JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB174427

Recombinant Human CENPQ protein (denatured) (His tag N-Terminus)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human CENPQ protein (denatured) (His tag N-Terminus) is a Human Full Length protein, in the 1 to 268 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

C6orf139, CENPQ, Centromere protein Q, CENP-Q

1 Images
SDS-PAGE - Recombinant Human CENPQ protein (denatured) (His tag N-Terminus) (AB174427)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human CENPQ protein (denatured) (His tag N-Terminus) (AB174427)

SDS-PAGE analysis of ab174427 on 15% SDS-PAGE gel (3μg).

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q7L2Z9

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.32% Tris HCl

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMSGKANASKKNAQQLKRNPKRKKDNEEVVLSENKVRNTVKKNKNHLKDLSSEGQTKHTNLKHGKTAASKRKTWQPLSKSTRDHLQTMMESVIMTILSNSIKEKEEIQYHLNFLKKRLLQQCETLKVPPKKMEDLTNVSSLLNMERARDKANEEGLALLQEEIDKMVETTELMTGNIQSLKNKIQILASEVEEEEERVKQMHQINSSGVLSLPELSQKTLKAPTLQKEILALIPNQNALLKDLDILHNSSQMKSMSTFIEEAYKKLDAS","proteinLength":"Full Length","predictedMolecularWeight":"33 kDa","actualMolecularWeight":null,"aminoAcidEnd":268,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q7L2Z9","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CENPQ also known as Centromere Protein Q is a component of the inner kinetochore plate. This protein has an approximate molecular mass of 25 kDa. CENPQ is expressed in various tissues. It localizes at the centromere during cell division. The presence of CENPQ ensures the proper attachment of microtubules to the kinetochore from spindle fibers critical for chromosome alignment and segregation.
Biological function summary

Centromere Protein Q plays a role in the maintenance of centromere structure and function. It forms part of the CENP-H/I/K complex which is involved in assembling the kinetochore. This complex stabilizes the kinetochore-microtubule interactions facilitating the accurate transmission of chromosomes during mitosis. The precise coordination of these activities helps prevent chromosomal instability which could lead to genetic disorders.

Pathways

CENPQ participates in the mitotic spindle checkpoint pathway. This pathway ensures that cells do not progress to anaphase until all chromosomes are properly aligned at the metaphase plate preventing aneuploidy. In addition to this CENPQ interacts with other proteins such as CENP-C and CENP-E. These interactions are essential to kinetochore function and the overall fidelity of cell division.

CENPQ plays a role in cancer and chromosomal instability syndromes. Malfunction or mutations in CENPQ can compromise its ability to maintain proper chromosome segregation which is associated with various forms of cancer. CENPQ's interactions with other centromere proteins like CENP-E are critical for maintaining genomic stability their disruption can lead to tumorigenesis or progression of chromosomal aberrations.
style
left-column

Specifications

Form

Liquid

Additional notes

ab174427 is purified by using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.

style
left-column

General info

Function

Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex (PubMed : 16622420). Plays an important role in chromosome congression and in the recruitment of CENP-O complex (which comprises CENPO, CENPP, CENPQ and CENPU), CENPE and PLK1 to the kinetochores (PubMed : 25395579).

Sequence similarities

Belongs to the CENP-Q/OKP1 family.

Post-translational modifications

Phosphorylation at Ser-50 is essential for CENPE recruitment to kinetochores and orderly chromosome congression.

Subcellular localisation

Nucleus

style
left-column

Product protocols

style
left-column

Target data

Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex (PubMed : 16622420). Plays an important role in chromosome congression and in the recruitment of CENP-O complex (which comprises CENPO, CENPP, CENPQ and CENPU), CENPE and PLK1 to the kinetochores (PubMed : 25395579).
See full target information CENPQ
style
left-column
style
left-column
style
right-column

Complementary Products

Proteins & Peptides

AB177673

Recombinant Human PCDHGC4 protein (denatured) (His tag N-Terminus)

SDS-PAGE - Recombinant Human PCDHGC4 protein (denatured) (His tag N-Terminus) (AB177673)

  • 0
  • 0
Proteins & Peptides

AB174560

Recombinant Human KCTD4 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human KCTD4 protein (His tag N-Terminus) (AB174560)

  • 0
  • 0
Proteins & Peptides

AB174553

Recombinant Human JMJD7 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human JMJD7 protein (His tag N-Terminus) (AB174553)

  • 0
  • 0
Proteins & Peptides

AB174454

Recombinant Human CEND1 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human CEND1 protein (His tag N-Terminus) (AB174454)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com