Recombinant Human CH25H protein
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Recombinant Human CH25H protein is a Human Full Length protein, in the 1 to 272 aa range, expressed in Wheat germ, suitable for ELISA, WB.
View Alternative Names
Cholesterol 25-hydroxylase, Cholesterol 25-monooxygenase, h25OH, CH25H
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant Human CH25H protein (AB160295)
ab160295 on a 12.5% SDS-PAGE stained with Coomassie Blue.
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Cholesterol 25-hydroxylase plays a significant role in lipid metabolism and immune regulation. It produces 25-hydroxycholesterol a potent oxysterol which exerts effects on lipid metabolic processes and immune cell signaling. While not part of a large multiprotein complex CH25H's activity impacts cellular cholesterol levels and potentially influences the production of certain signaling molecules.
Pathways
Cholesterol 25-hydroxylase contributes to the regulation of lipid metabolic pathways and innate immune responses. Within the lipid metabolism pathway CH25H interacts with proteins like LXR (liver X receptor) affecting cholesterol homeostasis. The enzyme also influences immune regulation by altering the production of signaling molecules that modulate the interferon response affecting pathways related to inflammation and innate immunity.
Specifications
Form
Liquid
General info
Function
Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes (PubMed : 9852097). Plays a key role in cell positioning and movement in lymphoid tissues : 25-hydroxycholesterol is an intermediate in biosynthesis of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC), an oxysterol that acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for a number of lymphoid cells (By similarity). May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing (PubMed : 9852097). As an interferon-stimulated gene, has broad antiviral activities against a wide range of enveloped viruses, such as vesicular stomatitis virus (VSV) and SARS coronavirus-2 (SARS-CoV-2). Its product, 25-hydroxycholesterol, activates the ER-localized enzyme ACAT to induce internalization of accessible cholesterol on the plasma membrane and restricts SARS-CoV-2 S protein-mediated fusion which inhibits virus replication (PubMed : 32944968, PubMed : 33239446). In testis, production of 25-hydroxycholesterol by macrophages plays a role in Leydig cell differentiation (By similarity). Required to restrain inflammation in macrophages : production of 25-hydroxycholesterol protects macrophages from cholesterol overload, thereby preventing mitochondrial DNA release and subsequent activation of the AIM2 inflammasome (By similarity).
Sequence similarities
Belongs to the sterol desaturase family.
Post-translational modifications
N-glycosylated.
Target data
Complementary Products
Product promise
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