Recombinant Human CERT protein is a Human Fragment protein, in the 347 to 598 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
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- Chemistry (Weinheim an der Bergstrasse, Germany) 22:6676-862016Asymmetric Synthesis and Binding Study of New Long-Chain HPA-12 Analogues as Potent Ligands of the Ceramide Transfer Protein CERT.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndrej Ďuriš,Adam Daïch,Cécile Santos,Laurence Fleury,Frédéric Ausseil,Frédéric Rodriguez,Stéphanie Ballereau,Yves Génisson,Dušan BerkešPubMed 27031925
- Journal of biomolecular screening 20:779-872015Development of a CERT START Domain-Ceramide HTRF Binding Assay and Application to Pharmacological Studies and Screening.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaurence Fleury et. al.PubMed 25716975
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M R G S H H H H H H G M A S M T G G Q Q M G R D L Y D D D D K D R W A G S M L H W P T S L P S G D A F S S V G T H R F V Q K V E E M V Q N H M T Y S L Q D V G G D A N W Q L V V E E G E M K V Y R R E V E E N G I V L D P L K A T H A V K G V T G H E V C N Y F W N V D V R N D W E T T I E N F H V V E T L A D N A I I I Y Q T H K R V W P A S Q R D V L Y L S V I R K I P A L T E N D P E T W I V C N F S V D H D S A P L N N R C V R A K I N V A M I C Q T L V S P P E G N Q E I S R D N I L C K I T Y V A N V N P G G W A P A S V L R A V A K R E Y P K F L K R F T S Y V Q E K T A G K P I L F
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Shelters ceramides and diacylglycerol lipids inside its START domain and mediates the intracellular trafficking of ceramides and diacylglycerol lipids in a non-vesicular manner.
Alternative names
CERT, COL4A3BP, STARD11, CERT1, Ceramide transfer protein, hCERT, Collagen type IV alpha-3-binding protein, Goodpasture antigen-binding protein, START domain-containing protein 11, StAR-related lipid transfer protein 11, GPBP, StARD11
Recommended products
Recombinant Human CERT protein is a Human Fragment protein, in the 347 to 598 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q9Y5P4-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl
Sequence info
Amino acid sequence
- M R G S H H H H H H G M A S M T G G Q Q M G R D L Y D D D D K D R W A G S M L H W P T S L P S G D A F S S V G T H R F V Q K V E E M V Q N H M T Y S L Q D V G G D A N W Q L V V E E G E M K V Y R R E V E E N G I V L D P L K A T H A V K G V T G H E V C N Y F W N V D V R N D W E T T I E N F H V V E T L A D N A I I I Y Q T H K R V W P A S Q R D V L Y L S V I R K I P A L T E N D P E T W I V C N F S V D H D S A P L N N R C V R A K I N V A M I C Q T L V S P P E G N Q E I S R D N I L C K I T Y V A N V N P G G W A P A S V L R A V A K R E Y P K F L K R F T S Y V Q E K T A G K P I L F
- Accession
- Q9Y5P4
- Protein length
- Fragment
- Predicted molecular weight
- 33.1 kDa
- Amino acids
- 347 to 598
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab95897 is purified using conventional chromatography techniques.
General info
- Function
Shelters ceramides and diacylglycerol lipids inside its START domain and mediates the intracellular trafficking of ceramides and diacylglycerol lipids in a non-vesicular manner.
- Post-translational modifications
Phosphorylation on Ser-132 decreases the affinity toward phosphatidylinositol 4-phosphate at Golgi membranes and reduces ceramide transfer activity. Inactivated by hyperphosphorylation of serine residues by CSNK1G2/CK1 that triggers dissociation from the Golgi complex, thus down-regulating ER-to-Golgi transport of ceramide and sphingomyelin synthesis.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Previously labelled as COL4A3BP.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CERT also known as CERT protein or COL4A3BP functions in the intracellular transport of ceramide from the endoplasmic reticulum to the Golgi apparatus. CERT consists of a PH domain a middle domain and a START domain comprising a mass of approximately 68 kDa. It is expressed in various human tissues with notable expression in the brain and liver. CERT facilitates the synthesis of sphingomyelin an essential component of cellular membranes.
- Biological function summary
CERT plays a central role in lipid metabolism and is part of the ceramide transfer complex. This complex regulates the balance of ceramide and sphingomyelin as these lipids influence cell signaling and apoptosis. The effective transfer of ceramide by CERT ensures proper cell function and integrity. Aberrations in CERT activity can disrupt lipid homeostasis impacting cell survival and signaling pathways.
- Pathways
CERT is integrated into the sphingolipid and lipid signaling pathways. It interacts with proteins like protein kinase D which regulates its phosphorylation status and as a result its activity. In these pathways CERT's role is important for maintaining lipid composition within cell membranes ensuring the proper functioning of signaling processes that depend on lipid-mediated communication.
- Associated diseases and disorders
Disrupted CERT function associates with conditions like cancer and neurodegenerative disorders. In cancer altered ceramide levels due to CERT dysfunction can influence cell growth and survival contributing to tumor progression. For neurodegenerative disorders CERT's interaction with proteins like protein kinase C highlights its significance in maintaining neuronal cell stability and signaling disruption of which may relate to disease development.
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1 product image
SDS-PAGE - Recombinant Human CERT protein (ab95897)
15% SDS-PAGE showing ab95897 at approximately 33.1kDa (3μg).
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