Recombinant human Claudin 9 protein - Active (His tag) is a Human Full Length protein, in the 1 to 217 aa range, expressed in Mammalian, < 1 EU/µg endotoxin level and suitable for WB, FuncS.
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Key facts
- Endotoxin level
< 1 EU/µg
- Expression system
Mammalian
- Tags
His tag C-Terminus
- Applications
WB, FuncS
- Biologically active
Yes
Amino acid sequence
M A S T G L E L L G M T L A V L G W L G T L V S C A L P L W K V T A F I G N S I V V A Q V V W E G L W M S C V V Q S T G Q M Q C K V Y D S L L A L P Q D L Q A A R A L C V I A L L L A L L G L L V A I T G A Q C T T C V E D E G A K A R I V L T A G V I L L L A G I L V L I P V C W T A H A I I Q D F Y N P L V A E A L K R E L G A S L Y L G W A A A A L L M L G G G L L C C T C P P P Q V E R P R G P R L G Y S I P S R S G A S G L D K R D Y V
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.(Microbial infection) Acts as a receptor for hepatitis C virus (HCV) entry into hepatic cells.
Alternative names
Claudin-9, CLDN9
Recommended products
Recombinant human Claudin 9 protein - Active (His tag) is a Human Full Length protein, in the 1 to 217 aa range, expressed in Mammalian, < 1 EU/µg endotoxin level and suitable for WB, FuncS.
Alternative names
Claudin-9, CLDN9
Key facts
- Endotoxin level
< 1 EU/µg
- Expression system
Mammalian
- Tags
His tag C-Terminus
- Applications
WB, FuncS
- Biologically active
Yes
- Biological activity
Measured by its binding ability in a functional ELISA. AB314533 at 10 μg/mL can bind an Anti-CLDN6/9 recombinant antibody, the EC50 is 24.93-35.47 ng/mL.
- Accession
- O95484-1
- Animal free
Yes
- Species
Human
- Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%.
- Concentration
- Storage buffer
pH: 7.4 - 8
Constituents: 6% Trehalose, 0.14% PBS
Sequence info
Amino acid sequence
M A S T G L E L L G M T L A V L G W L G T L V S C A L P L W K V T A F I G N S I V V A Q V V W E G L W M S C V V Q S T G Q M Q C K V Y D S L L A L P Q D L Q A A R A L C V I A L L L A L L G L L V A I T G A Q C T T C V E D E G A K A R I V L T A G V I L L L A G I L V L I P V C W T A H A I I Q D F Y N P L V A E A L K R E L G A S L Y L G W A A A A L L M L G G G L L C C T C P P P Q V E R P R G P R L G Y S I P S R S G A S G L D K R D Y V
- Accession
- Protein length
Full Length
- Predicted molecular weight
24.2 kDa
- Amino acids
1 to 217
- Nature
Recombinant
- Tags
His tag C-Terminus
Specifications
- Form
Lyophilized
- Additional notes
Purification technique - Centrifugation.
General info
- Function
Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
- Sequence similarities
Belongs to the claudin family.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C, -80°C
- Storage information
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20degC/-80degC. Our default final concentration of glycerol is 50%.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Claudin-9 also known as CLDN9 is a member of the claudin protein family. This protein plays an important role in forming tight junctions which are responsible for regulating paracellular permeability in epithelial and endothelial cell sheets. Claudin-9 has a predicted mass of approximately 23 kDa. It is primarily expressed in the inner ear specifically in the cochlea and has vital functions in maintaining the permeability barrier and the structural integrity of cell layers.
- Biological function summary
Claudin-9 engages in maintaining the tight junction barrier function of epithelial cells. It operates within a complex interacting closely with other claudin family members to establish the selective paracellular barrier important for ion balance and cellular homeostasis. These interactions allow claudin-9 to contribute significantly to the physiology of tissues where it is expressed impacting hearing function through ion permeability regulation in the cochlear epithelium.
- Pathways
Claudin-9 is an integral component of pathways involved in cell-to-cell adhesion and cell polarity maintenance. It participates prominently in the tight junction pathway which is tightly regulated for maintaining the fundamental structure of epithelial layers. In this context it works alongside related proteins such as occludin and certain zonula occludens proteins which fortify the overall structure and signaling pathways essential for epithelial cell function.
- Associated diseases and disorders
Mutations or dysregulation of claudin-9 have been implicated in particular hearing impairments. Sensorineural hearing loss a condition attributed to malfunction in the inner ear's sensory parts aligns with malfunctions in claudin-9 activity. This condition can also have connections to other proteins involved in cochlear biology such as K+ channel proteins indicating how disruptions in tight junction function can affect ion homeostasis and lead to auditory dysfunction.
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2 product images
Western blot - Recombinant human Claudin 9 protein - Active (ab314533)
All lanes: mouse anti-6*His monoclonal antibody
All lanes: Western blot - Recombinant human Claudin 9 protein - Active (ab314533)
Functional Studies - Recombinant human Claudin 9 protein - Active (ab314533)
Activity measured by its binding ability in a functional ELISA. Immobilized ab314533 at 10 µg/ml can bind an Anti-CLDN6/9 recombinant antibody, the EC50 is 24.93-35.47 ng/mL.
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