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AB151883

Recombinant Human CHIT1 protein

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Recombinant Human CHIT1 protein is a Human Full Length protein, in the 22 to 466 aa range, expressed in HEK 293 cells, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, HPLC.

View Alternative Names

Chitotriosidase-1, Chitinase-1, CHIT1

Key facts

Purity

>95% SDS-PAGE

Purity is greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. ab151883 is supplied as a 0.2 µm filtered solution.

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

HPLC, SDS-PAGE

applications

Biologically active

No

Accession

Q13231

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 0.88% Sodium chloride, 0.32% Tris HCl

storage-buffer

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Complementary Products

Reagents, Controls & Accessories

AB325995

Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands)

SDS-PAGE - Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands) (AB325995)

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Primary Antibodies

AB259620

Anti-CHIT1 antibody [EPR22314-23] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-CHIT1 antibody [EPR22314-23] - BSA and Azide free (Detector) (AB259620)

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Assay Kits

AB246542

Mouse CHIT1 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Mouse CHIT1 ELISA Kit (AB246542)

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Proteins & Peptides

AB152034

Recombinant Human YKL-39 protein

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  • 0
Assay Kits

AB256687

Mouse CHIT1 Antibody Pair - BSA and Azide free

RabMAb|Recombinant

Sandwich ELISA - Mouse CHIT1 Antibody Pair - BSA and Azide free (AB256687)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"linker":null,"sequence":"AKLVCYFTNWAQYRQGEARFLPKDLDPSLCTHLIYAFAGMTNHQLSTTEWNDETLYQEFNGLKKMNPKLKTLLAIGGWNFSTQKFTDMVATANNRQTFVNSAIRFLRKYSFDGLDLDWEYPGSQGSPAVDKERFTTLVQDLANAFQQEAQTSGKERLLLSAAVPAGQTYVDAGYEVDKIAQNLDFVNLMAYDFHGSWEKVTGHNSPLYKRQEESGAAASLNVDAAVQQWLQKGTPASKLILGMPTYGRSFTLASSSDTRVGAPATGSGTPGPFTKEGGMLAYYEVCSWKGATKQRIQDQKVPYIFRDNQWVGFDDVESFKTKVSYLKQKGLGGAMVWALDLDDFAGFSCNQGRYPLIQTLRQELSLPYLPSGTPELEVPKPGQPSEPEHGPSPGQDTFCQGKADGLYPNPRERSSFYSCAGGRLFQQSCPTGLVFSNSCKCCTWNVDHHHHHH","proteinLength":"Full Length","predictedMolecularWeight":"50.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":466,"aminoAcidStart":22,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q13231","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Form
Liquid
Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CHIT1 or chitotriosidase is a glycosyl hydrolase enzyme with significant roles in chitin breakdown. It has an approximate mass of 50 kDa and recognizes chitin-containing substances. CHIT1 predominantly expresses in macrophages and acts as an important enzyme in innate immunity by degrading pathologically accumulated chitin. Alternative names for CHIT1 include chitinase-1 and it is distinguishable by its active role in neutralizing fungal pathogens utilizing this chitinase mechanism.
Biological function summary

CHIT1 participates in inflammatory processes and helps in tissue remodeling which is important in immune responses. CHIT1 does not typically form part of a complex but operates independently to mediate macrophage response through its chitinase activity. It handles polysaccharide elements in cellular debris impacting macrophage activation states. Macrophages utilize CHIT1 to counteract foreign bodies engulfed during phagocytosis playing essential roles in managing fungal infections.

Pathways

CHIT1 influences both the immune response and metabolic pathways through its enzyme function. One key pathway involving CHIT1 is the mannose receptor-mediated clearance pathway which clears glycoproteins from systemic circulation. CHIT1 activity mirrors that of other chitinases like AMCase although they derive from separate genetic backgrounds. CHIT1 contribution enhances macrophage functionality within phagolysosomal pathways important for broad-spectrum pathogen eradication.

CHIT1 correlates significantly with Gaucher's disease and chronic obstructive pulmonary disease (COPD). In Gaucher's disease elevated CHIT1 levels serve as a biomarker indicating macrophage activation and infiltration. In the case of COPD CHIT1 modulates inflammatory responses that contribute to disease progression and respiratory complications. CHIT1 interacts with proteins associated with immune regulation such as TNF-alpha in these diseases impacting inflammatory pathways and disease severities.
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General info

Function

Degrades chitin, chitotriose and chitobiose. May participate in the defense against nematodes and other pathogens. Isoform 3 has no enzymatic activity.

Sequence similarities

Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily.

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Product protocols

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Target data

Degrades chitin, chitotriose and chitobiose. May participate in the defense against nematodes and other pathogens. Isoform 3 has no enzymatic activity.
See full target information Chitotriosidase-1
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Product promise

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