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AB111635

Recombinant Human Cofilin 2 protein (His tag N-Terminus)

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(1 Publication)

Recombinant Human Cofilin 2 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 166 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

Cofilin-2, CFL2

1 Images
SDS-PAGE - Recombinant Human Cofilin 2 protein (His tag N-Terminus) (AB111635)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Cofilin 2 protein (His tag N-Terminus) (AB111635)

15% SDS-PAGE showing ab111635 at approximately 20.9kDa (3μg).

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Q9Y281

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.32% Tris HCl, 0.29% Sodium chloride, 0.04% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMASGVTVNDEVIKVFNDMKVRKSSTQEEIKKRKKAVLFCLSDDKRQIIVEEAKQILVGDIGDTVEDPYTSFVKLLPLNDCRYALYDATYETKESKKEDLVFIFWAPESAPLKSKMIYASSKDAIKKKFTGIKHEWQVNGLDDIKDRSTLGEKLGGNVVVSLEGKPL","proteinLength":"Full Length","predictedMolecularWeight":"20.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":166,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9Y281","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cofilin 2 also referred to as CFL2 is an actin-binding protein that plays a significant role in actin dynamics by disassembling actin filaments. It has a molecular mass of approximately 19 kDa. This protein is expressed predominantly in muscle tissues including skeletal and cardiac muscle. Cofilin 2 is integral in the maintenance and reorganization of the actin cytoskeleton facilitating cellular movement and shape changes.
Biological function summary

Cofilin 2 is involved in processes including muscle contraction and cytoskeletal organization. It participates in the depolymerization of actin filaments thereby accelerating filament turnover and recycling. It does not function in isolation but interacts with several proteins in the cytoskeleton forming a complex interplay that modulates actin filament dynamics. Its activity is regulated by phosphorylation with specific kinases and phosphatases modulating its function based on cellular signals.

Pathways

Cofilin 2 is an important element of the actin reorganization pathway and is involved in the Rho family GTPase signaling pathway. It works closely with proteins like LIM kinase which phosphorylates cofilin and inhibits its activity and slingshot phosphatase which dephosphorylates and activates it. This regulation ensures proper actin filament dynamics which is essential for processes like cell division migration and muscle function.

Cofilin 2 mutations have been associated with nemaline myopathy a muscle disorder characterized by muscle weakness. This disorder often involves abnormalities in the neuromuscular junction where cofilin 2 interacts with actin and related proteins like tropomyosin affecting muscle contraction. Additionally altered cofilin 2 expression links to cardiac complications due to its role in cardiac muscle maintenance and actin filament dynamics.
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Specifications

Form

Liquid

Additional notes

ab111635 was purified using conventional chromatography techniques.

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General info

Function

Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. Its F-actin depolymerization activity is regulated by association with CSPR3 (PubMed : 19752190). It has the ability to bind G- and F-actin in a 1 : 1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods. Required for muscle maintenance. May play a role during the exchange of alpha-actin forms during the early postnatal remodeling of the sarcomere (By similarity).

Sequence similarities

Belongs to the actin-binding proteins ADF family.

Post-translational modifications

The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal.

Subcellular localisation

Nucleus matrix

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Product protocols

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Target data

Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. Its F-actin depolymerization activity is regulated by association with CSPR3 (PubMed : 19752190). It has the ability to bind G- and F-actin in a 1 : 1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods. Required for muscle maintenance. May play a role during the exchange of alpha-actin forms during the early postnatal remodeling of the sarcomere (By similarity).
See full target information CFL2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Research (Washington, D.C.) 7:0331 PubMed38550779

2024

DNA-PKcs Phosphorylates Cofilin2 to Induce Endothelial Dysfunction and Microcirculatory Disorder in Endotoxemic Cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Yingzhen Du,Pingjun Zhu,Yukun Li,Jiachi Yu,Tian Xia,Xing Chang,Hang Zhu,Ruibing Li,Qingyong He
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Complementary Products

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Primary Antibodies

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Proteins & Peptides

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Primary Antibodies

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Product promise

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