Recombinant human Claudin 4 protein - Active (His tag) is a Human Full Length protein, in the 1 to 209 aa range, expressed in Mammalian, < 1 EU/µg endotoxin level and suitable for WB, FuncS.
Images
Key facts
- Endotoxin level
- < 1 EU/µg
- Expression system
- Mammalian
- Tags
- His tag C-Terminus
- Applications
- WB, FuncS
- Biologically active
- Yes
Amino acid sequence
M A S M G L Q V M G I A L A V L G W L A V M L C C A L P M W R V T A F I G S N I V T S Q T I W E G L W M N C V V Q S T G Q M Q C K V Y D S L L A L P Q D L Q A A R A L V I I S I I V A A L G V L L S V V G G K C T N C L E D E S A K A K T M I V A G V V F L L A G L M V I V P V S W T A H N I I Q D F Y N P L V A S G Q K R E M G A S L Y V G W A A S G L L L L G G G L L C C N C P P R T D K P Y S A K Y S A A R S A A A S N Y V
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Can associate with other claudins to regulate tight junction structural and functional strand dynamics (PubMed:35773259, PubMed:36008380). May coassemble with CLDN8 into tight junction strands containing anion-selective channels that convey paracellular chloride permeability in renal collecting ducts (By similarity) (PubMed:36008380). May integrate into CLDN3 strands to modulate localized tight junction barrier properties (PubMed:35773259, PubMed:36008380). May disrupt strand assembly of channel-forming CLDN2 and CLDN15 and inhibit cation conductance (PubMed:35773259, PubMed:36008380). Cannot form tight junction strands on its own (PubMed:35773259, PubMed:36008380).
Alternative names
CPER, CPETR1, WBSCR8, CLDN4, Claudin-4, Clostridium perfringens enterotoxin receptor, Williams-Beuren syndrome chromosomal region 8 protein, CPE-R, CPE-receptor
Recommended products
Recombinant human Claudin 4 protein - Active (His tag) is a Human Full Length protein, in the 1 to 209 aa range, expressed in Mammalian, < 1 EU/µg endotoxin level and suitable for WB, FuncS.
Key facts
- Endotoxin level
- < 1 EU/µg
- Expression system
- Mammalian
- Tags
- His tag C-Terminus
- Applications
- WB, FuncS
- Biologically active
- Yes
- Biological activity
- Measured by its binding ability in a functional ELISA. AB314531 at 5 μg/mL can bind an Anti-CLDN4 recombinant antibody, the EC50 is 29.56-50.75 ng/mL.
- Accession
- O14493-1
- Animal free
- Yes
- Species
- Human
- Reconstitution
- We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%.
- Concentration
- Storage buffer
pH: 7.4 - 8
Constituents: 6% Trehalose, 0.14% PBS
Sequence info
Amino acid sequence
- M A S M G L Q V M G I A L A V L G W L A V M L C C A L P M W R V T A F I G S N I V T S Q T I W E G L W M N C V V Q S T G Q M Q C K V Y D S L L A L P Q D L Q A A R A L V I I S I I V A A L G V L L S V V G G K C T N C L E D E S A K A K T M I V A G V V F L L A G L M V I V P V S W T A H N I I Q D F Y N P L V A S G Q K R E M G A S L Y V G W A A S G L L L L G G G L L C C N C P P R T D K P Y S A K Y S A A R S A A A S N Y V
- Accession
- O14493
- Protein length
- Full Length
- Predicted molecular weight
- 23.4 kDa
- Amino acids
- 1 to 209
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
- Additional notes
Purification technique - Centrifugation.
General info
- Function
Can associate with other claudins to regulate tight junction structural and functional strand dynamics (PubMed:35773259, PubMed:36008380). May coassemble with CLDN8 into tight junction strands containing anion-selective channels that convey paracellular chloride permeability in renal collecting ducts (By similarity) (PubMed:36008380). May integrate into CLDN3 strands to modulate localized tight junction barrier properties (PubMed:35773259, PubMed:36008380). May disrupt strand assembly of channel-forming CLDN2 and CLDN15 and inhibit cation conductance (PubMed:35773259, PubMed:36008380). Cannot form tight junction strands on its own (PubMed:35773259, PubMed:36008380).
- Sequence similarities
Belongs to the claudin family.
- Post-translational modifications
Phosphorylated. Phosphorylation by EPHA2 is stimulated by EFNA1 and alters interaction with TJP1.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C, -80°C
- Aliquoting information
- Add glycerol to a final volume of 50% for extra stability and aliquot
- Storage information
- The product can be stored for up to 12 months
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
ab314531 is a VLP-CLDN4 protein complex.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Claudin-4 also known as CLDN4 is a protein essential for tight junctions which are critical in maintaining the permeability barrier of epithelial cells. The protein has a molecular mass of approximately 22 kDa. Claudin-4 expresses notably in epithelial cells of various tissues including the kidney lung and gastrointestinal tract. In addition to its presence in normal tissues claudin-4 can also exhibit heightened expression in certain cancerous tissues making it a subject of interest in oncology research.
- Biological function summary
Claudin-4 plays a significant role in paracellular transport and cell polarity. By assembling into complexes with other claudins and tight junction proteins claudin-4 regulates the selective permeability and homeostasis across cell layers. It functions as a component of tight junction strands contributing to their barrier properties. Interaction with proteins such as occludin and ZO-1 further defines its role in tight junction structure and function.
- Pathways
Claudin-4 participates in critical biological processes such as epithelial cell signaling and apoptosis regulation. It interfaces with the epithelial-to-mesenchymal transition (EMT) pathway where claudin-4 expression influences cellular adhesion and motility. Claudin-4 also interacts with related claudin proteins in these pathways contributing to structural arrangement and pathway signaling precision.
- Associated diseases and disorders
Claudin-4 has associations with several types of cancers including pancreatic and ovarian cancer. Its consistent overexpression in these conditions suggests its involvement in tumorigenesis and metastasis. In the context of ovarian cancer claudin-4 may interact with proteins such as mesothelin which plays a role in cell adhesion and tumor progression. Investigating claudin-4's involvement in cancer can provide insights into potential therapeutic targets and diagnostic markers.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Recombinant human Claudin 4 protein - Active (His tag) (ab314531)
All lanes: mouse anti-6*His monoclonal antibody
All lanes: Western blot - Recombinant human Claudin 4 protein - Active (His tag) (ab314531)
Functional Studies - Recombinant human Claudin 4 protein - Active (His tag) (ab314531)
Measured by its binding ability in a functional ELISA. ab314531 at 5 μg/mL can bind an Anti-CLDN4 recombinant antibody, the EC50 is 29.56-50.75 ng/mL.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com