CDX2 immunostain and CDX2 IHC which are useful for determining its expression in tissue samples.
Biological function summary

CDX2 plays an important role in controlling the expression of genes necessary for maintaining intestinal epithelial cell identity. It functions as a part of regulatory networks that guide cell fate and differentiation in the gut. CDX2 does not generally form complexes but acts as a transcriptional regulator that binds directly to DNA influencing genes involved in cell adhesion proliferation and the maintenance of tissue structure.

Pathways

CDX2 is integral to the Wnt signaling and Hedgehog pathways which are vital for intestinal development and homeostasis. Within these pathways CDX2 works closely with other proteins like beta-catenin influencing cell proliferation and differentiation. The Wnt pathway in particular modulates the expression of proliferative genes while CDX2 ensures that proliferation maintains tissue integrity by guiding cellular differentiation.

CDX2 has a significant link to colorectal cancer and Barrett's esophagus. Loss of CDX2 expression often marks the progression from normal to malignant tissue in colorectal cancer where it connects with proteins like p-catenin affecting cellular growth and differentiation. Similarly abnormal expression of CDX2 correlates with the development of Barrett's esophagus a condition that may progress to esophageal adenocarcinoma. These disease associations make CDX2 a potential diagnostic marker and therapeutic target in gastrointestinal pathologies.
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Specifications

Form

Liquid

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General info

Function

Transcription factor which regulates the transcription of multiple genes expressed in the intestinal epithelium (By similarity). Binds to the promoter of the intestinal sucrase-isomaltase SI and activates SI transcription (By similarity). Binds to the DNA sequence 5'-ATAAAAACTTAT-3' in the promoter region of VDR and activates VDR transcription (By similarity). Binds to and activates transcription of LPH (By similarity). Activates transcription of CLDN2 and intestinal mucin MUC2 (By similarity). Binds to the 5'-AATTTTTTACAACACCT-3' DNA sequence in the promoter region of CA1 and activates CA1 transcription (By similarity). Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine. Binds preferentially to methylated DNA (PubMed : 28473536).

Sequence similarities

Belongs to the Caudal homeobox family.

Post-translational modifications

Ubiquitinated, leading to its degradation by the proteasome.. Phosphorylation at Ser-60 reduces transactivation capacity. Phosphorylation at Ser-283 reduces transactivation capacity and also increases ubiquitin-dependent proteasome degradation.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Transcription factor which regulates the transcription of multiple genes expressed in the intestinal epithelium (By similarity). Binds to the promoter of the intestinal sucrase-isomaltase SI and activates SI transcription (By similarity). Binds to the DNA sequence 5'-ATAAAAACTTAT-3' in the promoter region of VDR and activates VDR transcription (By similarity). Binds to and activates transcription of LPH (By similarity). Activates transcription of CLDN2 and intestinal mucin MUC2 (By similarity). Binds to the 5'-AATTTTTTACAACACCT-3' DNA sequence in the promoter region of CA1 and activates CA1 transcription (By similarity). Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine. Binds preferentially to methylated DNA (PubMed : 28473536).
See full target information CDX2
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