Recombinant Human Chromogranin A protein is a Human Full Length CHGA protein, in the 19 to 457 aa range with >80% purity and suitable for western blot and SDS-PAGE. The observed molecular weight of ab85486 recombinant protein will appear higher than 51.2 kDa.
- Save time and ensure accurate results - use our recombinant Chromogranin A (CHGA) protein as a control
- Available in different sizes to fit your experimental needs
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Publications
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- Journal of the American Society for Mass Spectrometry 34:649-6672023Cerebrospinal Fluid and Brain Proteoforms of the Granin Neuropeptide Family in Alzheimer's Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJames P Quinn,Elizabeth C Ethier,Angelo Novielli,Aygul Malone,Christopher E Ramirez,Lauren Salloum,Bianca A Trombetta,Pia Kivisäkk,Michael Bremang,Stefan Selzer,Marjorie Fournier,Sudeshna Das,Yaoyi Xing,Steven E Arnold,Becky C CarlylePubMed 36912488
- Alzheimer's & dementia : the journal of the Alzheimer's Association 17:1976-19872021Longitudinal CSF proteomics identifies NPTX2 as a prognostic biomarker of Alzheimer's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOndrej Libiger,Leslie M Shaw,Mark H Watson,Angus C Nairn,Kelly L Umaña,Michael C Biarnes,Rosa M Canet-Avilés,Clifford R Jack,Yannick-André Breton,Laetitia Cortes,Daniel Chelsky,Daniel S Spellman,Susan A Baker,Nandini Raghavan,William Z PotterPubMed 33984181
- Molecular & cellular proteomics : MCP 14:41-92014Identification of chondroitin sulfate linkage region glycopeptides reveals prohormones as a novel class of proteoglycans.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFredrik Noborn,Alejandro Gomez Toledo,Carina Sihlbom,Johan Lengqvist,Erik Fries,Lena Kjellén,Jonas Nilsson,Göran LarsonPubMed 25326458
Key facts
- Purity
- >80% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, WB
- Biologically active
- No
Amino acid sequence
M R G S H H H H H H G M A S M T G G Q Q M G R D L Y D D D D K D R W G S H M L P V N S P M N K G D T E V M K C I V E V I S D T L S K P S P M P V S Q E C F E T L R G D E R I L S I L R H Q N L L K E L Q D L A L Q G A K E R A H Q Q K K H S G F E D E L S E V L E N Q S S Q A E L K E A V E E P S S K D V M E K R E D S K E A E K S G E A T D G A R P Q A L P E P M Q E S K A E G N N Q A P G E E E E E E E E A T N T H P P A S L P S Q K Y P G P Q A E G D S E G L S Q G L V D R E K G L S A E P G W Q A K R E E E E E E E E E A E A G E E A V P E E E G P T V V L N P H P S L G Y K E I R K G E S R S E A L A V D G A G K P G A E E A Q D P E G K G E Q E H S Q Q K E E E E E M A V V P Q G L F R G G K S G E L E Q E E E R L S K E W E D S K R W S K M D Q L A K E L T A E K R L E G Q E E E E D N R D S S M K L S F R A R A Y G F R G P G P Q L R R G W R P S S R E D S L E A G L P L Q V R G Y P E E K K E E E G S A N R R P E D Q E L E S L S A I E A E L E K V A H Q L Q A L R R G
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes ab85486 can be used as a WB positive control in conjunction with Anti-Chromogranin A antibody ab15160. |
Associated Products
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Target data
Function
Pancreastatin. Strongly inhibits glucose induced insulin release from the pancreas. Catestatin. Inhibits catecholamine release from chromaffin cells and noradrenergic neurons by acting as a non-competitive nicotinic cholinergic antagonist (PubMed:15326220). Displays antibacterial activity against Gram-positive bacteria S.aureus and M.luteus, and Gram-negative bacteria E.coli and P.aeruginosa (PubMed:15723172, PubMed:24723458). Can induce mast cell migration, degranulation and production of cytokines and chemokines (PubMed:21214543). Acts as a potent scavenger of free radicals in vitro (PubMed:24723458). May play a role in the regulation of cardiac function and blood pressure (PubMed:18541522). Serpinin. Regulates granule biogenesis in endocrine cells by up-regulating the transcription of protease nexin 1 (SERPINE2) via a cAMP-PKA-SP1 pathway. This leads to inhibition of granule protein degradation in the Golgi complex which in turn promotes granule formation.
Alternative names
Chromogranin-A, CgA, Pituitary secretory protein I, SP-I, CHGA
Recommended products
Recombinant Human Chromogranin A protein is a Human Full Length CHGA protein, in the 19 to 457 aa range with >80% purity and suitable for western blot and SDS-PAGE. The observed molecular weight of ab85486 recombinant protein will appear higher than 51.2 kDa.
- Save time and ensure accurate results - use our recombinant Chromogranin A (CHGA) protein as a control
- Available in different sizes to fit your experimental needs
Key facts
- Purity
- >80% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, WB
- Biologically active
- No
- Accession
- P10645-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 10% Glycerol (glycerin, glycerine), 0.39% MES, 0.0584% EDTA, 0.00174% PMSF
Sequence info
Amino acid sequence
- M R G S H H H H H H G M A S M T G G Q Q M G R D L Y D D D D K D R W G S H M L P V N S P M N K G D T E V M K C I V E V I S D T L S K P S P M P V S Q E C F E T L R G D E R I L S I L R H Q N L L K E L Q D L A L Q G A K E R A H Q Q K K H S G F E D E L S E V L E N Q S S Q A E L K E A V E E P S S K D V M E K R E D S K E A E K S G E A T D G A R P Q A L P E P M Q E S K A E G N N Q A P G E E E E E E E E A T N T H P P A S L P S Q K Y P G P Q A E G D S E G L S Q G L V D R E K G L S A E P G W Q A K R E E E E E E E E E A E A G E E A V P E E E G P T V V L N P H P S L G Y K E I R K G E S R S E A L A V D G A G K P G A E E A Q D P E G K G E Q E H S Q Q K E E E E E M A V V P Q G L F R G G K S G E L E Q E E E R L S K E W E D S K R W S K M D Q L A K E L T A E K R L E G Q E E E E D N R D S S M K L S F R A R A Y G F R G P G P Q L R R G W R P S S R E D S L E A G L P L Q V R G Y P E E K K E E E G S A N R R P E D Q E L E S L S A I E A E L E K V A H Q L Q A L R R G
- Accession
- P10645
- Protein length
- Full Length
- Predicted molecular weight
- 51.2 kDa
- Amino acids
- 19 to 457
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Pancreastatin
- Sequence similarities
Belongs to the chromogranin/secretogranin protein family.
- Post-translational modifications
Sulfated on tyrosine residues and/or contains sulfated glycans.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Ensure the validity of your result using our recombinant human Chromogranin A protein ab85486 as a control:
Chromogranin A protein ab85486 can be used as a positive control in SDS-PAGE and western blot assays.
Analyze the Chromogranin A protein levels in your ELISA data using the ab85486 to generate and plot the standard curve.
In Western blot ab85486 antibody can be used to quantify Chromogranin A protein expression in tissues under different experimental conditions.
The ab85486 concentration was determined by Bradford assay.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Chromogranin A (CgA) sometimes called chromagranin A is a glycoprotein with a molecular weight of approximately 49–52 kDa. It is highly expressed in the secretory granules of neuroendocrine cells present in tissues such as the adrenal medulla pancreas and endocrine cells of the gastrointestinal tract. CgA identified as part of a family including chromogranin B and chromogranin C functions mechanically as a precursor to various bioactive peptides. This translates into its involvement in the storage and release of hormones and peptides within vesicles.
- Biological function summary
Chromogranin A assists in the regulation of secretory pathways across multiple neuroendocrine systems. It plays a role in hormone storage conversion to active peptides and regulates the exocytosis of hormones. CgA acts within a complex contributing to secretory granule biogenesis and influencing intravesicular acidity. As a regulatory component the exact physiological roles are diverse impacting cardiovascular metabolic and immunological processes.
- Pathways
Chromogranin A is involved in pathways such as the catecholamine synthesis and release pathway and the serotonergic pathway. Within these pathways it interacts with proteins like secretogranin II and parathyroid hormone (PTH). These interactions modulate neurotransmitter storage and secretion across various systems. CgA's bioactive peptides also contribute to modulating physiological processes like vasoconstriction and insulin regulation linking it closely with these signaling cascades.
- Associated diseases and disorders
Chromogranin A is a known marker for neuroendocrine tumors where its levels in blood may increase due to tumor activity. It links to diseases like pheochromocytoma and carcinoid tumors where improper regulation of CgA-associated pathways occurs. Additionally its connection to parathyroid disorders through the PTH pathway illustrates its broader implication in endocrine pathophysiology. CgA serves as an invaluable diagnostic and prognostic tool in these disease states and supports the development of targeted therapies.
Product promise
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Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Recombinant Human Chromogranin A protein (ab85486)
Chromogranin A is easily fragmented and both bands observed in the Western Blot above are believed to correspond to Chromogranin A.
All lanes: Western blot - Anti-Chromogranin A antibody (Anti-Chromogranin A antibody ab15160) at 1/100 dilution
All lanes: Western blot - Recombinant Human Chromogranin A protein (ab85486) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Exposure time: 30s
Sandwich ELISA - Recombinant Human Chromogranin A protein (ab85486)
Standard curve for Chromogranin A (Analyte: ab85486); dilution range 1pg/ml to 1μg/ml using Capture Antibody ab36997 at 1μg/ml and Detector Antibody Anti-Chromogranin A antibody ab17064 at 0.5μg/ml. Concentration of Anti-Chromogranin A antibody ab17064 may vary from lot to lot; please use this curve as guideline.
SDS-PAGE - Recombinant Human Chromogranin A protein (ab85486)
12% SDS-PAGE analysis of 3μg of ab85486
The ab85486 predicted molecular weight is 51.2 kDa. The observed molecular weight on SDS-PAGE will appear higher.
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