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Recombinant Human Claudin 4 (Active) protein is a Human Full Length protein, in the 1 to 209 aa range, expressed in HEK 293, with >95% purity and < 1 EU/µg endotoxin level.

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Key facts

Purity
>95% HPLC
Endotoxin level
< 1 EU/µg
Expression system
HEK 293 cells
Tags
Tag free
Biologically active
Yes

Amino acid sequence

M A S M G L Q V M G I A L A V L G W L A V M L C C A L P M W R V T A F I G S N I V T S Q T I W E G L W M N C V V Q S T G Q M Q C K V Y D S L L A L P Q D L Q A A R A L V I I S I I V A A L G V L L S V V G G K C T N C L E D E S A K A K T M I V A G V V F L L A G L M V I V P V S W T A H N I I Q D F Y N P L V A S G Q K R E M G A S L Y V G W A A S G L L L L G G G L L C C N C P P R T D K P Y S A K Y S A A R S A A A S N Y V

Target data

Function

Can associate with other claudins to regulate tight junction structural and functional strand dynamics (PubMed:35773259, PubMed:36008380). May coassemble with CLDN8 into tight junction strands containing anion-selective channels that convey paracellular chloride permeability in renal collecting ducts (By similarity) (PubMed:36008380). May integrate into CLDN3 strands to modulate localized tight junction barrier properties (PubMed:35773259, PubMed:36008380). May disrupt strand assembly of channel-forming CLDN2 and CLDN15 and inhibit cation conductance (PubMed:35773259, PubMed:36008380). Cannot form tight junction strands on its own (PubMed:35773259, PubMed:36008380).

Alternative names

Recommended products

Recombinant Human Claudin 4 (Active) protein is a Human Full Length protein, in the 1 to 209 aa range, expressed in HEK 293, with >95% purity and < 1 EU/µg endotoxin level.

Key facts

Purity
>95% HPLC
Endotoxin level
< 1 EU/µg
Expression system
HEK 293 cells
Biological activity
Immobilized Human Claudin 4 VLP at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Claudin 4 Antibody, hFc Tag with the EC50 of 18.4ng/ml determined by ELISA.
Accession
O14493-1
Animal free
No
Carrier free
Yes
Species
Human
Concentration
Loading...
Storage buffer

Constituents: 99.99% PBS

Sequence info

Amino acid sequence

M A S M G L Q V M G I A L A V L G W L A V M L C C A L P M W R V T A F I G S N I V T S Q T I W E G L W M N C V V Q S T G Q M Q C K V Y D S L L A L P Q D L Q A A R A L V I I S I I V A A L G V L L S V V G G K C T N C L E D E S A K A K T M I V A G V V F L L A G L M V I V P V S W T A H N I I Q D F Y N P L V A S G Q K R E M G A S L Y V G W A A S G L L L L G G G L L C C N C P P R T D K P Y S A K Y S A A R S A A A S N Y V
Accession
O14493
Protein length
Full Length
Predicted molecular weight
23.31 kDa
Amino acids
1 to 209
Nature
Recombinant

Specifications

Form
Liquid

General info

Function

Can associate with other claudins to regulate tight junction structural and functional strand dynamics (PubMed:35773259, PubMed:36008380). May coassemble with CLDN8 into tight junction strands containing anion-selective channels that convey paracellular chloride permeability in renal collecting ducts (By similarity) (PubMed:36008380). May integrate into CLDN3 strands to modulate localized tight junction barrier properties (PubMed:35773259, PubMed:36008380). May disrupt strand assembly of channel-forming CLDN2 and CLDN15 and inhibit cation conductance (PubMed:35773259, PubMed:36008380). Cannot form tight junction strands on its own (PubMed:35773259, PubMed:36008380).

Sequence similarities

Belongs to the claudin family.

Post-translational modifications

Phosphorylated. Phosphorylation by EPHA2 is stimulated by EFNA1 and alters interaction with TJP1.

Storage

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Claudin-4 also known as CLDN4 is a protein essential for tight junctions which are critical in maintaining the permeability barrier of epithelial cells. The protein has a molecular mass of approximately 22 kDa. Claudin-4 expresses notably in epithelial cells of various tissues including the kidney lung and gastrointestinal tract. In addition to its presence in normal tissues claudin-4 can also exhibit heightened expression in certain cancerous tissues making it a subject of interest in oncology research.

Biological function summary

Claudin-4 plays a significant role in paracellular transport and cell polarity. By assembling into complexes with other claudins and tight junction proteins claudin-4 regulates the selective permeability and homeostasis across cell layers. It functions as a component of tight junction strands contributing to their barrier properties. Interaction with proteins such as occludin and ZO-1 further defines its role in tight junction structure and function.

Pathways

Claudin-4 participates in critical biological processes such as epithelial cell signaling and apoptosis regulation. It interfaces with the epithelial-to-mesenchymal transition (EMT) pathway where claudin-4 expression influences cellular adhesion and motility. Claudin-4 also interacts with related claudin proteins in these pathways contributing to structural arrangement and pathway signaling precision.

Associated diseases and disorders

Claudin-4 has associations with several types of cancers including pancreatic and ovarian cancer. Its consistent overexpression in these conditions suggests its involvement in tumorigenesis and metastasis. In the context of ovarian cancer claudin-4 may interact with proteins such as mesothelin which plays a role in cell adhesion and tumor progression. Investigating claudin-4's involvement in cancer can provide insights into potential therapeutic targets and diagnostic markers.

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