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AB104024

Recombinant human CK1 epsilon protein

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Recombinant human CK1 epsilon protein is a Human Full Length protein, in the 1 to 416 aa range, expressed in Baculovirus infected Sf9 cells, with >75%, suitable for SDS-PAGE, FuncS.

View Alternative Names

Casein kinase I isoform epsilon, CKI-epsilon, CKIe, CSNK1E

4 Images
Functional Studies - Recombinant human CK1 epsilon protein (AB104024)
  • FuncS

Unknown

Functional Studies - Recombinant human CK1 epsilon protein (AB104024)

The Specific activity of ab104024 was determined to be 46 nmol/min/mg.

Functional Studies - Recombinant human CK1 epsilon protein (AB104024)
  • FuncS

Unknown

Functional Studies - Recombinant human CK1 epsilon protein (AB104024)

The specific activity of CK1 epsilon (ab104024) was determined to be 39 nmol/min/mg as per activity assay protocol

SDS-PAGE - Recombinant human CK1 epsilon protein (AB104024)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human CK1 epsilon protein (AB104024)

SDS-PAGE showing ab104024 at approximately 72kDa.

SDS-PAGE - Recombinant human CK1 epsilon protein (AB104024)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human CK1 epsilon protein (AB104024)

SDS PAGE analysis of ab104024

Key facts

Purity

>75% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

The Specific activity of ab104024 was determined to be 46 nmol/min/mg.

Accession

P49674

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.307% Glutathione, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00292% EDTA, 0.00174% PMSF

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Secondary Antibodies

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Primary Antibodies

AB302638

Anti-CK1 epsilon antibody [1/Casein Kinase Iε]

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Recombinant Human CRBN + DDB1 + CUL-4A + RBX1 protein (DDDDK tag N-Terminus, His tag N-Terminus)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

ab91090 (Cow Casein full length protein) can be utilized as a substrate for assessing Kinase activity
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Sequence info

[{"linker":null,"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"72 kDa","actualMolecularWeight":null,"aminoAcidEnd":416,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P49674","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Specifications

Form

Liquid

Additional notes

Purity was determined to be >75% by densitometry. Affinity purified.

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General info

Function

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates (Probable). Participates in Wnt signaling (PubMed : 12556519, PubMed : 23413191). Phosphorylates DVL1 (PubMed : 12556519). Phosphorylates DVL2 (PubMed : 23413191). Phosphorylates NEDD9/HEF1 (By similarity). Central component of the circadian clock (PubMed : 16790549). In balance with PP1, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation (PubMed : 15917222, PubMed : 16790549). Controls PER1 and PER2 nuclear transport and degradation (By similarity). Inhibits cytokine-induced granuloytic differentiation (PubMed : 15070676).

Sequence similarities

Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.

Post-translational modifications

Autophosphorylated. Partially dephosphorylated by PPP5C. May be dephosphorylated by PP1.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates (Probable). Participates in Wnt signaling (PubMed : 12556519, PubMed : 23413191). Phosphorylates DVL1 (PubMed : 12556519). Phosphorylates DVL2 (PubMed : 23413191). Phosphorylates NEDD9/HEF1 (By similarity). Central component of the circadian clock (PubMed : 16790549). In balance with PP1, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation (PubMed : 15917222, PubMed : 16790549). Controls PER1 and PER2 nuclear transport and degradation (By similarity). Inhibits cytokine-induced granuloytic differentiation (PubMed : 15070676).
See full target information Casein kinase I isoform epsilon
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Product promise

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