Recombinant human Claudin 4 protein - Active (His tag) is a Human Full Length protein, in the 1 to 209 aa range, expressed in Mammalian, < 1 EU/µg endotoxin level and suitable for WB, FuncS.
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Key facts
- Endotoxin level
< 1 EU/µg
- Expression system
Mammalian
- Tags
His tag C-Terminus
- Applications
WB, FuncS
- Biologically active
Yes
Amino acid sequence
M A S M G L Q V M G I A L A V L G W L A V M L C C A L P M W R V T A F I G S N I V T S Q T I W E G L W M N C V V Q S T G Q M Q C K V Y D S L L A L P Q D L Q A A R A L V I I S I I V A A L G V L L S V V G G K C T N C L E D E S A K A K T M I V A G V V F L L A G L M V I V P V S W T A H N I I Q D F Y N P L V A S G Q K R E M G A S L Y V G W A A S G L L L L G G G L L C C N C P P R T D K P Y S A K Y S A A R S A A A S N Y V
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Channel-forming tight junction protein that mediates paracellular chloride transport in the kidney. Plays a critical role in the paracellular reabsorption of filtered chloride in the kidney collecting ducts. Claudins play a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
Alternative names
Claudin-4, Clostridium perfringens enterotoxin receptor, Williams-Beuren syndrome chromosomal region 8 protein, CPE-R, CPE-receptor, WBSCR8, CPETR1, CPER, CLDN4
Recommended products
Recombinant human Claudin 4 protein - Active (His tag) is a Human Full Length protein, in the 1 to 209 aa range, expressed in Mammalian, < 1 EU/µg endotoxin level and suitable for WB, FuncS.
Alternative names
Claudin-4, Clostridium perfringens enterotoxin receptor, Williams-Beuren syndrome chromosomal region 8 protein, CPE-R, CPE-receptor, WBSCR8, CPETR1, CPER, CLDN4
Key facts
- Endotoxin level
< 1 EU/µg
- Expression system
Mammalian
- Tags
His tag C-Terminus
- Applications
WB, FuncS
- Biologically active
Yes
- Biological activity
Measured by its binding ability in a functional ELISA. AB314531 at 5 μg/mL can bind an Anti-CLDN4 recombinant antibody, the EC50 is 29.56-50.75 ng/mL.
- Accession
- O14493-1
- Animal free
Yes
- Species
Human
- Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%.
- Concentration
- Storage buffer
pH: 7.4 - 8
Constituents: 6% Trehalose, 0.14% PBS
Sequence info
Amino acid sequence
M A S M G L Q V M G I A L A V L G W L A V M L C C A L P M W R V T A F I G S N I V T S Q T I W E G L W M N C V V Q S T G Q M Q C K V Y D S L L A L P Q D L Q A A R A L V I I S I I V A A L G V L L S V V G G K C T N C L E D E S A K A K T M I V A G V V F L L A G L M V I V P V S W T A H N I I Q D F Y N P L V A S G Q K R E M G A S L Y V G W A A S G L L L L G G G L L C C N C P P R T D K P Y S A K Y S A A R S A A A S N Y V
- Accession
- Protein length
Full Length
- Predicted molecular weight
23.4 kDa
- Amino acids
1 to 209
- Nature
Recombinant
- Tags
His tag C-Terminus
Specifications
- Form
Lyophilized
- Additional notes
Purification technique - Centrifugation.
General info
- Function
Channel-forming tight junction protein that mediates paracellular chloride transport in the kidney. Plays a critical role in the paracellular reabsorption of filtered chloride in the kidney collecting ducts. Claudins play a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
- Sequence similarities
Belongs to the claudin family.
- Post-translational modifications
Phosphorylated. Phosphorylation by EPHA2 is stimulated by EFNA1 and alters interaction with TJP1.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C, -80°C
- Aliquoting information
Add glycerol to a final volume of 50% for extra stability and aliquot
- Storage information
The product can be stored for up to 12 months
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20degC/-80degC. Our default final concentration of glycerol is 50%.
Supplementary info
- Activity summary
Claudin-4 also known as CLDN4 is a protein essential for tight junctions which are critical in maintaining the permeability barrier of epithelial cells. The protein has a molecular mass of approximately 22 kDa. Claudin-4 expresses notably in epithelial cells of various tissues including the kidney lung and gastrointestinal tract. In addition to its presence in normal tissues claudin-4 can also exhibit heightened expression in certain cancerous tissues making it a subject of interest in oncology research.
- Biological function summary
Claudin-4 plays a significant role in paracellular transport and cell polarity. By assembling into complexes with other claudins and tight junction proteins claudin-4 regulates the selective permeability and homeostasis across cell layers. It functions as a component of tight junction strands contributing to their barrier properties. Interaction with proteins such as occludin and ZO-1 further defines its role in tight junction structure and function.
- Pathways
Claudin-4 participates in critical biological processes such as epithelial cell signaling and apoptosis regulation. It interfaces with the epithelial-to-mesenchymal transition (EMT) pathway where claudin-4 expression influences cellular adhesion and motility. Claudin-4 also interacts with related claudin proteins in these pathways contributing to structural arrangement and pathway signaling precision.
- Associated diseases and disorders
Claudin-4 has associations with several types of cancers including pancreatic and ovarian cancer. Its consistent overexpression in these conditions suggests its involvement in tumorigenesis and metastasis. In the context of ovarian cancer claudin-4 may interact with proteins such as mesothelin which plays a role in cell adhesion and tumor progression. Investigating claudin-4's involvement in cancer can provide insights into potential therapeutic targets and diagnostic markers.
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2 product images
Western blot - Recombinant human Claudin 4 protein - Active (His tag) (ab314531)
All lanes: mouse anti-6*His monoclonal antibody
All lanes: Western blot - Recombinant human Claudin 4 protein - Active (His tag) (ab314531)
Functional Studies - Recombinant human Claudin 4 protein - Active (His tag) (ab314531)
Measured by its binding ability in a functional ELISA. ab314531 at 5 μg/mL can bind an Anti-CLDN4 recombinant antibody, the EC50 is 29.56-50.75 ng/mL.
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