Recombinant Human CHMP5 protein is a Human Full Length protein, in the 1 to 219 aa range, expressed in Escherichia coli, with >80% purity and suitable for SDS-PAGE, MS.
Images
Key facts
- Purity
- >80% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S H M N R L F G K A K P K A P P P S L T D C I G T V D S R A E S I D K K I S R L D A E L V K Y K D Q I K K M R E G P A K N M V K Q K A L R V L K Q K R M Y E Q Q R D N L A Q Q S F N M E Q A N Y T I Q S L K D T K T T V D A M K L G V K E M K K A Y K Q V K I D Q I E D L Q D Q L E D M M E D A N E I Q E A L S R S Y G T P E L D E D D L E A E L D A L G D E L L A D E D S S Y L D E A A S A P A I P E G V P T D T K N K D G V L V D E F G L P Q I P A S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses) (PubMed:14519844). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in HIV-1 p6- and p9-dependent virus release (PubMed:14519844).
Alternative names
C9orf83, SNF7DC2, CGI-34, HSPC177, PNAS-114, PNAS-2, CHMP5, Charged multivesicular body protein 5, Chromatin-modifying protein 5, SNF7 domain-containing protein 2, Vacuolar protein sorting-associated protein 60, Vps60, hVps60
Recommended products
Recombinant Human CHMP5 protein is a Human Full Length protein, in the 1 to 219 aa range, expressed in Escherichia coli, with >80% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >80% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q9NZZ3-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 30% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S H M N R L F G K A K P K A P P P S L T D C I G T V D S R A E S I D K K I S R L D A E L V K Y K D Q I K K M R E G P A K N M V K Q K A L R V L K Q K R M Y E Q Q R D N L A Q Q S F N M E Q A N Y T I Q S L K D T K T T V D A M K L G V K E M K K A Y K Q V K I D Q I E D L Q D Q L E D M M E D A N E I Q E A L S R S Y G T P E L D E D D L E A E L D A L G D E L L A D E D S S Y L D E A A S A P A I P E G V P T D T K N K D G V L V D E F G L P Q I P A S
- Accession
- Q9NZZ3
- Protein length
- Full Length
- Predicted molecular weight
- 27 kDa
- Amino acids
- 1 to 219
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab134604 was purified using conventional chromatography techniques.
General info
- Function
Probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses) (PubMed:14519844). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in HIV-1 p6- and p9-dependent virus release (PubMed:14519844).
- Sequence similarities
Belongs to the SNF7 family.
- Post-translational modifications
(Microbial infection) Stearoylated By S.flexneri N-epsilon-fatty acyltransferase IcsB, promoting S.flexneri evasion of autophagy.
- Subcellular localisation
- Endosome membrane
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
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1 product image
SDS-PAGE - Recombinant Human CHMP5 protein (ab134604)
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Downloads
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