Recombinant human Cleaved Caspase-3 protein (Active) is a Human Full Length protein, in the 1 to 277 aa range, expressed in Escherichia coli, with >95% purity and suitable for FuncS.
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- Nature methods 21:342-3522024Mapping enzyme activity in living systems by real-time mid-infrared photothermal imaging of nitrile chameleons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongjian He,Jiaze Yin,Mingsheng Li,Chinmayee Vallabh Prabhu Dessai,Meihui Yi,Xinyan Teng,Meng Zhang,Yueming Li,Zhiyi Du,Bing Xu,Ji-Xin ChengPubMed 38191931
- Molecular therapy. Nucleic acids 33:548-5582023Cathepsin B-activatable cyclic antisense oligonucleotides for cell-specific target gene knockdown and .Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhongyu Wang,Xinli Fan,Guanqun Mu,Xiaoran Zhao,Qian Wang,Jing Wang,Xinjing TangPubMed 37588686
- International journal of molecular sciences 24:2023Pharmacological Targeting of Bcl-2 Induces Caspase 3-Mediated Cleavage of HDAC6 and Regulates the Autophagy Process in Colorectal Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDonglin Yang,Liujun He,Shuiqing Ma,Shiqiang Li,Yajun Zhang,Chunsheng Hu,Jiuhong Huang,Zhigang Xu,Dianyong Tang,Zhongzhu ChenPubMed 37047634
- Cell & bioscience 12:1222022AKT phosphorylation as a predictive biomarker for PI3K/mTOR dual inhibition-induced proteolytic cleavage of mTOR companion proteins in small cell lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMing-Chun Hung,Wan-Ping Wang,Ya-Hui ChiPubMed 35918763
- Molecular cell 82:785-802.e102022SPARCLE, a p53-induced lncRNA, controls apoptosis after genotoxic stress by promoting PARP-1 cleavage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKarla F Meza-Sosa,Rui Miao,Francisco Navarro,Zhibin Zhang,Ying Zhang,Jun Jacob Hu,Corrine Corrina R Hartford,Xiao Ling Li,Gustavo Pedraza-Alva,Leonor Pérez-Martínez,Ashish Lal,Hao Wu,Judy LiebermanPubMed 35104452
- Experimental cell research 370:103-1152018Pseudo-phosphorylation at AT8 epitopes regulates the tau truncation at aspartate 421.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLan Cao et. al.PubMed 29908160
- ACS chemical biology 13:761-7712018Toward a Microparticle-Based System for Pooled Assays of Small Molecules in Cellular Contexts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarrie E Yozwiak et. al.PubMed 29365249
- Biochimica et biophysica acta 1840:191-82013Implications of the O-GlcNAc modification in the regulation of nuclear apoptosis in T cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBruno Johnson,Marlyse Opimba,Jacques BernierPubMed 24035784
Key facts
- Purity
>95% SDS-PAGE
- Expression system
Escherichia coli
- Tags
Tag free
- Applications
FuncS
- Biologically active
Yes
Amino acid sequence
M E N T E N S V D S K S I K N L E P K I I H G S E S M D S G I S L D N S Y K M D Y P E M G L C I I I N N K N F H K S T G M T S R S G T D V D A A N L R E T F R N L K Y E V R N K N D L T R E E I V E L M R D V S K E D H S K R S S F V C V L L S H G E E G I I F G T N G P V D L K K I T N F F R G D R C R S L T G K P K L F I I Q A C R G T E L D C G I E T D S G V D D D M A C H K I P V E A D F L Y A Y S T A P G Y Y S W R N S K D G S W F I Q S L C A M L K Q Y A D K L E F M H I L T R V N R K V A T E F E S F S F D A T F H A K K Q I P C I V S M L T K E L Y F Y H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application FuncS | Reactivity Reacts | Dilution info - | Notes We recommend using 1 unit/assay for analyzing caspase activity. |
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Target data
Function
Involved in the activation cascade of caspases responsible for apoptosis execution (PubMed:7596430). At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond (PubMed:7774019). Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9 (PubMed:7596430). Involved in the cleavage of huntingtin (PubMed:8696339). Triggers cell adhesion in sympathetic neurons through RET cleavage (PubMed:21357690). Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress (PubMed:23152800).
Alternative names
Caspase-3, CASP-3, Apopain, Cysteine protease CPP32, Protein Yama, SREBP cleavage activity 1, CPP-32, SCA-1, CASP3, CPP32
Recommended products
Recombinant human Cleaved Caspase-3 protein (Active) is a Human Full Length protein, in the 1 to 277 aa range, expressed in Escherichia coli, with >95% purity and suitable for FuncS.
Key facts
- Purity
>95% SDS-PAGE
- Expression system
Escherichia coli
- Tags
Tag free
- Applications
FuncS
- Biologically active
Yes
- Biological activity
Specific activity: ≥ 15,000 units/mg
Unit definition: One unit of the recombinant caspase-3 is the enzyme activity that cleaves 1 nmol of the caspase substrate DEVD-pNA (pNA: pnitroanaline) per hour at 37oC in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
- Accession
- P42574-1
- Animal free
No
- Species
Human
- Reconstitution
Centrifuge the vial prior to opening. Reconstitute to 1 unit per µl in water.
- Concentration
- Storage buffer
Constituents: 5% Glycerol (glycerin, glycerine), PBS, 1.71% HEPES, 1.71% Sodium chloride, 0.16% EDTA, 0.12% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.1% CHAPS
Sequence info
Amino acid sequence
M E N T E N S V D S K S I K N L E P K I I H G S E S M D S G I S L D N S Y K M D Y P E M G L C I I I N N K N F H K S T G M T S R S G T D V D A A N L R E T F R N L K Y E V R N K N D L T R E E I V E L M R D V S K E D H S K R S S F V C V L L S H G E E G I I F G T N G P V D L K K I T N F F R G D R C R S L T G K P K L F I I Q A C R G T E L D C G I E T D S G V D D D M A C H K I P V E A D F L Y A Y S T A P G Y Y S W R N S K D G S W F I Q S L C A M L K Q Y A D K L E F M H I L T R V N R K V A T E F E S F S F D A T F H A K K Q I P C I V S M L T K E L Y F Y H
- Accession
- Protein length
Full Length
- Amino acids
1 to 277
- Nature
Recombinant
Specifications
- Form
Lyophilized
General info
- Function
Involved in the activation cascade of caspases responsible for apoptosis execution (PubMed:7596430). At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond (PubMed:7774019). Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9 (PubMed:7596430). Involved in the cleavage of huntingtin (PubMed:8696339). Triggers cell adhesion in sympathetic neurons through RET cleavage (PubMed:21357690). Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress (PubMed:23152800).
- Sequence similarities
Belongs to the peptidase C14A family.
- Post-translational modifications
Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-80°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
This product is manufactured by BioVision, an Abcam company and was previously called 1083 Caspase-3, human recombinant. 1083-100 is the same size as the 100 unit size of ab52101.
The recombinant active human Caspase 3 spontaneously undergoes autoprocessing to yield subunits characteristic of the native enzyme.
Cleaved Caspase 3 is useful in studying enzyme regulation, determining target substrates, screening caspase inhibitors, or as a positive control in caspase activity assays. The active Caspase 3 preferentially cleaves Caspase 3 substrates e.g. DEVD-AFC or DEVD-pNA.
MW: large (17 kD) and small (11 kD) subunits.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Cleaved Caspase-3 also known as active caspase 3 plays an important role in the execution phase of cell apoptosis. The caspase 3 molecule has a molecular weight of approximately 32 kDa. It undergoes cleavage into subunits that are important for its activation marking its conversion into the cleaved form. Cleaved caspase is widely expressed in various tissues with a notable presence in the cytoplasm of cells undergoing apoptotic processes. Cleavage of caspase 3 results in the formation of a functional enzyme that participates in the degradation of cellular components during apoptosis.
- Biological function summary
Caspase 3 acts as an executioner enzyme in the process of programmed cell death. Its cleavage from the inactive zymogen form into the active form triggers apoptotic pathways ensuring the removal of damaged or unnecessary cells. Cleaved caspase operates within a proteolytic cascade often in conjunction with other caspases such as caspase 9. Once activated caspase 3 targets and cleaves specific cellular substrates contributing to the characteristic morphological changes and DNA fragmentation associated with apoptosis.
- Pathways
Cleaved caspase 3 is significant within the intrinsic and extrinsic apoptotic pathways. It acts downstream of initiator caspases such as caspase 9 in the intrinsic pathway where its activation involves mitochondrial signals. In the extrinsic pathway it closely associates with caspase 8 mediating cell death signals from extracellular triggers. These pathways ensure that cleaved caspase 3 fulfills its role as a critical mediator of apoptosis linking upstream death signals to cellular dismantling during the apoptosis process.
- Associated diseases and disorders
Cleaved caspase 3 connects to conditions such as cancer and neurodegenerative diseases. In cancer dysregulation of caspase 3 activity may lead to evasion of apoptosis facilitating tumor progression. Therapeutic approaches aim to restore its apoptotic functions in cancer cells. In neurodegenerative diseases like Alzheimer's disease abnormal activation of cleaved caspase 3 contributes to neuronal loss. It interacts with proteins like amyloid precursor protein promoting the pathological changes associated with this disorder. Understanding these interactions provides insights into potential therapeutic avenues.
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1 product image
Western blot - Recombinant human Cleaved Caspase-3 protein (Active) (ab52101)
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with Biotin Anti-Caspase-3 antibody [E87] ab195905 overnight at 4°C. Antibody binding to the target was detected using a HRP conjugated streptavidin amplification step, and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.
All lanes: Western blot - Biotin Anti-Caspase-3 antibody [E87] (Biotin Anti-Caspase-3 antibody [E87] ab195905) at 1/10000 dilution
All lanes: Western blot - Recombinant human Cleaved Caspase-3 protein (Active) (ab52101) at 0.1 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 31 kDa
Observed band size: 15 kDa
Exposure time: 10s
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