Recombinant Human CGRP Protein (GST tag) is a Human Full Length protein, expressed in Escherichia coli, with >=95% purity, = 0.005 EU/µg endotoxin level and suitable for MS, SDS-PAGE.
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Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- = 0.005 EU/µg
- Expression system
- Escherichia coli
- Tags
- GST tag N-Terminus
- Applications
- MS, SDS-PAGE
- Biologically active
- No
- Mass spectrometry
- LC-MS/MS
Amino acid sequence
A C D T A T C V T H R L A G L L S R S G G V V K N N F V P T N V G S K A
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
CGRP1/CALCA is a peptide hormone that induces vasodilation mediated by the CALCRL-RAMP1 receptor complex (PubMed:1318039, PubMed:33602864, PubMed:9620797). Dilates a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role (PubMed:3492492). It also elevates platelet cAMP (PubMed:1318039). CGRP1 can also bind and activate CALCR-RAMP1 (AMYR1) receptor complex (PubMed:38603770).
Alternative names
CALC1, CALCA, Calcitonin gene-related peptide 1, CGRP1, Alpha-type CGRP, Calcitonin gene-related peptide I, CGRP-I
Recommended products
Recombinant Human CGRP Protein (GST tag) is a Human Full Length protein, expressed in Escherichia coli, with >=95% purity, = 0.005 EU/µg endotoxin level and suitable for MS, SDS-PAGE.
Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- = 0.005 EU/µg
- Expression system
- Escherichia coli
- Tags
- GST tag N-Terminus
- Applications
- MS, SDS-PAGE
- Biologically active
- No
- Mass spectrometry
- LC-MS/MS
- Accession
- P06881-1
- Animal free
- Yes
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 50% Glycerol (glycerin, glycerine), 0.3752% Tris HCl, 0.2431% Sodium chloride
Sequence info
Amino acid sequence
- A C D T A T C V T H R L A G L L S R S G G V V K N N F V P T N V G S K A
- Accession
- P06881
- Protein length
- Full Length
- Predicted molecular weight
- 29.13 kDa
- Actual molecular weight
- 29.13 kDa
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
CGRP1/CALCA is a peptide hormone that induces vasodilation mediated by the CALCRL-RAMP1 receptor complex (PubMed:1318039, PubMed:33602864, PubMed:9620797). Dilates a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role (PubMed:3492492). It also elevates platelet cAMP (PubMed:1318039). CGRP1 can also bind and activate CALCR-RAMP1 (AMYR1) receptor complex (PubMed:38603770).
- Sequence similarities
Belongs to the calcitonin family.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CGRP or Calcitonin Gene-Related Peptide is a 37-amino acid neuropeptide with a molecular mass of approximately 3.8 kDa. This peptide exists mainly in two forms alpha-CGRP and beta-CGRP differing slightly in amino acid sequence. CGRP expresses in peripheral and central nervous systems with high levels in the dorsal root ganglia and thyroid gland. It also has a significant presence in the enteric nervous system and some segments of the cardiovascular system. Scientists note that CGRP acts as a potent vasodilator and plays a significant role in transducing pain signals.
- Biological function summary
CGRP contributes to numerous physiological processes including blood flow regulation body temperature control pain transmission and neurogenic inflammation. It acts independently and does not substantially form part of larger protein complexes in its primary activities. Through binding to the CGRP receptor a complex made up of the calcitonin receptor-like receptor (CLR) and receptor activity-modifying protein 1 (RAMP1) CGRP initiates downstream signaling that results in its various biological effects.
- Pathways
Studies place CGRP within the neuropeptide signaling pathway and the vasodilation pathway. It significantly influences the cyclic AMP (cAMP) signaling pathway where it works collaboratively with other neuropeptides like substance P. The interaction with CLR and RAMP1 triggers the production of cAMP which enhances vasodilation and pain transmission processes in the body.
- Associated diseases and disorders
CGRP holds a notable connection to migraines and cardiovascular diseases. Elevated CGRP levels are observed in migraine sufferers and targeted treatments utilizing anti-CGRP antibodies have demonstrated efficacy in reducing migraine frequency. Additionally research links CGRP to blood pressure regulation abnormalities implicating its involvement in hypertension. Both conditions involve complex interactions with other proteins such as substance P and endothelial nitric oxide synthase (eNOS) which modulate vascular tone and inflammation.
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2 product images
SDS-PAGE - Recombinant Human CGRP Protein (GST tag) (ab318944)
SDS-PAGE analysis of ab318944 under reducing conditions for 2ug protein.
Mass Spectrometry - Recombinant Human CGRP Protein (GST tag) (ab318944)
Mass determination by ESI-TOF.
Predicted MW is 29130 Da (+/- 10 Da by ESI-TOF). Observed MW is 29125.39 Da.
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