Recombinant Human Collagen I protein is a Human Fragment protein, in the 1021 to 1108 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
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- Cells, tissues, organs 209:101-1092020Decellularized Porcine Epiphyseal Plate-Derived Extracellular Matrix Powder: Synthesis and Characterization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesThai Thanh Hoang Thi,Diem Huong Tran Nguyen,Dinh Tien Dung Nguyen,Dai Hai Nguyen,Minh-Dung TruongPubMed 32541153
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
E G S P G R D G S P G A K G D R G E T G P A G P P G A P G A P G A P G P V G P A G K S G D R G E T G P A G P A G P V G P V G A R G P A G P Q G P R G D K G E T G E Q G D R G I K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Type I collagen is a member of group I collagen (fibrillar forming collagen).
Alternative names
Collagen alpha-1(I) chain, Alpha-1 type I collagen, COL1A1
Recommended products
Recombinant Human Collagen I protein is a Human Fragment protein, in the 1021 to 1108 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- P02452-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- E G S P G R D G S P G A K G D R G E T G P A G P P G A P G A P G A P G P V G P A G K S G D R G E T G P A G P A G P V G P V G A R G P A G P Q G P R G D K G E T G E Q G D R G I K
- Accession
- P02452
- Protein length
- Fragment
- Predicted molecular weight
- 35.42 kDa
- Amino acids
- 1021 to 1108
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Glutathione Sepharose 4 Fast Flow
General info
- Function
Type I collagen is a member of group I collagen (fibrillar forming collagen).
- Sequence similarities
Belongs to the fibrillar collagen family.
- Post-translational modifications
Contains mostly 4-hydroxyproline. Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Collagen type I also called collagen I is a structural protein expressed mainly in connective tissues such as skin tendon bone and ligaments. It serves as an important component in providing mechanical strength and integrity to these tissues. Collagen I is a fibrillar collagen known for its triple-helix structure composed of two alpha-1 chains and one alpha-2 chain and has a molecular mass of approximately 300 kDa. Researchers often employ collagen western blot and collagen ELISA techniques for its detection. Collagen suppliers offer various collagen antibodies used in these assays to study its distribution and function.
- Biological function summary
Collagen type I plays a central role in maintaining the extracellular matrix and supporting cellular environments. It interacts with other matrix proteins and cells forming complexes that help in tissue development and repair. Type I collagen is especially important in bone matrix working alongside minerals like hydroxyapatite to provide rigidity and support. Anti-collagen antibodies aid in studying its biological functions and interactions which are critical to understanding tissue dynamics.
- Pathways
Collagen type I interacts with multiple signaling cascades involved in tissue remodeling and repair. It is a significant player in the TGF-β pathway which regulates fibrosis and wound healing processes. In these pathways proteins such as fibronectin and integrins work in concert with collagen type I to orchestrate cellular responses to damage. Researchers often examine its role in these pathways to uncover therapeutic possibilities for disease interventions.
- Associated diseases and disorders
Collagen type I has strong connections to conditions like osteogenesis imperfecta and fibrosis. Mutations or irregularities in collagen I production can lead to osteogenesis imperfecta a genetic disorder characterized by brittle bones. In fibrosis excessive collagen deposition disrupts normal tissue architecture contributing to organ dysfunction. In both conditions type I collagen interacts with other proteins like matrix metalloproteinases which modulate its breakdown and remodeling highlighting its importance in disease pathology.
Product promise
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1 product image
SDS-PAGE - Recombinant Human Collagen I protein (ab158152)
ab158152 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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