Recombinant Human Citrate synthetase protein is a Human Full Length protein, in the 28 to 466 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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- The EMBO journal 42:e1126472023L-arginine homeostasis governs adult neural stem cell activation by modulating energy metabolism in vivo.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMingyue Xu,Ye Guo,Min Wang,Xing Luo,Xuning Shen,Zhimin Li,Lei Wang,Weixiang GuoPubMed 36740997
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S A S S T N L K D I L A D L I P K E Q A R I K T F R Q Q H G K T V V G Q I T V D M M Y G G M R G M K G L V Y E T S V L D P D E G I R F R G F S I P E C Q K L L P K A K G G E E P L P E G L F W L L V T G H I P T E E Q V S W L S K E W A K R A A L P S H V V T M L D N F P T N L H P M S Q L S A A V T A L N S E S N F A R A Y A Q G I S R T K Y W E L I Y E D S M D L I A K L P C V A A K I Y R N L Y R E G S G I G A I D S N L D W S H N F T N M L G Y T D H Q F T E L T R L Y L T I H S D H E G G N V S A H T S H L V G S A L S D P Y L S F A A A M N G L A G P L H G L A N Q E V L V W L T Q L Q K E V G K D V S D E K L R D Y I W N T L N S G R V V P G Y G H A V L R K T D P R Y T C Q R E F A L K H L P N D P M F K L V A Q L Y K I V P N V L L E Q G K A K N P W P N V D A H S G V L L Q Y Y G M T E M N Y Y T V L F G V S R A L G V L A Q L I W S R A L G F P L E R P K S M S T E G L M K F V D S K S G
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Key enzyme of the Krebs tricarboxylic acid cycle which catalyzes the synthesis of citrate from acetyl coenzyme A and oxaloacetate.
Alternative names
Citrate (Si)-synthase, CS
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Recombinant Human Citrate synthetase protein is a Human Full Length protein, in the 28 to 466 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- O75390-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S A S S T N L K D I L A D L I P K E Q A R I K T F R Q Q H G K T V V G Q I T V D M M Y G G M R G M K G L V Y E T S V L D P D E G I R F R G F S I P E C Q K L L P K A K G G E E P L P E G L F W L L V T G H I P T E E Q V S W L S K E W A K R A A L P S H V V T M L D N F P T N L H P M S Q L S A A V T A L N S E S N F A R A Y A Q G I S R T K Y W E L I Y E D S M D L I A K L P C V A A K I Y R N L Y R E G S G I G A I D S N L D W S H N F T N M L G Y T D H Q F T E L T R L Y L T I H S D H E G G N V S A H T S H L V G S A L S D P Y L S F A A A M N G L A G P L H G L A N Q E V L V W L T Q L Q K E V G K D V S D E K L R D Y I W N T L N S G R V V P G Y G H A V L R K T D P R Y T C Q R E F A L K H L P N D P M F K L V A Q L Y K I V P N V L L E Q G K A K N P W P N V D A H S G V L L Q Y Y G M T E M N Y Y T V L F G V S R A L G V L A Q L I W S R A L G F P L E R P K S M S T E G L M K F V D S K S G
- Accession
- O75390
- Protein length
- Full Length
- Predicted molecular weight
- 51.4 kDa
- Amino acids
- 28 to 466
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab183252 ass purified using conventional chromatography techniques.
General info
- Function
Key enzyme of the Krebs tricarboxylic acid cycle which catalyzes the synthesis of citrate from acetyl coenzyme A and oxaloacetate.
- Sequence similarities
Belongs to the citrate synthase family.
- Post-translational modifications
Methylated (PubMed:28391595, PubMed:28887308). Trimethylation at Lys-395 by CSKMT decreases citrate synthase activity (PubMed:28887308).
- Subcellular localisation
- Mitochondrion matrix
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Citrate synthetase also known as citrate synthase is an important enzyme in the tricarboxylic acid cycle. It catalyzes the condensation of acetyl-CoA and oxaloacetate to form citrate and CoA. The enzyme weighs around 49 kilodaltons. It is expressed prominently in the mitochondria of eukaryotic cells where it initiates the Krebs cycle by providing citrate to be further processed. Citrate synthetase is an important point of control within this metabolic cycle.
- Biological function summary
Citrate synthetase plays a central role in energy production by converting oxaloacetate and acetyl-CoA into citrate. This enzyme is not known to be part of any larger complex but associates with downstream enzymes such as aconitase in the metabolic pathway. Its activity is essential for cellular respiration impacting the overall metabolic rate of the organism. The concentration of citrate produced serves as a checkpoint both for the continuation of the Krebs cycle and for feedback inhibition of glycolysis.
- Pathways
Citrate synthetase is integral to the Krebs cycle and the related oxidative phosphorylation pathway. These pathways are essential for efficient ATP production. Citrate synthetase works closely with enzymes like isocitrate dehydrogenase and alpha-ketoglutarate dehydrogenase within the Krebs cycle. Through these interactions citrate synthetase ensures the proper flow of carbon through the cycle impacting ATP yield and cellular energy homeostasis.
- Associated diseases and disorders
Citrate synthetase's function can influence metabolic diseases like diabetes and mitochondrial disorders. Alterations in its activity may contribute to the dysregulation of glucose metabolism seen in diabetes affecting enzymes like glucose transporter 4 (GLUT4). In mitochondrial disorders changes in its normal activity can cause energy production deficiencies influencing proteins such as cytochrome c which is critical in the electron transport chain. Understanding these interactions can help develop therapeutic strategies targeting metabolic pathways.
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SDS-PAGE - Recombinant Human Citrate synthetase protein (ab183252)
15% SDS PAGE analysis of ab183252 (3μg).
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