Recombinant Human CLEC4A protein is a Human Full Length protein, in the 1 to 237 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Images
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
M T S E I T Y A E V R F K N E F K S S G I N T A S S A A S K E R T A P H K S N T G F P K L L C A S L L I F F L L L A I S F F I A F V I F F Q K Y S Q L L E K K T T K E L V H T T L E C V K K N M P V E E T A W S C C P K N W K S F S S N C Y F I S T E S A S W Q D S E K D C A R M E A H L L V I N T Q E E Q D F I F Q N L Q E E S A Y F V G L S D P E G Q R H W Q W V D Q T P Y N E S S T F W H P R E P S D P N E R C V V L N F R K S P K R W G W N D V N C L G P Q R S V C E M M K I H L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
C-type lectin receptor that binds carbohydrates mannose and fucose but also weakly interacts with N-acetylglucosamine (GlcNAc) in a Ca(2+)-dependent manner (PubMed:27015765). Involved in regulating immune reactivity (PubMed:10438934, PubMed:18258799). Once triggered by antigen, it is internalized by clathrin-dependent endocytosis and delivers its antigenic cargo into the antigen presentation pathway resulting in cross-priming of CD8(+) T cells. This cross-presentation and cross-priming are enhanced by TLR7 and TLR8 agonists with increased expansion of the CD8(+) T cells, high production of IFNG and TNF with reduced levels of IL4, IL5 and IL13 (PubMed:18258799, PubMed:20530286). In plasmacytoid dendritic cells, inhibits TLR9-mediated IFNA and TNF production (PubMed:18258799). May be involved via its ITIM motif (immunoreceptor tyrosine-based inhibitory motifs) in the inhibition of B-cell-receptor-mediated calcium mobilization and protein tyrosine phosphorylation (PubMed:10438934). (Microbial infection) Involved in the interaction between HIV-1 virus and dendritic cells. Enhances HIV-1 binding/entry and virus infection. Requires ITIM motif-associated signal transduction pathway involving phosphatases PTPN6 and PTPN11, SYK, Src kinases and MAP kinases.
Alternative names
CD367, CLECSF6, DCIR, LLIR, HDCGC13P, CLEC4A, C-type lectin domain family 4 member A, C-type lectin DDB27, C-type lectin superfamily member 6, Dendritic cell immunoreceptor, Lectin-like immunoreceptor
Recommended products
Recombinant Human CLEC4A protein is a Human Full Length protein, in the 1 to 237 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- Q9UMR7-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M T S E I T Y A E V R F K N E F K S S G I N T A S S A A S K E R T A P H K S N T G F P K L L C A S L L I F F L L L A I S F F I A F V I F F Q K Y S Q L L E K K T T K E L V H T T L E C V K K N M P V E E T A W S C C P K N W K S F S S N C Y F I S T E S A S W Q D S E K D C A R M E A H L L V I N T Q E E Q D F I F Q N L Q E E S A Y F V G L S D P E G Q R H W Q W V D Q T P Y N E S S T F W H P R E P S D P N E R C V V L N F R K S P K R W G W N D V N C L G P Q R S V C E M M K I H L
- Accession
- Q9UMR7
- Protein length
- Full Length
- Amino acids
- 1 to 237
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
C-type lectin receptor that binds carbohydrates mannose and fucose but also weakly interacts with N-acetylglucosamine (GlcNAc) in a Ca(2+)-dependent manner (PubMed:27015765). Involved in regulating immune reactivity (PubMed:10438934, PubMed:18258799). Once triggered by antigen, it is internalized by clathrin-dependent endocytosis and delivers its antigenic cargo into the antigen presentation pathway resulting in cross-priming of CD8(+) T cells. This cross-presentation and cross-priming are enhanced by TLR7 and TLR8 agonists with increased expansion of the CD8(+) T cells, high production of IFNG and TNF with reduced levels of IL4, IL5 and IL13 (PubMed:18258799, PubMed:20530286). In plasmacytoid dendritic cells, inhibits TLR9-mediated IFNA and TNF production (PubMed:18258799). May be involved via its ITIM motif (immunoreceptor tyrosine-based inhibitory motifs) in the inhibition of B-cell-receptor-mediated calcium mobilization and protein tyrosine phosphorylation (PubMed:10438934).
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
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1 product image
SDS-PAGE - Recombinant Human CLEC4A protein (ab162371)
ab162371 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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Product protocols
- Visit the general protocols
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