JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB156724

Recombinant Human CHRAC-17 protein (His tag)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human CHRAC-17 protein (His tag) is a Human Full Length protein, in the 1 to 147 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

CHRAC17, POLE3, DNA polymerase epsilon subunit 3, Arsenic-transactivated protein, Chromatin accessibility complex 17 kDa protein, DNA polymerase II subunit 3, DNA polymerase epsilon subunit p17, AsTP, CHRAC-17, HuCHRAC17

2 Images
SDS-PAGE - Recombinant Human CHRAC-17 protein (His tag) (AB156724)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human CHRAC-17 protein (His tag) (AB156724)
SDS-PAGE - Recombinant Human CHRAC-17 protein (His tag) (AB156724)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human CHRAC-17 protein (His tag) (AB156724)

15% SDS-PAGE analysis of ab156724 (3 μg).

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Q9NRF9

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Protein previously known as DNA Polymerase epsilon.
style
left-column

Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMAERPEDLNLPNAVITRIIKEALPDGVNISKEARSAISRAASVFVLYATSCANNFAMKGKRKTLNASDVLSAMEEMEFQRFVTPLKEALEAYRREQKGKKEASEQKKKDKDKKTDSEEQDKSRDEDNDEDEERLEEEEQNEEEEVDN","proteinLength":"Full Length","predictedMolecularWeight":"19 kDa","actualMolecularWeight":null,"aminoAcidEnd":147,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9NRF9","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column
style
left-column

Specifications

Form

Liquid

Additional notes

ab156724 is purified using conventional chromatography techniques (anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA).

style
left-column

General info

Function

Accessory component of the DNA polymerase epsilon complex (PubMed : 10801849). Participates in DNA repair and in chromosomal DNA replication (By similarity). Forms a complex with CHRAC1 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome-remodeling activity of ISWI/SNF2H and ACF1 (PubMed : 10801849). Does not enhance nucleosome sliding activity of the ACF-5 ISWI chromatin remodeling complex (PubMed : 14759371).

Subcellular localisation

Nucleus

style
left-column

Product protocols

style
left-column

Target data

Accessory component of the DNA polymerase epsilon complex (PubMed : 10801849). Participates in DNA repair and in chromosomal DNA replication (By similarity). Forms a complex with CHRAC1 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome-remodeling activity of ISWI/SNF2H and ACF1 (PubMed : 10801849). Does not enhance nucleosome sliding activity of the ACF-5 ISWI chromatin remodeling complex (PubMed : 14759371).
See full target information POLE3
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB229060

Anti-CHRAC-17 antibody

Immunocytochemistry/ Immunofluorescence - Anti-CHRAC-17 antibody (AB229060)

  • 0
  • 0
Cell Lines & Lysates

AB266096

Human POLE3 (CHRAC-17) knockout HEK-293T cell line

Sanger Sequencing - Human POLE3 (CHRAC-17) knockout HEK-293T cell line (AB266096)

  • 0
  • 0
Cell Lines & Lysates

AB258126

Human POLE3 (CHRAC-17) knockout HEK-293T cell lysate

Sanger Sequencing - Human POLE3 (CHRAC-17) knockout HEK-293T cell lysate (AB258126)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com