Recombinant Human CHD4 protein (His tag) is a Human Fragment protein, in the 1681 to 1912 aa range, expressed in Escherichia coli, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
K D L N D E K Q K K N I K Q R F M F N I A D G G F T E L H S L W Q N E E R A A T V T K K T Y E I W H R R H D Y W L L A G I I N H G Y A R W Q D I Q N D P R Y A I L N E P F K G E M N R G N F L E I K N K F L A R R F K L L E Q A L V I E E Q L R R A A Y L N M S E D P S H P S M A L N T R F A E V E C L A E S H Q H L S K E S M A G N K P A N A V L H K V L K Q L E E L L S D M K A D V T R L P A T I A R I P P V A V R L Q M S E R N I L S R L A N R A P E P T P Q Q V A Q Q Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
ATP-dependent helicase that binds and distorts nucleosomal DNA (PubMed:28977666, PubMed:32543371). Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin (PubMed:16428440, PubMed:17626165, PubMed:28977666, PubMed:9804427). Localizes to acetylated damaged chromatin in a ZMYND8-dependent manner, to promote transcriptional repression and double-strand break repair by homologous recombination (PubMed:25593309). Involved in neurogenesis (By similarity).
Alternative names
Chromodomain-helicase-DNA-binding protein 4, CHD-4, ATP-dependent helicase CHD4, Mi-2 autoantigen 218 kDa protein, Mi2-beta, CHD4
Recommended products
Recombinant Human CHD4 protein (His tag) is a Human Fragment protein, in the 1681 to 1912 aa range, expressed in Escherichia coli, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q14839-1
- Animal free
- No
- Species
- Human
- Reconstitution
- Reconstitute in PBS.
- Concentration
- Storage buffer
pH: 8
Constituents: 82.8% Trehalose, 16.38% PBS, 0.17% Sodium-N-Lauroylsarcosinate
Sequence info
Amino acid sequence
- K D L N D E K Q K K N I K Q R F M F N I A D G G F T E L H S L W Q N E E R A A T V T K K T Y E I W H R R H D Y W L L A G I I N H G Y A R W Q D I Q N D P R Y A I L N E P F K G E M N R G N F L E I K N K F L A R R F K L L E Q A L V I E E Q L R R A A Y L N M S E D P S H P S M A L N T R F A E V E C L A E S H Q H L S K E S M A G N K P A N A V L H K V L K Q L E E L L S D M K A D V T R L P A T I A R I P P V A V R L Q M S E R N I L S R L A N R A P E P T P Q Q V A Q Q Q
- Accession
- Q14839
- Protein length
- Fragment
- Predicted molecular weight
- 30.7 kDa
- Amino acids
- 1681 to 1912
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
ATP-dependent helicase that binds and distorts nucleosomal DNA (PubMed:28977666, PubMed:32543371). Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin (PubMed:16428440, PubMed:17626165, PubMed:28977666, PubMed:9804427). Localizes to acetylated damaged chromatin in a ZMYND8-dependent manner, to promote transcriptional repression and double-strand break repair by homologous recombination (PubMed:25593309). Involved in neurogenesis (By similarity).
- Sequence similarities
Belongs to the SNF2/RAD54 helicase family.
- Subcellular localisation
- Nucleus, Cytoskeleton, Microtubule organizing center, Centrosome
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CHD4 also known as CDH4 is a protein that functions as part of the nucleosome remodeling and deacetylase (NuRD) complex. CHD4 has a molecular mass of approximately 218 kDa. The protein contains a chromo domain a helicase domain and two PHD-type zinc fingers. It is expressed ubiquitously in human tissues with higher expression levels seen in the spleen and fetal brain. This protein plays an essential role in DNA transcription regulation by modifying chromatin structure.
- Biological function summary
CHD4 acts within the NuRD complex to mediate transcriptional repression. This complex combines chromatin remodeling with histone deacetylation to alter chromatin dynamics affecting gene expression. CHD4's involvement in the complex facilitates the static organization of chromatin therefore contributing to transcriptional silencing of specific gene sets. The protein's activity is often in response to environmental and developmental cues playing an integral role in cellular differentiation and proliferation processes.
- Pathways
CHD4 connects closely with DNA damage repair and cell cycle regulation pathways. It participates actively in the DNA damage response pathway by facilitating the repair of double-strand breaks working alongside proteins such as ATM and ATR. CHD4 also influences the cell cycle by interacting with cyclins and cyclin-dependent kinases which maintain proper cell cycle progression and check points.
- Associated diseases and disorders
CHD4 has been linked to various cancers and neurodevelopmental disorders. Alterations or mutations in CHD4 have associations with certain cancers like colorectal cancer where it affects cellular proliferation and migration. In neurodevelopmental disorders disrupted CHD4 function may lead to chromatin misregulation contributing to intellectual disabilities. CHD4’s interactions with proteins such as p53 in cancer and MeCP2 in neurological conditions highlight its connections in disease mechanisms.
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SDS-PAGE - Recombinant Human CHD4 protein (His tag) (ab275792)
SDS-PAGE analysis of ab275792.
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