AB85486

Recombinant Human Chromogranin A protein (His tag N-Terminus)

Name: Recombinant Human Chromogranin A protein (His tag N-Terminus) (ab85486) | Abcam
Brand: Abcam Limited
SKU: AB85486

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(3 Publications)

Recombinant Human Chromogranin A protein is a Human Full Length CHGA protein, in the 19 to 457 aa range with >80% purity and suitable for western blot and SDS-PAGE. The observed molecular weight of ab85486 recombinant protein will appear higher than 51.2 kDa.

- Save time and ensure accurate results - use our recombinant Chromogranin A (CHGA) protein as a control
- Available in different sizes to fit your experimental needs

View Alternative Names

Chromogranin-A, CgA, Pituitary secretory protein I, SP-I, CHGA

3 Images

Sandwich ELISA - Recombinant Human Chromogranin A protein (AB85486)

sELISA

Unknown

Sandwich ELISA - Recombinant Human Chromogranin A protein (AB85486)

Standard curve for Chromogranin A (Analyte : ab85486); dilution range 1pg/ml to 1μg/ml using Capture Antibody ab36997 at 1μg/ml and Detector Antibody ab17064 at 0.5μg/ml. Concentration of ab17064 may vary from lot to lot; please use this curve as guideline.

Unknown

Western blot - Recombinant Human Chromogranin A protein (His tag N-Terminus) (AB85486)

Chromogranin A is easily fragmented and both bands observed in the Western Blot above are believed to correspond to Chromogranin A.

All lanes:

Western blot - Anti-Chromogranin A antibody (<a href='/en-us/products/primary-antibodies/chromogranin-a-antibody-ab15160'>ab15160</a>) at 1/100 dilution

All lanes:

Western blot - Recombinant Human Chromogranin A protein (His tag N-Terminus) (ab85486) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 50 kDa

true

Exposure time: 30s

SDS-PAGE - Recombinant Human Chromogranin A protein (AB85486)

SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Chromogranin A protein (AB85486)

12% SDS-PAGE analysis of 3μg of ab85486

The ab85486 predicted molecular weight is 51.2 kDa. The observed molecular weight on SDS-PAGE will appear higher.

Key facts

Purity

>80% SDS-PAGE

Expression system

Escherichia coli

Applications

WB, SDS-PAGE

applications

Biologically active

Accession

P10645

Animal free

Carrier free

Species

Human

Storage buffer

pH: 7.5 Constituents: 10% Glycerol (glycerin, glycerine), 0.39% MES, 0.0584% EDTA, 0.00174% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab85486 can be used as a WB positive control in conjunction with <a href='/en-us/products/primary-antibodies/chromogranin-a-antibody-ab15160'>ab15160</a>.</p>" } } }

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Product details

Ensure the validity of your result using our recombinant human Chromogranin A protein ab85486 as a control:
Chromogranin A protein ab85486 can be used as a positive control in SDS-PAGE and western blot assays.
Analyze the Chromogranin A protein levels in your ELISA data using the ab85486 to generate and plot the standard curve.
In Western blot ab85486 antibody can be used to quantify Chromogranin A protein expression in tissues under different experimental conditions.

The ab85486 concentration was determined by Bradford assay.

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Sequence info

[{"sequence":"MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSHMLPVNSPMNKGDTEVMKCIVEVISDTLSKPSPMPVSQECFETLRGDERILSILRHQNLLKELQDLALQGAKERAHQQKKHSGFEDELSEVLENQSSQAELKEAVEEPSSKDVMEKREDSKEAEKSGEATDGARPQALPEPMQESKAEGNNQAPGEEEEEEEEATNTHPPASLPSQKYPGPQAEGDSEGLSQGLVDREKGLSAEPGWQAKREEEEEEEEEAEAGEEAVPEEEGPTVVLNPHPSLGYKEIRKGESRSEALAVDGAGKPGAEEAQDPEGKGEQEHSQQKEEEEEMAVVPQGLFRGGKSGELEQEEERLSKEWEDSKRWSKMDQLAKELTAEKRLEGQEEEEDNRDSSMKLSFRARAYGFRGPGPQLRRGWRPSSREDSLEAGLPLQVRGYPEEKKEEEGSANRRPEDQELESLSAIEAELEKVAHQLQALRRG","proteinLength":"Full Length","predictedMolecularWeight":"51.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":457,"aminoAcidStart":19,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P10645","tags":[{"tag":"His","terminus":"N-Terminus"}]}]

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Properties and storage information

Shipped at conditions

Dry Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

False

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Chromogranin A (CgA) sometimes called chromagranin A is a glycoprotein with a molecular weight of approximately 49–52 kDa. It is highly expressed in the secretory granules of neuroendocrine cells present in tissues such as the adrenal medulla pancreas and endocrine cells of the gastrointestinal tract. CgA identified as part of a family including chromogranin B and chromogranin C functions mechanically as a precursor to various bioactive peptides. This translates into its involvement in the storage and release of hormones and peptides within vesicles.

Biological function summary

Chromogranin A assists in the regulation of secretory pathways across multiple neuroendocrine systems. It plays a role in hormone storage conversion to active peptides and regulates the exocytosis of hormones. CgA acts within a complex contributing to secretory granule biogenesis and influencing intravesicular acidity. As a regulatory component the exact physiological roles are diverse impacting cardiovascular metabolic and immunological processes.

Pathways

Chromogranin A is involved in pathways such as the catecholamine synthesis and release pathway and the serotonergic pathway. Within these pathways it interacts with proteins like secretogranin II and parathyroid hormone (PTH). These interactions modulate neurotransmitter storage and secretion across various systems. CgA's bioactive peptides also contribute to modulating physiological processes like vasoconstriction and insulin regulation linking it closely with these signaling cascades.

Chromogranin A is a known marker for neuroendocrine tumors where its levels in blood may increase due to tumor activity. It links to diseases like pheochromocytoma and carcinoid tumors where improper regulation of CgA-associated pathways occurs. Additionally its connection to parathyroid disorders through the PTH pathway illustrates its broader implication in endocrine pathophysiology. CgA serves as an invaluable diagnostic and prognostic tool in these disease states and supports the development of targeted therapies.

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Specifications

Form

Liquid

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General info

Function

Pancreastatin. Strongly inhibits glucose induced insulin release from the pancreas.. Catestatin. Inhibits catecholamine release from chromaffin cells and noradrenergic neurons by acting as a non-competitive nicotinic cholinergic antagonist (PubMed : 15326220). Displays antibacterial activity against Gram-positive bacteria S.aureus and M.luteus, and Gram-negative bacteria E.coli and P.aeruginosa (PubMed : 15723172, PubMed : 24723458). Can induce mast cell migration, degranulation and production of cytokines and chemokines (PubMed : 21214543). Acts as a potent scavenger of free radicals in vitro (PubMed : 24723458). May play a role in the regulation of cardiac function and blood pressure (PubMed : 18541522).. Serpinin. Regulates granule biogenesis in endocrine cells by up-regulating the transcription of protease nexin 1 (SERPINE2) via a cAMP-PKA-SP1 pathway. This leads to inhibition of granule protein degradation in the Golgi complex which in turn promotes granule formation.

Sequence similarities

Belongs to the chromogranin/secretogranin protein family.

Post-translational modifications

Sulfated on tyrosine residues and/or contains sulfated glycans.. O-glycosylated with core 1 or possibly core 8 glycans (PubMed:19838169, PubMed:23234360, PubMed:9852066). Contains chondroitin sulfate (CS); CS attachment is pH-dependent, being observed at mildly acidic conditions of pH 5 but not at neutral pH, and promotes self-assembly in vitro (PubMed:25326458).. Proteolytic processing gives rise to an additional longer form of catestatin (residues 358-390) which displays a less potent catecholamine release-inhibitory activity (PubMed:10781584). Plasmin-mediated proteolytic processing can give rise to additional shorter and longer forms of catestatin peptides (PubMed:17991725).

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information CHGA

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of the American Society for Mass Spectrometry 34:649-667 PubMed36912488

2023

Cerebrospinal Fluid and Brain Proteoforms of the Granin Neuropeptide Family in Alzheimer's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

James P Quinn,Elizabeth C Ethier,Angelo Novielli,Aygul Malone,Christopher E Ramirez,Lauren Salloum,Bianca A Trombetta,Pia Kivisäkk,Michael Bremang,Stefan Selzer,Marjorie Fournier,Sudeshna Das,Yaoyi Xing,Steven E Arnold,Becky C Carlyle

Alzheimer's & dementia : the journal of the Alzheimer's Association 17:1976-1987 PubMed33984181

2021

Longitudinal CSF proteomics identifies NPTX2 as a prognostic biomarker of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Ondrej Libiger,Leslie M Shaw,Mark H Watson,Angus C Nairn,Kelly L Umaña,Michael C Biarnes,Rosa M Canet-Avilés,Clifford R Jack,Yannick-André Breton,Laetitia Cortes,Daniel Chelsky,Daniel S Spellman,Susan A Baker,Nandini Raghavan,William Z Potter

Molecular & cellular proteomics : MCP 14:41-9 PubMed25326458

2014

Identification of chondroitin sulfate linkage region glycopeptides reveals prohormones as a novel class of proteoglycans.

Applications

Unspecified application

Species

Unspecified reactive species

Fredrik Noborn,Alejandro Gomez Toledo,Carina Sihlbom,Johan Lengqvist,Erik Fries,Lena Kjellén,Jonas Nilsson,Göran Larson

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Recombinant Human Chromogranin A protein (His tag N-Terminus)

Sandwich ELISA - Recombinant Human Chromogranin A protein (AB85486)

Western blot - Recombinant Human Chromogranin A protein (His tag N-Terminus) (AB85486)

SDS-PAGE - Recombinant Human Chromogranin A protein (AB85486)

Key facts

Purity

Expression system

Tags

Applications

Biologically active

Accession

Animal free

Carrier free

Species

Storage buffer

Reactivity data

Product details

Sequence info

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Specifications

Form

General info

Function

Sequence similarities

Post-translational modifications

Product protocols

Target data

Publications (3)

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

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