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AB130049

Recombinant Human Clathrin light chain B protein (His tag N-Terminus)

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Recombinant Human Clathrin light chain B protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 211 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

Clathrin light chain B, Lcb, CLTB

1 Images
SDS-PAGE - Recombinant Human Clathrin light chain B protein (His tag N-Terminus) (AB130049)
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SDS-PAGE - Recombinant Human Clathrin light chain B protein (His tag N-Terminus) (AB130049)

15% SDS-PAGE analysis of 3 µg of Human Clathrin light chain B protein (ab130049) Note : Molecular weight on SDS-PAGE will appear higher than predicted.

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

P09497

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.004% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMADDFGFFSSSESGAPEAAEEDPAAAFLAQQESEIAGIENDEGFGAPAGSHAAPAQPGPTSGAGSEDMGTTVNGDVFQEANGPADGYAAIAQADRLTQEPESIRKWREEQRKRLQELDAASKVTEQEWREKAKKDLEEWNQRQSEQVEKNKINNRASEEAFVKESKEETPGTEWEKVAQLCDFNPKSSKQCKDVSRLRSVLMSLKQTPLSR","proteinLength":"Full Length","predictedMolecularWeight":"25.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":211,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P09497","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Clathrin light chain B often known as CLTB is a component of the clathrin triskelion a structure important in endocytosis. It has a molecular mass of about 25 kDa and is ubiquitously expressed in various tissues with abundant presence in neuronal and non-neuronal cells. The clathrin triskelion consists of three heavy chains and three light chains including the clathrin light chains A and B forming a scaffold that facilitates the formation of clathrin-coated vesicles.
Biological function summary

Clathrin light chain B plays an essential role in the assembly of clathrin-coated vesicles a process fundamental for receptor-mediated endocytosis. It is part of the clathrin complex interacting with adaptor protein complexes and helping them to coat and stabilize formed vesicles. This enables the transport of molecules into the cell recycling of receptors and intracellular signaling processes. The light chains are important for regulating the lattice structure of clathrin influencing the vesicle size and curvature.

Pathways

Clathrin light chain B is integral in endocytic and vesicular trafficking pathways. It is associated with the AP2 adaptor complex which is important for selecting cargo for clathrin-mediated endocytosis. Additionally the protein intersects with the regulated secretory pathway interacting with proteins such as dynamin and amphiphysin which assist in membrane scission during vesicle formation.

Dysregulation of clathrin light chain B appears linked to neurodegenerative disorders like Alzheimer's disease and Huntington's disease. Abnormalities in clathrin-mediated endocytosis disrupt neuronal homeostasis. In these conditions misprocessing of amyloid precursor protein involves clathrin complexes including CLTB signifying its role. Collaborators in this process include proteins like presenilin-1 and beta-secretase which are involved in the generation of amyloid-beta peptides contributing to plaque formation.
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Specifications

Form

Liquid

Additional notes

ab130049 was purified using conventional chromatography techniques.

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General info

Function

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles.

Sequence similarities

Belongs to the clathrin light chain family.

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Product protocols

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Target data

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles.
See full target information Clathrin light chain B
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Primary Antibodies

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