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Recombinant Human COASY protein is a Human Fragment protein, in the 296 to 564 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.

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Key facts

Purity
>90% SDS-PAGE
Expression system
Escherichia coli
Tags
T7 tag N-Terminus
Applications
SDS-PAGE
Biologically active
No

Amino acid sequence

M A S M T G G Q Q M G R G E F M A I N R F R L E N D L E E L A L Y Q I Q L L K D L R H T E N E E D K V S S S S F R Q R M L G N L L R P P Y E R P E L P T C L Y V I G L T G I S G S G K S S I A Q R L K G L G A F V I D S D H L G H R A Y A P G G P A Y Q P V V E A F G T D I L H K D G I I N R K V L G S R V F G N K K Q L K I L T D I M W P I I A K L A R E E M D R A V A E G K R V C V I D A A V L L E A G W Q N L V H E V W T A V I P E T E A V R R I V E R D G L S E A A A Q S R L Q S Q M S G Q Q L V E Q S H V V L S T L W E P H I T Q R Q V E K A W A L L Q K R I P K T H Q A L D

Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Target data

Function

Bifunctional enzyme that catalyzes the fourth and fifth sequential steps of CoA biosynthetic pathway. The fourth reaction is catalyzed by the phosphopantetheine adenylyltransferase, coded by the coaD domain; the fifth reaction is catalyzed by the dephospho-CoA kinase, coded by the coaE domain. May act as a point of CoA biosynthesis regulation.

Alternative names

Recommended products

Recombinant Human COASY protein is a Human Fragment protein, in the 296 to 564 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.

Key facts

Purity
>90% SDS-PAGE
Expression system
Escherichia coli
Applications
SDS-PAGE
Accession
Q13057-1
Animal free
No
Species
Human
Concentration
Loading...
Storage buffer

pH: 8
Constituents: 0.32% Tris HCl

Sequence info

Amino acid sequence

M A S M T G G Q Q M G R G E F M A I N R F R L E N D L E E L A L Y Q I Q L L K D L R H T E N E E D K V S S S S F R Q R M L G N L L R P P Y E R P E L P T C L Y V I G L T G I S G S G K S S I A Q R L K G L G A F V I D S D H L G H R A Y A P G G P A Y Q P V V E A F G T D I L H K D G I I N R K V L G S R V F G N K K Q L K I L T D I M W P I I A K L A R E E M D R A V A E G K R V C V I D A A V L L E A G W Q N L V H E V W T A V I P E T E A V R R I V E R D G L S E A A A Q S R L Q S Q M S G Q Q L V E Q S H V V L S T L W E P H I T Q R Q V E K A W A L L Q K R I P K T H Q A L D
Accession
Q13057
Protein length
Fragment
Predicted molecular weight
31.8 kDa
Amino acids
296 to 564
Nature
Recombinant
Tags
T7 tag N-Terminus

Specifications

Form
Liquid
Additional notes

This protein was expressed in E. coli as inclusion bodies, refolded using unique “temperature shift inclusion body refolding” technology and chromatographically purified.

General info

Function

Bifunctional enzyme that catalyzes the fourth and fifth sequential steps of CoA biosynthetic pathway. The fourth reaction is catalyzed by the phosphopantetheine adenylyltransferase, coded by the coaD domain; the fifth reaction is catalyzed by the dephospho-CoA kinase, coded by the coaE domain. May act as a point of CoA biosynthesis regulation.

Sequence similarities

In the central section; belongs to the eukaryotic CoaD family.

Subcellular localisation
Mitochondrion matrix

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-80°C

Product promise

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