Recombinant Human CISH/CIS protein is a Human Full Length protein, in the 1 to 258 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Images
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M V L C V Q G P R P L L A V E R T G Q R P L W A P S L E L P K P V M Q P L P A G A F L E E V A E G T P A Q T E S E P K V L D P E E D L L C I A K T F S Y L R E S G W Y W G S I T A S E A R Q H L Q K M P E G T F L V R D S T H P S Y L F T L S V K T T R G P T N V C I E Y A D S S F R L D S N C L S R P R I L A F P D V V S L V Q H Y V A S C T A D T R S D S P D P A P T P A L P M P K E D A P S D P A L P A P P P A T A V H L K L V Q P F V R R S S A R S L Q H L C R L V I N R L V A D V D C L P L P R R M A G Y L R Q Y P F Q L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes Recombinant protein. |
Target data
Function
SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. CIS is involved in the negative regulation of cytokines that signal through the JAK-STAT5 pathway such as erythropoietin, prolactin and interleukin 3 (IL3) receptor. Inhibits STAT5 trans-activation by suppressing its tyrosine phosphorylation. May be a substrate-recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (By similarity).
Alternative names
G18, CISH, Cytokine-inducible SH2-containing protein, CIS, CIS-1, Protein G18, Suppressor of cytokine signaling, SOCS
Recommended products
Recombinant Human CISH/CIS protein is a Human Full Length protein, in the 1 to 258 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- Q9NSE2-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.3% Glutathione
Sequence info
Amino acid sequence
- M V L C V Q G P R P L L A V E R T G Q R P L W A P S L E L P K P V M Q P L P A G A F L E E V A E G T P A Q T E S E P K V L D P E E D L L C I A K T F S Y L R E S G W Y W G S I T A S E A R Q H L Q K M P E G T F L V R D S T H P S Y L F T L S V K T T R G P T N V C I E Y A D S S F R L D S N C L S R P R I L A F P D V V S L V Q H Y V A S C T A D T R S D S P D P A P T P A L P M P K E D A P S D P A L P A P P P A T A V H L K L V Q P F V R R S S A R S L Q H L C R L V I N R L V A D V D C L P L P R R M A G Y L R Q Y P F Q L
- Accession
- Q9NSE2
- Protein length
- Full Length
- Predicted molecular weight
- 54.49 kDa
- Amino acids
- 1 to 258
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. CIS is involved in the negative regulation of cytokines that signal through the JAK-STAT5 pathway such as erythropoietin, prolactin and interleukin 3 (IL3) receptor. Inhibits STAT5 trans-activation by suppressing its tyrosine phosphorylation. May be a substrate-recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (By similarity).
- Post-translational modifications
Association with EPOR may target the protein for proteolysis by the ubiquitin-dependent proteasome pathway. CIS is mainly monubiquitinated (37 kDa form) but may also exist in a polyubiquitinated form (45 kDa).
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as CISH.
Product promise
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1 product image
SDS-PAGE - Recombinant Human CISH/CIS protein (ab117057)
12.5% SDS-PAGE showing ab117057 at approximately 54.49 kDa.
Stained with Coomassie Blue.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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