Recombinant Human CINP protein is a Human Full Length protein, in the 1 to 212 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M E A K T L G T V T P R K P V L S V S A R K I K D N A A D W H N L I L K W E T L N D A G F T T A N N I A N L K I S L L N K D K I E L D S S S P A S K E N E E K V C L E Y N E E L E K L C E E L Q A T L D G L T K I Q V K M E K L S S T T K G I C E L E N Y H Y G E E S K R P P L F H T W P T T H F Y E V S H K L L E M Y R K E L L L K R T V A K E L A H T G D P D L T L S Y L S M W L H Q P Y V E S D S R L H L E S M L L E T G H R A L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Component of the DNA replication complex, which interacts with two kinases, CDK2 and CDC7, thereby providing a functional and physical link between CDK2 and CDC7 during firing of the origins of replication (PubMed:16082200, PubMed:19889979). Regulates ATR-mediated checkpoint signaling in response to DNA damage (PubMed:16082200, PubMed:19889979). Part of the 55LCC heterohexameric ATPase complex which is chromatin-associated and promotes replisome proteostasis to maintain replication fork progression and genome stability. Required for replication fork progression, sister chromatid cohesion, and chromosome stability. The ATPase activity is specifically enhanced by replication fork DNA and is coupled to cysteine protease-dependent cleavage of replisome substrates in response to replication fork damage. Uses ATPase activity to process replisome substrates in S-phase, facilitating their proteolytic turnover from chromatin to ensure DNA replication and mitotic fidelity (PubMed:38554706). As part of 55LCC complex, also involved in the cytoplasmic maturation steps of pre-60S ribosomal particles by promoting the release of shuttling protein RSL24D1/RLP24 from the pre-ribosomal particles (PubMed:35354024).
Alternative names
Cyclin-dependent kinase 2-interacting protein, CDK2-interacting protein, CINP
Recommended products
Recombinant Human CINP protein is a Human Full Length protein, in the 1 to 212 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q9BW66-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.058% Sodium chloride, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M E A K T L G T V T P R K P V L S V S A R K I K D N A A D W H N L I L K W E T L N D A G F T T A N N I A N L K I S L L N K D K I E L D S S S P A S K E N E E K V C L E Y N E E L E K L C E E L Q A T L D G L T K I Q V K M E K L S S T T K G I C E L E N Y H Y G E E S K R P P L F H T W P T T H F Y E V S H K L L E M Y R K E L L L K R T V A K E L A H T G D P D L T L S Y L S M W L H Q P Y V E S D S R L H L E S M L L E T G H R A L
- Accession
- Q9BW66
- Protein length
- Full Length
- Predicted molecular weight
- 26.4 kDa
- Amino acids
- 1 to 212
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab109973 was purified using conventional chromatography.
General info
- Function
Component of the DNA replication complex, which interacts with two kinases, CDK2 and CDC7, thereby providing a functional and physical link between CDK2 and CDC7 during firing of the origins of replication (PubMed:16082200, PubMed:19889979). Regulates ATR-mediated checkpoint signaling in response to DNA damage (PubMed:16082200, PubMed:19889979). Part of the 55LCC heterohexameric ATPase complex which is chromatin-associated and promotes replisome proteostasis to maintain replication fork progression and genome stability. Required for replication fork progression, sister chromatid cohesion, and chromosome stability. The ATPase activity is specifically enhanced by replication fork DNA and is coupled to cysteine protease-dependent cleavage of replisome substrates in response to replication fork damage. Uses ATPase activity to process replisome substrates in S-phase, facilitating their proteolytic turnover from chromatin to ensure DNA replication and mitotic fidelity (PubMed:38554706). As part of 55LCC complex, also involved in the cytoplasmic maturation steps of pre-60S ribosomal particles by promoting the release of shuttling protein RSL24D1/RLP24 from the pre-ribosomal particles (PubMed:35354024).
- Sequence similarities
Belongs to the CINP family.
- Post-translational modifications
Phosphorylated by CDC7 but not by CDK2.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
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1 product image
SDS-PAGE - Recombinant Human CINP protein (ab109973)
15% SDS-PAGE analysis of 3 μg ab109973.
Downloads
Product protocols
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