Recombinant Human CENPB protein (His tag)

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CENP-B also known as Centromere Protein B is a protein with a molecular weight of approximately 80 kDa. It binds to the centromeric DNA and plays a critical role in centromere formation and function. CENP-B is heavily expressed in dividing cells particularly in tissues where cell proliferation is high such as bone marrow and epithelial tissues. Researchers often use tools like anti-CENP-B antibodies and CENP-B ELISA kits to study its expression in various experimental settings.

Biological function summary

CENP-B participates in the assembly of the centromere protein complex contributing to the stability and maintenance of the centromere structure during cell division. It acts as a foundation for kinetochore nucleation ensuring proper chromosome segregation. CENP-B is involved in the formation of the CENP-A nucleosome complex important for accurate chromosomal alignment and separation.

Pathways

CENP-B is essential in the mitotic spindle assembly and chromosome segregation pathways. This protein interacts with CENP-A a variant histone which integrates into centromeric nucleosomes to initiate the formation of functional kinetochores. Additionally CENP-B interacts with CENP-C facilitating the recruitment of other centromere-associated proteins needed for chromosome dynamics during mitosis.

CENP-B is primarily associated with autoimmune disorders such as systemic sclerosis (scleroderma). Patients with these conditions often produce anti-CENP-B antibodies which serve as diagnostic markers. Moreover CENP-B's dysregulation links to chromosomal instability a contributor to cancer. Research has shown connections between CENP-B and related proteins like CENP-A in the context of chromosomal aneuploidies and malignancies.