Recombinant Human CHCHD8 protein is a Human Full Length protein, in the 1 to 87 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M S T S V P Q G H T W T Q R V K K D D E E E D P L D Q L I S R S G C A A S H F A V Q E C M A Q H Q D W R Q C Q P Q V Q A F K D C M S E Q Q A R R Q E E L Q R R Q E Q A G A H H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Alternative names
CHCHD8, E2IG2, COA4, Coiled-coil-helix-coiled-coil-helix domain-containing protein 8, E2-induced gene 2 protein
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Recombinant Human CHCHD8 protein is a Human Full Length protein, in the 1 to 87 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q9NYJ1-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M S T S V P Q G H T W T Q R V K K D D E E E D P L D Q L I S R S G C A A S H F A V Q E C M A Q H Q D W R Q C Q P Q V Q A F K D C M S E Q Q A R R Q E E L Q R R Q E Q A G A H H
- Accession
- Q9NYJ1
- Protein length
- Full Length
- Predicted molecular weight
- 12.5 kDa
- Amino acids
- 1 to 87
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab181921 purified using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.
General info
- Function
Putative COX assembly factor.
- Sequence similarities
Belongs to the COA4 family.
- Subcellular localisation
- Mitochondrion
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CHCHD8 is a mitochondrial protein also known as Coiled-Coil-Helix-Coiled-Coil-Helix Domain Containing 8. It features a molecular mass of approximately 23 kDa. Researchers find CHCHD8 expressed predominantly in the mitochondria of skeletal muscle heart and brain tissues. This protein contributes to mitochondrial function and organization assisting in the maintenance of cellular energy and balance.
- Biological function summary
CHCHD8 acts as a component of the mitochondrial intermembrane space bridging complex (MICOS). This complex plays a pivotal role in maintaining mitochondrial architecture and integrity. CHCHD8 contributes to the folding and assembly of other mitochondrial proteins. Its interactions within the MICOS complex reveal its influence on cristae morphology which is essential for the proper function of the electron transport chain.
- Pathways
CHCHD8 takes part in mitochondrial biogenesis and cellular respiration pathways. These pathways are important for cell energy production and metabolic balance. CHCHD8 participates alongside proteins such as CHCHD3 and SAMM50 in regulating the structure and function of mitochondria integrating signals related to energy requirements and reactive oxygen species handling.
- Associated diseases and disorders
CHCHD8 has a connection with neuromuscular diseases and mitochondrial myopathies. Mutations or disruptions in CHCHD8 can lead to impaired mitochondrial function contributing to disease pathology. CHCHD8 is linked with CHCHD10 another protein implicated in similar disorders highlighting their combined importance in mitochondrial and neuromuscular health.
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1 product image
SDS-PAGE - Recombinant Human CHCHD8 protein (ab181921)
15% SDS-PAGE analysis of ab181921 (3μg).
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