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AB314533

Recombinant human Claudin 9 protein - Active (His tag)

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Recombinant human Claudin 9 protein - Active (His tag) is a Human Full Length protein, in the 1 to 217 aa range, expressed in Mammalian, < 1 EU/µg endotoxin level, suitable for WB, FuncS.

View Alternative Names

Claudin-9, CLDN9

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Functional Studies - Recombinant human Claudin 9 protein - Active (His tag) (AB314533)
  • FuncS

Supplier Data

Functional Studies - Recombinant human Claudin 9 protein - Active (His tag) (AB314533)

Activity measured by its binding ability in a functional ELISA. Immobilized ab314533 at 10 µg/ml can bind an Anti-CLDN6/9 recombinant antibody, the EC50 is 24.93-35.47 ng/mL.

Western blot - Recombinant human Claudin 9 protein - Active (His tag) (AB314533)
  • WB

Supplier Data

Western blot - Recombinant human Claudin 9 protein - Active (His tag) (AB314533)

All lanes:

mouse anti-6*His monoclonal antibody

All lanes:

Western blot - Recombinant human Claudin 9 protein - Active (His tag) (ab314533)

false

Key facts

Endotoxin level

< 1 EU/µg

Expression system

Mammalian

Tags

His tag C-Terminus

Applications

FuncS, WB

applications

Biologically active

Yes

Biological activity

Measured by its binding ability in a functional ELISA. AB314533 at 10 μg/mL can bind an Anti-CLDN6/9 recombinant antibody, the EC50 is 24.93-35.47 ng/mL.

Accession

O95484

Animal free

Yes

Carrier free

No

Species

Human

Reconstitution

Reconstitute in water

Storage buffer

pH: 7.4 - 8 Constituents: 6% Trehalose, 0.14% PBS

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20degC/-80degC. Our default final concentration of glycerol is 50%.
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Sequence info

[{"sequence":"MASTGLELLGMTLAVLGWLGTLVSCALPLWKVTAFIGNSIVVAQVVWEGLWMSCVVQSTGQMQCKVYDSLLALPQDLQAARALCVIALLLALLGLLVAITGAQCTTCVEDEGAKARIVLTAGVILLLAGILVLIPVCWTAHAIIQDFYNPLVAEALKRELGASLYLGWAAAALLMLGGGLLCCTCPPPQVERPRGPRLGYSIPSRSGASGLDKRDYV","proteinLength":"Full Length","predictedMolecularWeight":"24.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":217,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Mammalian","accessionNumber":"O95484","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C|-80°C
Appropriate long-term storage conditions
-20°C|-80°C
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Claudin-9 also known as CLDN9 is a member of the claudin protein family. This protein plays an important role in forming tight junctions which are responsible for regulating paracellular permeability in epithelial and endothelial cell sheets. Claudin-9 has a predicted mass of approximately 23 kDa. It is primarily expressed in the inner ear specifically in the cochlea and has vital functions in maintaining the permeability barrier and the structural integrity of cell layers.
Biological function summary

Claudin-9 engages in maintaining the tight junction barrier function of epithelial cells. It operates within a complex interacting closely with other claudin family members to establish the selective paracellular barrier important for ion balance and cellular homeostasis. These interactions allow claudin-9 to contribute significantly to the physiology of tissues where it is expressed impacting hearing function through ion permeability regulation in the cochlear epithelium.

Pathways

Claudin-9 is an integral component of pathways involved in cell-to-cell adhesion and cell polarity maintenance. It participates prominently in the tight junction pathway which is tightly regulated for maintaining the fundamental structure of epithelial layers. In this context it works alongside related proteins such as occludin and certain zonula occludens proteins which fortify the overall structure and signaling pathways essential for epithelial cell function.

Mutations or dysregulation of claudin-9 have been implicated in particular hearing impairments. Sensorineural hearing loss a condition attributed to malfunction in the inner ear's sensory parts aligns with malfunctions in claudin-9 activity. This condition can also have connections to other proteins involved in cochlear biology such as K+ channel proteins indicating how disruptions in tight junction function can affect ion homeostasis and lead to auditory dysfunction.
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Specifications

Form

Lyophilized

Additional notes

Purification technique - Centrifugation.

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General info

Function

Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.. (Microbial infection) Acts as a receptor for hepatitis C virus (HCV) entry into hepatic cells.

Sequence similarities

Belongs to the claudin family.

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Product protocols

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Target data

Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.. (Microbial infection) Acts as a receptor for hepatitis C virus (HCV) entry into hepatic cells.
See full target information Claudin 9
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Complementary Products

Primary Antibodies

AB187116

Anti-Claudin 9 antibody [YD4E9] - BSA and Azide free

Flow Cytometry - Anti-Claudin 9 antibody [YD4E9] - BSA and Azide free (AB187116)

  • 5
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