AB169554

Recombinant Human CLLD8/SETDB2 protein

Name: Recombinant Human CLLD8/SETDB2 protein (ab169554) | Abcam
Brand: Abcam Limited
SKU: AB169554
Price: 360 USD
Availability: InStock

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(1 Publication)

Recombinant Human CLLD8/SETDB2 protein is a Human Full Length protein, in the 1 to 719 aa range, expressed in Baculovirus infected Sf9 cells, with >80%, suitable for SDS-PAGE.

View Alternative Names

C13orf4, CLLD8, KMT1F, SETDB2, Histone-lysine N-methyltransferase SETDB2, Chronic lymphocytic leukemia deletion region gene 8 protein, Lysine N-methyltransferase 1F, SET domain bifurcated 2

2 Images

Lab

Western blot - Recombinant Human CLLD8/SETDB2 protein (AB169554)

ab5517 recognises a band corresponding to the tagged CLLD8/SETB2 at approximately 120 kDa. Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

All lanes:

Western blot - Anti-CLLD8/SETDB2 antibody (<a href='/en-us/products/primary-antibodies/clld8-setdb2-antibody-ab5517'>ab5517</a>) at 1 µg/mL

All lanes:

Western blot - Recombinant Human CLLD8/SETDB2 protein (ab169554) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 82 kDa

Observed band size: 120 kDa

true

Exposure time: 10s

SDS-PAGE - Recombinant Human CLLD8/SETDB2 protein (AB169554)

SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human CLLD8/SETDB2 protein (AB169554)

SDS-PAGE analysis of ab169554.

Key facts

Purity

>80% Densitometry

Expression system

Baculovirus infected Sf9 cells

Applications

SDS-PAGE

applications

Biologically active

Accession

Q96T68

Animal free

Carrier free

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Sequence info

[{"sequence":"MGEKNGDAKTFWMELEDDGKVDFIFEQVQNVLQSLKQKIKDGSATNKEYIQAMILVNEATIINSSTSIKDPMPVTQKEQENKSNAFPSTSCENSFPEDCTFLTTGNKEILSLEDKVVDFREKDSSSNLSYQSHDCSGACLMKMPLNLKGENPLQLPIKCHFQRRHAKTNSHSSALHVSYKTPCGRSLRNVEEVFRYLLETECNFLFTDNFSFNTYVQLARNYPKQKEVVSDVDISNGVESVPISFCNEIDSRKLPQFKYRKTVWPRAYNLTNFSSMFTDSCDCSEGCIDITKCACLQLTARNAKTSPLSSDKITTGYKYKRLQRQIPTGIYECSLLCKCNRQLCQNRVVQHGPQVRLQVFKTEQKGWGVRCLDDIDRGTFVCIYSGRLLSRANTEKSYGIDENGRDENTMKNIFSKKRKLEVACSDCEVEVLPLGLETHPRTAKTEKCPPKFSNNPKELTMETKYDNISRIQYHSVIRDPESKTAIFQHNGKKMEFVSSESVTPEDNDGFKPPREHLNSKTKGAQKDSSSNHVDEFEDNLLIESDVIDITKYREETPPRSRCNQATTLDNQNIKKAIEVQIQKPQEGRSTACQRQQVFCDEELLSETKNTSSDSLTKFNKGNVFLLDATKEGNVGRFLNHSCCPNLLVQNVFVETHNRNFPLVAFFTNRYVKARTELTWDYGYEAGTVPEKEIFCQCGVNKCRKKIL","proteinLength":"Full Length","predictedMolecularWeight":"120 kDa","actualMolecularWeight":null,"aminoAcidEnd":719,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q96T68","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]

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Properties and storage information

Shipped at conditions

Dry Ice

Appropriate short-term storage conditions

-80°C

Appropriate long-term storage conditions

-80°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

False

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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Histone methyltransferase involved in left-right axis specification in early development and mitosis. Specifically trimethylates 'Lys-9' of histone H3 (H3K9me3). H3K9me3 is a specific tag for epigenetic transcriptional repression that recruits HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Contributes to H3K9me3 in both the interspersed repetitive elements and centromere-associated repeats. Plays a role in chromosome condensation and segregation during mitosis.

Sequence similarities

Belongs to the class V-like SAM-binding methyltransferase superfamily.

Subcellular localisation

Nucleus

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information SETDB2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Technology in cancer research & treatment 18:1533033819871300 PubMed31564215

2019

MiR-122 Promotes the Development of Colon Cancer by Targeting .

Applications

Unspecified application

Species

Unspecified reactive species

Hong Li,Xinhua Zhang,Zhao Jin,Tao Yin,Chuanyi Duan,Junwei Sun,Rui Xiong,Zilin Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Recombinant Human CLLD8/SETDB2 protein

Western blot - Recombinant Human CLLD8/SETDB2 protein (AB169554)

SDS-PAGE - Recombinant Human CLLD8/SETDB2 protein (AB169554)

Key facts

Purity

Expression system

Tags

Applications

Biologically active

Accession

Animal free

Carrier free

Species

Storage buffer

Reactivity data

Sequence info

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Specifications

Form

Additional notes

General info

Function

Sequence similarities

Subcellular localisation

Product protocols

Target data

Publications (1)

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