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AB112383

Recombinant Human Claudin 2 protein

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Recombinant Human Claudin 2 protein is a Human Fragment protein, in the 29 to 80 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

PSEC0059, SP82, UNQ705/PRO1356, CLDN2, Claudin-2

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SDS-PAGE - Recombinant Human Claudin 2 protein (AB112383)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Claudin 2 protein (AB112383)

ab112383 analysed by 12.5% SDS-PAGE and stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, WB, SDS-PAGE

applications

Biologically active

No

Accession

P57739

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product is useful for Antibody Production and Protein Array.
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Sequence info

[{"sequence":"SWKTSSYVGASIVTAVGFSKGLWMECATHSTGITQCDIYSTLLGLPADIQAA","proteinLength":"Fragment","predictedMolecularWeight":"31.35 kDa","actualMolecularWeight":null,"aminoAcidEnd":80,"aminoAcidStart":29,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P57739","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Claudin-2 also known as CLDN2 is a protein that plays an important role in the formation and function of tight junctions between epithelial and endothelial cells. This structural protein is approximately 22 kDa in mass. It is mainly expressed in tissues with high transepithelial transport such as the kidney and intestine. As a member of the claudins Claudin-2 contributes to the paracellular transport of small ions and water distinguishing itself from other tight junction proteins.
Biological function summary

Claudin-2 facilitates the creation of ion-selective pores in the tight junction complexes allowing it to regulate paracellular permeability and maintain ion balance. It is part of the larger tight junction complex that includes proteins like occludin and zona occludens. Through these interactions Claudin-2 supports the maintenance of epithelial barrier function which is essential for controlled passage of ions and molecules in tissues.

Pathways

Claudin-2 participates in the signaling pathways related to epithelial cell polarity and transepithelial transport. It is significantly involved in the MAPK and PI3K-Akt signaling pathways which are important for cell growth and survival. It includes interactions with related proteins such as Claudin-1 and Claudin-4 which modulate the structure and integrity of tight junctions in response to various cellular signals.

An imbalance or dysregulation of Claudin-2 expression can contribute to pathological conditions like inflammatory bowel disease (IBD) and hypercalciuria. Defects in Claudin-2 disrupt barrier function increasing permeability and often correlating with disease progression. Additionally proteins like ZO-1 and ZO-2 are associated with Claudin-2 in these disorders as they together influence the dynamics and composition of tight junctions under pathophysiological conditions.
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Specifications

Form

Liquid

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General info

Function

Forms paracellular channels : polymerizes in tight junction strands with cation- and water-selective channels through the strands, conveying epithelial permeability in a process known as paracellular tight junction permeability (PubMed : 20460438, PubMed : 36008380). In intestinal epithelium, allows for sodium and water fluxes from the peritoneal side to the lumen of the intestine to regulate nutrient absorption and clear enteric pathogens as part of mucosal immune response (By similarity). In kidney, allows passive sodium and calcium reabsorption across proximal tubules from the lumen back to the bloodstream (By similarity). In the hepatobiliary tract, allows paracellular water and cation fluxes in the hepatic perivenous areas and biliary epithelium to generate bile flow and maintain osmotic gradients (By similarity).

Sequence similarities

Belongs to the claudin family.

Post-translational modifications

The disulfide bond is necessary for pore formation, but is not required for correct protein trafficking.

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Product protocols

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Target data

Forms paracellular channels : polymerizes in tight junction strands with cation- and water-selective channels through the strands, conveying epithelial permeability in a process known as paracellular tight junction permeability (PubMed : 20460438, PubMed : 36008380). In intestinal epithelium, allows for sodium and water fluxes from the peritoneal side to the lumen of the intestine to regulate nutrient absorption and clear enteric pathogens as part of mucosal immune response (By similarity). In kidney, allows passive sodium and calcium reabsorption across proximal tubules from the lumen back to the bloodstream (By similarity). In the hepatobiliary tract, allows paracellular water and cation fluxes in the hepatic perivenous areas and biliary epithelium to generate bile flow and maintain osmotic gradients (By similarity).
See full target information CLDN2
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Complementary Products

Primary Antibodies

AB15098

Anti-Claudin 1 antibody

Western blot - Anti-Claudin 1 antibody (AB15098)

  • 4
  • 21
Primary Antibodies

AB216327

Anti-Occludin antibody [EPR20992]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] (AB216327)

  • 4
  • 7
Primary Antibodies

AB131259

Anti-Claudin 5 antibody [EPR7583]

RabMAb|Recombinant|20ul selling size

Western blot - Anti-Claudin 5 antibody [EPR7583] (AB131259)

  • 4
  • 7
Primary Antibodies

AB221547

Anti-ZO1 tight junction protein antibody [EPR19945-296]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-296] (AB221547)

  • 5
  • 6

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