JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB140542

Recombinant Human CIAO1 protein

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human CIAO1 protein is a Human Full Length protein, in the 1 to 339 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

CIA1, WDR39, CIAO1, Probable cytosolic iron-sulfur protein assembly protein CIAO1, WD repeat-containing protein 39

1 Images
SDS-PAGE - Recombinant Human CIAO1 protein (AB140542)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human CIAO1 protein (AB140542)

15% SDS-PAGE analysis of ab140542 (3ug)

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

O76071

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMKDSLVLLGRVPAHPDSRCWFLAWNPAGTLLASCGGDRRIRIWGTEGDSWICKSVLSEGHQRTVRKVAWSPCGNYLASASFDATTCIWKKNQDDFECVTTLEGHENEVKSVAWAPSGNLLATCSRDKSVWVWEVDEEDEYECVSVLNSHTQDVKHVVWHPSQELLASASYDDTVKLYREEEDDWVCCATLEGHESTVWSLAFDPSGQRLASCSDDRTVRIWRQYLPGNEQGVACSGSDPSWKCICTLSGFHSRTIYDIAWCQLTGALATACGDDAIRVFQEDPNSDPQQPTFSLTAHLHQAHSQDVNCVAWNPKEPGLLASCSDDGEVAFWKYQRPEGL","proteinLength":"Full Length","predictedMolecularWeight":"40 kDa","actualMolecularWeight":null,"aminoAcidEnd":339,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O76071","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column
style
left-column

Specifications

Form

Liquid

Additional notes

ab140542 is purified using conventional chromatography techniques.

style
left-column

General info

Function

Key component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into extramitochondrial Fe/S proteins (PubMed : 17937914, PubMed : 23891004). As a CIA complex component, interacts specifically with CIAO2A or CIAO2B and MMS19 to assist different branches of iron-sulfur protein assembly, depending of its interactors. The complex CIAO1 : CIAO2B : MMS19 binds to and facilitates the assembly of most cytosolic-nuclear Fe/S proteins. CIAO1 : CIAO2A specifically matures ACO1 and stabilizes IREB2 (PubMed : 23891004). Seems to specifically modulate the transactivation activity of WT1 (PubMed : 9556563). As part of the mitotic spindle-associated MMXD complex it may play a role in chromosome segregation (PubMed : 20797633).

Sequence similarities

Belongs to the WD repeat CIA1 family.

style
left-column

Product protocols

style
left-column

Target data

Key component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into extramitochondrial Fe/S proteins (PubMed : 17937914, PubMed : 23891004). As a CIA complex component, interacts specifically with CIAO2A or CIAO2B and MMS19 to assist different branches of iron-sulfur protein assembly, depending of its interactors. The complex CIAO1 : CIAO2B : MMS19 binds to and facilitates the assembly of most cytosolic-nuclear Fe/S proteins. CIAO1 : CIAO2A specifically matures ACO1 and stabilizes IREB2 (PubMed : 23891004). Seems to specifically modulate the transactivation activity of WT1 (PubMed : 9556563). As part of the mitotic spindle-associated MMXD complex it may play a role in chromosome segregation (PubMed : 20797633).
See full target information CIAO1
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB108257

Anti-ADX antibody [EPR4629]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-ADX antibody [EPR4629] (AB108257)

  • 5
  • 1
Primary Antibodies

AB58724

Anti-Lipoic Acid antibody

  • 0
  • 0
Primary Antibodies

AB110321

Anti-Aconitase 2 antibody [6F12BD9]

BOND RX™ Validated|KO Validated

Western blot - Anti-Aconitase 2 antibody [6F12BD9] (AB110321)

  • 5
  • 4
Primary Antibodies

AB110330

Anti-PDHA1 antibody [9H9AF5]

KO Validated

Western blot - Anti-PDHA1 antibody [9H9AF5] (AB110330)

  • 5
  • 8

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com