Recombinant human Claudin 6 protein - Active (His tag) is a Human Full Length protein, in the 1 to 220 aa range, expressed in Mammalian, < 1 EU/µg endotoxin level and suitable for WB, FuncS.
Images
Key facts
- Endotoxin level
- < 1 EU/µg
- Expression system
- Mammalian
- Tags
- His tag C-Terminus
- Applications
- WB, FuncS
- Biologically active
- Yes
Amino acid sequence
M A S A G M Q I L G V V L T L L G W V N G L V S C A L P M W K V T A F I G N S I V V A Q V V W E G L W M S C V V Q S T G Q M Q C K V Y D S L L A L P Q D L Q A A R A L C V I A L L V A L F G L L V Y L A G A K C T T C V E E K D S K A R L V L T S G I V F V I S G V L T L I P V C W T A H A I I R D F Y N P L V A E A Q K R E L G A S L Y L G W A A S G L L L L G G G L L C C T C P S G G S Q G P S H Y M A R Y S T S A P A I S R G P S E Y P T K N Y V
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Plays a major role in tight junction-specific obliteration of the intercellular space. (Microbial infection) Acts as a receptor for hepatitis C virus (HCV) entry into hepatic cells.
Alternative names
UNQ757/PRO1488, CLDN6, Claudin-6, Skullin
Recommended products
Recombinant human Claudin 6 protein - Active (His tag) is a Human Full Length protein, in the 1 to 220 aa range, expressed in Mammalian, < 1 EU/µg endotoxin level and suitable for WB, FuncS.
Key facts
- Endotoxin level
- < 1 EU/µg
- Expression system
- Mammalian
- Tags
- His tag C-Terminus
- Applications
- WB, FuncS
- Biologically active
- Yes
- Biological activity
- Measured by its binding ability in a functional ELISA. AB314532 at 10 μg/mL can bind an Anti-CLDN6/9 recombinant antibody, the EC50 is 1.501-2.035 ng/mL
- Accession
- P56747-1
- Animal free
- Yes
- Species
- Human
- Reconstitution
- Reconstitute in water
- Concentration
- Storage buffer
pH: 7.4 - 8
Constituents: 6% Trehalose, 0.87% Sodium chloride, 0.24% Tris
Sequence info
Amino acid sequence
- M A S A G M Q I L G V V L T L L G W V N G L V S C A L P M W K V T A F I G N S I V V A Q V V W E G L W M S C V V Q S T G Q M Q C K V Y D S L L A L P Q D L Q A A R A L C V I A L L V A L F G L L V Y L A G A K C T T C V E E K D S K A R L V L T S G I V F V I S G V L T L I P V C W T A H A I I R D F Y N P L V A E A Q K R E L G A S L Y L G W A A S G L L L L G G G L L C C T C P S G G S Q G P S H Y M A R Y S T S A P A I S R G P S E Y P T K N Y V
- Accession
- P56747
- Protein length
- Full Length
- Predicted molecular weight
- 25.1 kDa
- Amino acids
- 1 to 220
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
- Additional notes
Purification technique - Centrifugation.
General info
- Function
Plays a major role in tight junction-specific obliteration of the intercellular space.
- Sequence similarities
Belongs to the claudin family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C, -80°C
- Appropriate long-term storage conditions
- -20°C, -80°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20degC/-80degC. Our default final concentration of glycerol is 50%.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Claudin 6 also known as CLDN6 is a transmembrane protein belonging to the claudin family. This family is critical in forming tight junctions between cells which regulate the paracellular barrier functions of epithelia. The molecular mass of claudin 6 is approximately 23 kDa. Researchers have observed its expression mainly in embryonic tissues and certain cancer types. In normal adult tissues it shows limited expression indicating a specific role during early developmental stages.
- Biological function summary
Claudin 6 participates in the formation and maintenance of tight junctions in epithelial cells. It is part of a larger complex that includes multiple claudins and other tight junction proteins like occludin and zonula occludens-1. This complex maintains cellular polarity and controls the flow of ions and molecules between cells which is essential for tissue homeostasis. Its expression pattern suggests a role in cell differentiation and proliferation during embryogenesis and tumorigenesis.
- Pathways
Various studies indicate that claudin 6 is involved in the Wnt signaling pathway which is key in embryonic development and cell differentiation. This pathway interacts with several other proteins including β-catenin to mediate gene expression and cellular functions. Additionally claudin 6 contributes to pathways influencing the epithelial-mesenchymal transition (EMT) a process necessary for organ development and cancer metastasis. Through these pathways claudin 6 interacts with diverse proteins to modulate cell behavior.
- Associated diseases and disorders
Researchers have linked claudin 6 to certain cancers particularly ovarian and breast cancer. Its aberrant expression may contribute to tumor progression and invasiveness. Claudin 6 can promote changes in the tumor microenvironment by interacting with other cell junction proteins possibly influencing cancer cell proliferation and spread. Furthermore its altered expression is noted in immune disorders suggesting potential roles in pathogenesis through interactions with immune cells or signaling molecules like cytokines within the immune pathways.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Recombinant human Claudin 6 protein - Active (His tag) (ab314532)
All lanes: mouse anti-6*His monoclonal antibody
All lanes: Western blot - Recombinant human Claudin 6 protein - Active (His tag) (ab314532)
Functional Studies - Recombinant human Claudin 6 protein - Active (His tag) (ab314532)
Measured by its binding ability in a functional ELISA. ab314532 at 10 μg/mL can bind an Anti-CLDN6/9 recombinant antibody, the EC50 is 1.501-2.035 ng/mL
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com