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AB137159

Recombinant Human CLNS1A/CLCI protein

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Recombinant Human CLNS1A/CLCI protein is a Human Full Length protein, in the 1 to 237 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

CLCI, ICLN, CLNS1A, Methylosome subunit pICln, Chloride conductance regulatory protein ICln, Chloride ion current inducer protein, Reticulocyte pICln, I(Cln), ClCI

1 Images
SDS-PAGE - Recombinant Human CLNS1A/CLCI protein (AB137159)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human CLNS1A/CLCI protein (AB137159)

15% SDS-PAGE analysis of ab137159 (3μg)

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

P54105

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.003% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as CLNS1A
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSHMSFLKSFPPPGPAEGLLRQQPDTEAVLNGKGLGTGTLYIAESRLSWLDGSGLGFSLEYPTISLHALSRDRSDCLGEHLYVMVNAKFEEESKEPVADEEEEDSDDDVEPITEFRFVPSDKSALEAMFTAMCECQALHPDPEDEDSDDYDGEEYDVEAHEQGQGDIPTFYTYEEGLSHLTAEGQATLERLEGMLSQSVSSQYNMAGVRTEDSIRDYEDGMEVDTTPTVAGQFEDADVDH","proteinLength":"Full Length","predictedMolecularWeight":"28.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":237,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P54105","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

Additional notes

ab137159 was purified using conventional chromatography.

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General info

Function

Involved in both the assembly of spliceosomal snRNPs and the methylation of Sm proteins (PubMed : 10330151, PubMed : 11713266, PubMed : 18984161, PubMed : 21081503). Chaperone that regulates the assembly of spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs (PubMed : 10330151, PubMed : 18984161). Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core) (PubMed : 10330151). In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP (PubMed : 10330151, PubMed : 18984161). Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus (PubMed : 10330151, PubMed : 18984161).

Sequence similarities

Belongs to the pICln (TC 1.A.47) family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Involved in both the assembly of spliceosomal snRNPs and the methylation of Sm proteins (PubMed : 10330151, PubMed : 11713266, PubMed : 18984161, PubMed : 21081503). Chaperone that regulates the assembly of spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs (PubMed : 10330151, PubMed : 18984161). Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core) (PubMed : 10330151). In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP (PubMed : 10330151, PubMed : 18984161). Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus (PubMed : 10330151, PubMed : 18984161).
See full target information CLNS1A
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Complementary Products

Primary Antibodies

AB192907

Anti-CLNS1A/CLCI antibody - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLNS1A/CLCI antibody - C-terminal (AB192907)

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