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AB160326

Recombinant Human Claudin 8 protein

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Recombinant Human Claudin 8 protein is a Human Full Length protein, in the 1 to 225 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

UNQ779/PRO1573, CLDN8, Claudin-8

1 Images
SDS-PAGE - Recombinant Human Claudin 8 protein (AB160326)
  • SDS-PAGE

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SDS-PAGE - Recombinant Human Claudin 8 protein (AB160326)

ab160326 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, ELISA

applications

Biologically active

No

Accession

P56748

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MATHALEIAGLFLGGVGMVGTVAVTVMPQWRVSAFIENNIVVFENFWEGLWMNCVRQANIRMQCKIYDSLLALSPDLQAARGLMCAASVMSFLAFMMAILGMKCTRCTGDNEKVKAHILLTAGIIFIITGMVVLIPVSWVANAIIRDFYNSIVNVAQKRELGEALYLGWTTALVLIVGGALFCCVFCCNEKSSSYRYSIPSHRTTQKSYHTGKKSPSVYSRSQYV","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":225,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P56748","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Claudin 8 also known as CLDN8 is a protein that functions as a critical component of tight junction strands which are integral to maintaining cell-to-cell adhesion. This protein belongs to the claudin family characterized by their role in forming the backbone of tight junctions. Claudin 8 has a molecular mass of approximately 23 kDa. It is expressed in several tissues including the kidney and gastrointestinal tract epithelium where it regulates selective permeability.
Biological function summary

Claudin 8 contributes to the creation and maintenance of the barrier function of epithelial layers. It integrates into tight junction complexes collaborating with other claudins and occludin to establish selective paracellular transport. This organization allows for the regulation of movement of ions and small solutes between cells essential in controlling the internal environment of organs.

Pathways

Research shows Claudin 8 plays a role in epithelial and endothelial cell permeability. It is involved in the calcium signaling pathway and the MAPK (Mitogen-Activated Protein Kinase) pathway both important for cell signaling and response to extracellular stimuli. Within these pathways Claudin 8 interacts with proteins such as ZO-1 (Zonula Occludens-1) to modulate tight junction integrity and function.

Defects or changes in Claudin 8 expression have associations with conditions like hypermagnesemia with seizures and mental retardation as well as certain types of cancer. In these contexts alterations in the protein's function can disrupt normal paracellular transport and contribute to disease pathology. Additionally Claudin 8 also links with occludin another component of tight junctions suggesting that aberrant interactions between these proteins might underpin certain disease processes.
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Specifications

Form

Liquid

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General info

Function

Can associate with other claudins to regulate tight junction structural and functional strand dynamics (By similarity). May coassemble with CLDN4 into tight junction strands containing anion-selective channels that convey paracellular chloride permeability in renal collecting ducts (By similarity) (PubMed : 36008380). Cannot form tight junction strands on its own (PubMed : 36008380).

Sequence similarities

Belongs to the claudin family.

Post-translational modifications

Ubiquitinated by the BCR(KLHL3) E3 ubiquitin ligase complex in the kidney, leading to its degradation.

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Product protocols

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Target data

Can associate with other claudins to regulate tight junction structural and functional strand dynamics (By similarity). May coassemble with CLDN4 into tight junction strands containing anion-selective channels that convey paracellular chloride permeability in renal collecting ducts (By similarity) (PubMed : 36008380). Cannot form tight junction strands on its own (PubMed : 36008380).
See full target information CLDN8
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