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AB95486

Recombinant Human CLIC1 protein (His tag N-Terminus)

5

(1 Review)

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(1 Publication)

Recombinant Human CLIC1 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 241 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

G6, NCC27, CLIC1, Chloride intracellular channel protein 1, Chloride channel ABP, Glutaredoxin-like oxidoreductase CLIC1, Glutathione-dependent dehydroascorbate reductase CLIC1, Nuclear chloride ion channel 27, Regulatory nuclear chloride ion channel protein, hRNCC

1 Images
SDS-PAGE - Recombinant Human CLIC1 protein (His tag N-Terminus) (AB95486)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human CLIC1 protein (His tag N-Terminus) (AB95486)

15% SDS-PAGE analysis of ab95486 (3ug)

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

O00299

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMAEEQPQVELFVKAGSDGAKIGNCPFSQRLFMVLWLKGVTFNVTTVDTKRRTETVQKLCPGGQLPFLLYGTEVHTDTNKIEEFLEAVLCPPRYPKLAALNPESNTAGLDIFAKFSAYIKNSNPALNDNLEKGLLKALKVLDNYLTSPLPEEVDETSAEDEGVSQRKFLDGNELTLADCNLLPKLHIVQVVCKKYRGFTIPEAFRGVHRYLSNAYAREEFASTCPDDEEIELAYEQVAKALK","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":241,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O00299","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

Additional notes

ab95486 was purified using conventional chromatography techniques

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General info

Function

In the soluble state, catalyzes glutaredoxin-like thiol disulfide exchange reactions with reduced glutathione as electron donor. Reduces selenite and dehydroascorbate and may act as an antioxidant during oxidative stress response (PubMed : 25581026, PubMed : 37759794). Can insert into membranes and form voltage-dependent multi-ion conductive channels. Membrane insertion seems to be redox-regulated and may occur only under oxidizing conditions. Involved in regulation of the cell cycle.

Sequence similarities

Belongs to the chloride channel CLIC family.

Post-translational modifications

Hydrogen peroxide treatment causes a conformation change, leading to dimerization and formation of an intramolecular disulfide bond between Cys-24 and Cys-59.

Subcellular localisation

Nucleus

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Product protocols

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Target data

In the soluble state, catalyzes glutaredoxin-like thiol disulfide exchange reactions with reduced glutathione as electron donor. Reduces selenite and dehydroascorbate and may act as an antioxidant during oxidative stress response (PubMed : 25581026, PubMed : 37759794). Can insert into membranes and form voltage-dependent multi-ion conductive channels. Membrane insertion seems to be redox-regulated and may occur only under oxidizing conditions. Involved in regulation of the cell cycle.
See full target information CLIC1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Science advances 10:eadp0696 PubMed39356770

2024

Identification of an ionic mechanism for ERα-mediated rapid excitation in neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Yu,Na Yin,Bing Feng,Peiyu Gao,Kaifan Yu,Hesong Liu,Hailan Liu,Yongxiang Li,Olivia Z Ginnard,Kristine M Conde,Mengjie Wang,Xing Fang,Longlong Tu,Jonathan C Bean,Qingzhuo Liu,Yue Deng,Yuxue Yang,Junying Han,Sanika V Jossy,Megan L Burt,Huey Zhong Wong,Yongjie Yang,Benjamin R Arenkiel,Yang He,Shaodong Guo,Pierre Gourdy,Jean-Francois Arnal,Francoise Lenfant,Zhao Wang,Chunmei Wang,Yanlin He,Yong Xu
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Complementary Products

Primary Antibodies

AB229917

Anti-CLIC1 antibody [EPR22907-50]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-CLIC1 antibody [EPR22907-50] (AB229917)

  • 5
  • 1
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

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  • 15
Proteins & Peptides

AB85273

Recombinant Human 14-3-3 alpha + beta protein

SDS-PAGE - Recombinant Human 14-3-3 alpha + beta protein (AB85273)

  • 0
  • 0
Proteins & Peptides

AB87361

Recombinant Human 14-3-3 zeta protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human 14-3-3 zeta protein (AB87361)

  • 0
  • 0

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