Recombinant Human Clathrin light chain protein is a Human Full Length protein, in the 1 to 218 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Images
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S H M A E L D P F G A P A G A P G G P A L G N G V A G A G E E D P A A A F L A Q Q E S E I A G I E N D E A F A I L D G G A P G P Q P H G E P P G G P D A V D G V M N G E Y Y Q E S N G P T D S Y A A I S Q V D R L Q S E P E S I R K W R E E Q M E R L E A L D A N S R K Q E A E W K E K A I K E L E E W Y A R Q D E Q L Q K T K A N N R A A E E A F V N D I D E S S P G T E W E R V A R L C D F N P K S S K Q A K D V S R M R S V L I S L K Q A P L V H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Acts as a component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge (PubMed:15858577, PubMed:21297582).
Alternative names
Clathrin light chain A, Lca, CLTA
Recommended products
Recombinant Human Clathrin light chain protein is a Human Full Length protein, in the 1 to 218 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- P09496-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S H M A E L D P F G A P A G A P G G P A L G N G V A G A G E E D P A A A F L A Q Q E S E I A G I E N D E A F A I L D G G A P G P Q P H G E P P G G P D A V D G V M N G E Y Y Q E S N G P T D S Y A A I S Q V D R L Q S E P E S I R K W R E E Q M E R L E A L D A N S R K Q E A E W K E K A I K E L E E W Y A R Q D E Q L Q K T K A N N R A A E E A F V N D I D E S S P G T E W E R V A R L C D F N P K S S K Q A K D V S R M R S V L I S L K Q A P L V H
- Accession
- P09496
- Protein length
- Full Length
- Predicted molecular weight
- 26.2 kDa
- Amino acids
- 1 to 218
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab126693 is purified by conventional chromatography techniques.
General info
- Function
Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Acts as a component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge (PubMed:15858577, PubMed:21297582).
- Sequence similarities
Belongs to the clathrin light chain family.
- Subcellular localisation
- Cytoskeleton, Spindle
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Clathrin light chain also known as clathrin LCa and LCb is a low-molecular-weight component of the clathrin protein complex. It plays a mechanical role in the processes that drive clathrin-mediated endocytosis. Each clathrin triskelion the basic unit of the clathrin coat comprises three clathrin heavy chains and three clathrin light chains. The mass of clathrin light chain is approximately 25-29 kDa. This protein is expressed in various tissues throughout the body with notable presence in the central nervous system and liver.
- Biological function summary
Clathrin light chain modulates the lattice dynamics by interacting with clathrin heavy chains in a clathrin protein complex. This interaction supports the stability and assembly of the clathrin lattice affecting the efficiency and specificity of endocytic vesicle formation. Clathrin light chain acts in concert with other accessory proteins to regulate clathrin coat assembly and disassembly playing a dynamic role in cellular trafficking processes.
- Pathways
The clathrin light chain participates in essential cellular pathways such as the clathrin-mediated endocytosis and synaptic vesicle recycling pathways. These pathways facilitate the internalization and recycling of membrane proteins and lipids ensuring cellular homeostasis. In these processes the clathrin light chain works closely with related proteins such as dynamin and adaptin to control vesicle scission and cargo selection.
- Associated diseases and disorders
Abnormalities in clathrin light chain function connect to neurodegenerative disorders like Huntington's disease and certain types of cancers. In Huntington's disease disrupted endocytic pathways that involve clathrin light chain impact cellular communication and nutrient uptake. Cancer cells may exhibit altered expression or function of clathrin light chain affecting pathways important for cell proliferation and survival. The clathrin light chain can interact with the huntingtin protein in these contexts influencing disease progression and cellular dynamics.
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SDS-PAGE - Recombinant Human Clathrin light chain protein (ab126693)
15% SDS-PAGE analysis of 3 μg of ab126693 (Clathrin light chain).
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