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AB314616

Recombinant Human CLCN3 Protein (His Tag)

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Recombinant Human CLCN3 Protein (His Tag) is a Human Full Length protein, in the 1 to 818 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

H(+)/Cl(-) exchange transporter 3, Chloride channel protein 3, Chloride transporter ClC-3, ClC-3, CLCN3

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SDS-PAGE - Recombinant Human CLCN3 Protein (His Tag) (AB314616)
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Supplier Data

SDS-PAGE - Recombinant Human CLCN3 Protein (His Tag) (AB314616)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. It is speculated that the protein forms a multimer structure. Observed band size : Monomer：100kDa Trimer：300 kDa

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

6x His tag C-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Animal free

Yes

Carrier free

No

Species

Human

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%.

Storage buffer

pH: 7.4 - 8 Constituents: PBS, 6% Trehalose

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Complementary Products

Primary Antibodies

AB315881

Anti-CLC3 antibody [EPR26497-64]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunoprecipitation - Anti-CLC3 antibody [EPR26497-64] (AB315881)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"linker":null,"sequence":"MASMTGGQQMGRDPMESEQLFHRGYYRNSYNSITSASSDEELLDGAGVIMDFQTSEDDNLLDGDTAVGTHYTMTNGGSINSSTHLLDLLDEPIPGVGTYDDFHTIDWVREKCKDRERHRRINSKKKESAWEMTKSLYDAWSGWLVVTLTGLASGALAGLIDIAADWMTDLKEGICLSALWYNHEQCCWGSNETTFEERDKCPQWKTWAELIIGQAEGPGSYIMNYIMYIFWALSFAFLAVSLVKVFAPYACGSGIPEIKTILSGFIIRGYLGKWTLMIKTITLVLAVASGLSLGKEGPLVHVACCCGNIFSYLFPKYSTNEAKKREVLSAASAAGVSVAFGAPIGGVLFSLEEVSYYFPLKTLWRSFFAALVAAFVLRSINPFGNSRLVLFYVEYHTPWYLFELFPFILLGVFGGLWGAFFIRANIAWCRRRKSTKFGKYPVLEVIIVAAITAVIAFPNPYTRLNTSELIKELFTDCGPLESSSLCDYRNDMNASKIVDDIPDRPAGIGVYSAIWQLCLALIFKIIMTVFTFGIKVPSGLFIPSMAIGAIAGRIVGIAVEQLAYYHHDWFIFKEWCEVGADCITPGLYAMVGAAACLGGVTRMTVSLVVIVFELTGGLEYIVPLMAAVMTSKWVGDAFGREGIYEAHIRLNGYPFLDAKEEFTHTTLAADVMRPRRNDPPLAVLTQDNMTVDDIENMINETSYNGFPVIMSKESQRLVGFALRRDLTIAIESARKKQEGIVGSSRVCFAQHTPSLPAESPRPLKLRSILDMSPFTVTDHTPMEIVVDIFRKLGLRQCLVTHNGRLLGIITKKDILRHMAQTANQDPASIMFN","proteinLength":"Full Length","predictedMolecularWeight":"93.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":818,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P51790","tags":[{"tag":"6x His","terminus":"C-Terminus"}]}]
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Properties and storage information

Form
Lyophilized
Purification technique
Ion exchange chromatography
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Add glycerol to a final volume of 50% for extra stability and aliquot
Storage information
The product can be stored for up to 12 months
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General info

Function

Isoform 1. Strongly outwardly rectifying, electrogenic H(+)/Cl(-)exchanger which mediates the exchange of chloride ions against protons (By similarity). The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons (PubMed : 29845874). The presence of conserved gating glutamate residues is typical for family members that function as antiporters (PubMed : 29845874).. Isoform 2. Strongly outwardly rectifying, electrogenic H(+)/Cl(-)exchanger which mediates the exchange of chloride ions against protons.

Sequence similarities

Belongs to the chloride channel (TC 2.A.49) family. ClC-3/CLCN3 subfamily.

Post-translational modifications

N-glycosylated.

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Product protocols

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Target data

Isoform 1. Strongly outwardly rectifying, electrogenic H(+)/Cl(-)exchanger which mediates the exchange of chloride ions against protons (By similarity). The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons (PubMed : 29845874). The presence of conserved gating glutamate residues is typical for family members that function as antiporters (PubMed : 29845874).. Isoform 2. Strongly outwardly rectifying, electrogenic H(+)/Cl(-)exchanger which mediates the exchange of chloride ions against protons.
See full target information CLCN3
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