Recombinant Human CEACAM5/CEA Protein (His-tag) is a Human Full Length protein, expressed in HEK 293, with >=95% purity, <= 1.9 EU/mg endotoxin level and suitable for HPLC, MS, SDS-PAGE.
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Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 1.9 EU/mg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- HPLC, MS, SDS-PAGE
- Biologically active
- No
- Mass spectrometry
- LC-MS/MS
Amino acid sequence
K L T I E S T P F N V A E G K E V L L L V H N L P Q H L F G Y S W Y K G E R V D G N R Q I I G Y V I G T Q Q A T P G P A Y S G R E I I Y P N A S L L I Q N I I Q N D T G F Y T L H V I K S D L V N E E A T G Q F R V Y P E L P K P S I S S N N S K P V E D K D A V A F T C E P E T Q D A T Y L W W V N N Q S L P V S P R L Q L S N G N R T L T L F N V T R N D T A S Y K C E T Q N P V S A R R S D S V I L N V L Y G P D A P T I S P L N T S Y R S G E N L N L S C H A A S N P P A Q Y S W F V N G T F Q Q S T Q E L F I P N I T V N N S G S Y T C Q A H N S D T G L N R T T V T T I T V Y A E P P K P F I T S N N S N P V E D E D A V A L T C E P E I Q N T T Y L W W V N N Q S L P V S P R L Q L S N D N R T L T L L S V T R N D V G P Y E C G I Q N E L S V D H S D P V I L N V L Y G P D D P T I S P S Y T Y Y R P G V N L S L S C H A A S N P P A Q Y S W L I D G N I Q Q H T Q E L F I S N I T E K N S G L Y T C Q A N N S A S G H S R T T V K T I T V S A E L P K P S I S S N N S K P V E D K D A V A F T C E P E A Q N T T Y L W W V N G Q S L P V S P R L Q L S N G N R T L T L F N V T R N D A R A Y V C G I Q N S V S A N R S D P V T L D V L Y G P D T P I I S P P D S S Y L S G A N L N L S C H S A S N P S P Q Y S W R I N G I P Q Q H T Q V L F I A K I T P N N N G T Y A C F V S N L A T G R N N S I V K S I T V S A S G T S P G L S A
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Cell surface glycoprotein that plays a role in cell adhesion, intracellular signaling and tumor progression (PubMed:10864933, PubMed:10910050, PubMed:2803308). Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6 (PubMed:2803308). Plays a role as an oncogene by promoting tumor progression; induces resistance to anoikis of colorectal carcinoma cells (PubMed:10910050). (Microbial infection) Receptor for E.coli Dr adhesins. Binding of E.coli Dr adhesins leads to dissociation of the homodimer.
Alternative names
CD66e, CEA, CEACAM5, Cell adhesion molecule CEACAM5, Carcinoembryonic antigen, Carcinoembryonic antigen-related cell adhesion molecule 5, Meconium antigen 100, CEA cell adhesion molecule 5
Recommended products
Recombinant Human CEACAM5/CEA Protein (His-tag) is a Human Full Length protein, expressed in HEK 293, with >=95% purity, <= 1.9 EU/mg endotoxin level and suitable for HPLC, MS, SDS-PAGE.
Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 1.9 EU/mg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- HPLC, MS, SDS-PAGE
- Biologically active
- No
- Mass spectrometry
- LC-MS/MS
- Accession
- P06731-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- K L T I E S T P F N V A E G K E V L L L V H N L P Q H L F G Y S W Y K G E R V D G N R Q I I G Y V I G T Q Q A T P G P A Y S G R E I I Y P N A S L L I Q N I I Q N D T G F Y T L H V I K S D L V N E E A T G Q F R V Y P E L P K P S I S S N N S K P V E D K D A V A F T C E P E T Q D A T Y L W W V N N Q S L P V S P R L Q L S N G N R T L T L F N V T R N D T A S Y K C E T Q N P V S A R R S D S V I L N V L Y G P D A P T I S P L N T S Y R S G E N L N L S C H A A S N P P A Q Y S W F V N G T F Q Q S T Q E L F I P N I T V N N S G S Y T C Q A H N S D T G L N R T T V T T I T V Y A E P P K P F I T S N N S N P V E D E D A V A L T C E P E I Q N T T Y L W W V N N Q S L P V S P R L Q L S N D N R T L T L L S V T R N D V G P Y E C G I Q N E L S V D H S D P V I L N V L Y G P D D P T I S P S Y T Y Y R P G V N L S L S C H A A S N P P A Q Y S W L I D G N I Q Q H T Q E L F I S N I T E K N S G L Y T C Q A N N S A S G H S R T T V K T I T V S A E L P K P S I S S N N S K P V E D K D A V A F T C E P E A Q N T T Y L W W V N G Q S L P V S P R L Q L S N G N R T L T L F N V T R N D A R A Y V C G I Q N S V S A N R S D P V T L D V L Y G P D T P I I S P P D S S Y L S G A N L N L S C H S A S N P S P Q Y S W R I N G I P Q Q H T Q V L F I A K I T P N N N G T Y A C F V S N L A T G R N N S I V K S I T V S A S G T S P G L S A
- Accession
- P06731
- Protein length
- Full Length
- Predicted molecular weight
- 72.43 kDa
- Actual molecular weight
- 72.45 kDa
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Cell surface glycoprotein that plays a role in cell adhesion, intracellular signaling and tumor progression (PubMed:10864933, PubMed:10910050, PubMed:2803308). Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6 (PubMed:2803308). Plays a role as an oncogene by promoting tumor progression; induces resistance to anoikis of colorectal carcinoma cells (PubMed:10910050).
- Sequence similarities
Belongs to the immunoglobulin superfamily. CEA family.
- Post-translational modifications
Complex immunoreactive glycoprotein with a MW of 180 kDa comprising 60% carbohydrate.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CEACAM5 also known as Carcinoembryonic Antigen (CEA) or CD66e is a glycoprotein found mainly on the surface of epithelial cells. This protein has a mass of approximately 180 kDa. It gets expressed in a variety of tissues with high expression in the gastrointestinal tract including the colon and rectum. CEACAM5 is a member of the immunoglobulin superfamily and plays a role in cell adhesion influencing how cells stick to each other.
- Biological function summary
CEACAM5 contributes to cellular processes involving cell adhesion and immune response modulation. This protein operates as part of a larger group of cell adhesion molecules and does not function as part of a complex. CEACAM5 can affect the interactions between cells and the extracellular matrix which has implications for tissue structuring and integrity. It helps the immune system recognize and respond to infectious agents or cellular anomalies.
- Pathways
CEACAM5 involvement extends to immune response regulation and cell adhesion pathways. It interacts functionally with proteins like E-cadherin in these pathways which contribute to maintaining epithelial integrity. These pathways are critical for normal tissue maintenance and immune system function especially in mucosal surfaces where pathogen entry occurs. Understanding CEACAM5 interactions provides insight into cellular communication and adhesion mechanisms.
- Associated diseases and disorders
CEACAM5 is associated with colorectal cancer and lung adenocarcinoma. Its overexpression and release into the bloodstream make it a popular biomarker for these diseases. CEACAM5 is used in clinical applications such as CEA ELISA assays for cancer detection and monitoring. In colorectal cancer CEACAM5 interacts with proteins like CEACAM6 to promote tumor invasiveness and metastasis. Anti-CEACAM5 therapeutics including antibody-drug conjugates (anti-CEACAM5 ADC) are being developed to target and treat associated malignancies.
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3 product images
SDS-PAGE - Recombinant Human CEACAM5/CEA Protein (His-tag) (ab317870)
SDS-PAGE analysis of ab317870 under reducing conditions for 2ug protein.
Mass Spectrometry - Recombinant Human CEACAM5/CEA Protein (His-tag) (ab317870)
Mass determination by ESI-TOF. Predicted MW is 72430 Da (+/- 10 Da by ESI-TOF). Observed MW is 72452.54 Da.
HPLC - Recombinant Human CEACAM5/CEA Protein (His-tag) (ab317870)
HPLC analysis of ab317870
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