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AB283456

Recombinant human CNTF protein (Active)

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Recombinant human CNTF protein (Active) is a Human Full Length protein, in the 2 to 200 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, Mass Spec, HPLC, Cell Culture.

View Alternative Names

Ciliary neurotrophic factor, CNTF

4 Images
Functional Studies - Recombinant human CNTF protein (Active) (AB283456)
  • FuncS

Supplier Data

Functional Studies - Recombinant human CNTF protein (Active) (AB283456)

Fully biologically active determined by dose dependent proliferation of TF-1 cells. ED50 is ≤4.914 μg/ml, corresponding to a specific activity of 2.04 x 105 units/mg.

Mass Spectrometry - Recombinant human CNTF protein (Active) (AB283456)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant human CNTF protein (Active) (AB283456)

Mass determination by ESI-TOF.

Predicted MW is 22856.99 Da (+/- 10 Da by ESI-TOF). Observed MW is 22858.40 Da

SDS-PAGE - Recombinant human CNTF protein (Active) (AB283456)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human CNTF protein (Active) (AB283456)

SDS-page analysis of ab283456

HPLC - Recombinant human CNTF protein (Active) (AB283456)
  • HPLC

Supplier Data

HPLC - Recombinant human CNTF protein (Active) (AB283456)

HPLC analysis of ab283456

Key facts

Purity

>95% HPLC

Endotoxin level

<0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

SDS-PAGE, Mass Spec, Cell Culture, FuncS, HPLC

applications

Biologically active

Yes

Biological activity

Fully biologically active determined by dose dependent proliferation of TF-1 cells. ED50 is ≤4.914 μg/ml, corresponding to a specific activity of 2.04 x 105 units/mg.

Accession

P26441

Animal free

Yes

Carrier free

No

Species

Human

Reconstitution

Reconstitute in PBS

Storage buffer

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Cell Culture": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"AFTEHSPLTPHRRDLCSRSIWLARKIRSDLTALTESYVKHQGLNKNINLDSADGMPVASTDQWSELTEAERLQENLQAYRTFHVLLARLLEDQQVHFTPTEGDFHQAIHTLLLQVAAFAYQIEELMILLEYKIPRNEADGMPINVGDGGLFEKKLWGLKVLQELSQWTVRSIHDLRFISSHQTGIPARGSHYIANNKKM","proteinLength":"Full Length","predictedMolecularWeight":"22.86 kDa","actualMolecularWeight":"22.86 kDa","aminoAcidEnd":200,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P26441","tags":[]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
True
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Specifications

Form

Lyophilized

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General info

Function

CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.

Sequence similarities

Belongs to the CNTF family.

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Product protocols

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Target data

CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.
See full target information CNTF
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Product promise

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