JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB85759

Recombinant human CLK3 protein

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant human CLK3 protein is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, WB, FuncS.

View Alternative Names

Dual specificity protein kinase CLK3, CDC-like kinase 3, CLK3

4 Images
Functional Studies - Recombinant human CLK3 protein (AB85759)
  • FuncS

Unknown

Functional Studies - Recombinant human CLK3 protein (AB85759)

The Specific activity of ab85759 was determined to be 75 nmol/min/mg.

Functional Studies - Recombinant human CLK3 protein (AB85759)
  • FuncS

Unknown

Functional Studies - Recombinant human CLK3 protein (AB85759)

The specific activity of CLK3 (ab85759) was determined to be 28.9 nmol/min/mg as per activity assay protocol and was equivalent to 80 nmol/min/mg as per radiometric assay

SDS-PAGE - Recombinant human CLK3 protein (AB85759)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human CLK3 protein (AB85759)

SDS-PAGE showing ab85759 at approximately 86kDa.

SDS-PAGE - Recombinant human CLK3 protein (AB85759)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human CLK3 protein (AB85759)

SDS PAGE analysis of ab85759

Key facts

Purity

>90% Densitometry

Expression system

Baculovirus infected Sf9 cells

Tags

Tag free

Applications

FuncS, SDS-PAGE, WB

applications

Biologically active

Yes

Biological activity

The Specific activity of ab85759 was determined to be 75 nmol/min/mg.

Accession

P49761

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0038% EGTA, 0.00292% EDTA, 0.00174% PMSF

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
style
left-column

Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P49761","tags":[]}]
style
left-column

Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
style
left-column
style
left-column

Specifications

Form

Liquid

Additional notes

Affinity purified.

style
left-column

General info

Function

Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex. May be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing and can cause redistribution of SR proteins from speckles to a diffuse nucleoplasmic distribution. Phosphorylates SRSF1 and SRSF3. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily.

Post-translational modifications

Autophosphorylates on all three types of residues.

Subcellular localisation

Nucleus

style
left-column

Product protocols

style
left-column

Target data

Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex. May be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing and can cause redistribution of SR proteins from speckles to a diffuse nucleoplasmic distribution. Phosphorylates SRSF1 and SRSF3. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.
See full target information CLK3
style
left-column
style
left-column
style
right-column

Complementary Products

Proteins & Peptides

AB101548

Recombinant human ULK3 protein

Functional Studies - Recombinant human ULK3 protein (AB101548)

  • 0
  • 0
Proteins & Peptides

AB84801

Recombinant human STK23 protein

Functional Studies - Recombinant human STK23 protein (AB84801)

  • 0
  • 0
Proteins & Peptides

AB85293

Recombinant human SRPK2 protein

Functional Studies - Recombinant human SRPK2 protein (AB85293)

  • 0
  • 0
Proteins & Peptides

AB271752

Recombinant human NAK/TBK1 protein (Active)

Functional Studies - Recombinant human NAK/TBK1 protein (Active) (AB271752)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com