Recombinant Human CHMP6/VPS20 protein is a Human Full Length protein, in the 1 to 201 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S H M G N L F G R K K Q S R V T E Q D K A I L Q L K Q Q R D K L R Q Y Q K R I A Q Q L E R E R A L A R Q L L R D G R K E R A K L L L K K K R Y Q E Q L L D R T E N Q I S S L E A M V Q S I E F T Q I E M K V M E G L Q F G N E C L N K M H Q V M S I E E V E R I L D E T Q E A V E Y Q R Q I D E L L A G S F T Q E D E D A I L E E L S A I T Q E Q I E L P E V P S E P L P E K I P E N V P V K A R P R Q A E L V A A S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. In the ESCRT-III complex, it probably serves as an acceptor for the ESCRT-II complex on endosomal membranes.
Alternative names
VPS20, CHMP6, Charged multivesicular body protein 6, Chromatin-modifying protein 6, Vacuolar protein sorting-associated protein 20, Vps20, hVps20
Recommended products
Recombinant Human CHMP6/VPS20 protein is a Human Full Length protein, in the 1 to 201 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q96FZ7-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S H M G N L F G R K K Q S R V T E Q D K A I L Q L K Q Q R D K L R Q Y Q K R I A Q Q L E R E R A L A R Q L L R D G R K E R A K L L L K K K R Y Q E Q L L D R T E N Q I S S L E A M V Q S I E F T Q I E M K V M E G L Q F G N E C L N K M H Q V M S I E E V E R I L D E T Q E A V E Y Q R Q I D E L L A G S F T Q E D E D A I L E E L S A I T Q E Q I E L P E V P S E P L P E K I P E N V P V K A R P R Q A E L V A A S
- Accession
- Q96FZ7
- Protein length
- Full Length
- Predicted molecular weight
- 26.1 kDa
- Amino acids
- 1 to 201
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab124606 is purified by using conventional chromatography techniques.
General info
- Function
Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. In the ESCRT-III complex, it probably serves as an acceptor for the ESCRT-II complex on endosomal membranes.
- Sequence similarities
Belongs to the SNF7 family.
- Post-translational modifications
ISGylated in a CHMP5-dependent manner. Isgylation weakens its interaction with VPS4A.
- Subcellular localisation
- Endosome membrane, Late endosome membrane
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as CHMP6
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1 product image
SDS-PAGE - Recombinant Human CHMP6/VPS20 protein (ab124606)
3ug by SDS-PAGE under reducing conditions and visualized by coomassie blue stain.
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Product protocols
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