Recombinant Human CHMP2B protein is a Human Full Length protein, in the 1 to 213 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Images
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M A S L F K K K T V D D V I K E Q N R E L R G T Q R A I I R D R A A L E K Q E K Q L E L E I K K M A K I G N K E A C K V L A K Q L V H L R K Q K T R T F A V S S K V T S M S T Q T K V M N S Q M K M A G A M S T T A K T M Q A V N K K M D P Q K T L Q T M Q N F Q K E N M K M E M T E E M I N D T L D D I F D G S D D E E E S Q D I V N Q V L D E I G I E I S G K M A K A P S A A R S L P S A S T S K A T I S D E E I E R Q L K A L G V D
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4.
Alternative names
CGI-84, CHMP2B, Charged multivesicular body protein 2b, CHMP2.5, Chromatin-modifying protein 2b, Vacuolar protein sorting-associated protein 2-2, CHMP2b, Vps2-2, hVps2-2
Recommended products
Recombinant Human CHMP2B protein is a Human Full Length protein, in the 1 to 213 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q9UQN3-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M A S L F K K K T V D D V I K E Q N R E L R G T Q R A I I R D R A A L E K Q E K Q L E L E I K K M A K I G N K E A C K V L A K Q L V H L R K Q K T R T F A V S S K V T S M S T Q T K V M N S Q M K M A G A M S T T A K T M Q A V N K K M D P Q K T L Q T M Q N F Q K E N M K M E M T E E M I N D T L D D I F D G S D D E E E S Q D I V N Q V L D E I G I E I S G K M A K A P S A A R S L P S A S T S K A T I S D E E I E R Q L K A L G V D
- Accession
- Q9UQN3
- Protein length
- Full Length
- Predicted molecular weight
- 26.1 kDa
- Amino acids
- 1 to 213
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab109959 was purified using conventional chromatography techniques.
General info
- Function
Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4.
- Sequence similarities
Belongs to the SNF7 family.
- Subcellular localisation
- Late endosome membrane
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human CHMP2B protein (ab109959)
15% SDS-PAGE analysis of 3 μg ab109959.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com