Recombinant Human Collagen III protein (Tagged) is a Human Fragment protein, in the 1059 to 1466 aa range, expressed in Escherichia coli, with >97% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >97% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- GST tag N-Terminus His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
G K S G D R G E S G P A G P A G A P G P A G S R G A P G P Q G P R G D K G E T G E R G A A G I K G H R G F P G N P G A P G S P G P A G Q Q G A I G S P G P A G P R G P V G P S G P P G K D G T S G H P G P I G P P G P R G N R G E R G S E G S P G H P G Q P G P P G P P G A P G P C C G G V G A A A I A G I G G E K A G G F A P Y Y G D E P M D F K I N T D E I M T S L K S V N G Q I E S L I S P D G S R K N P A R N C R D L K F C H P E L K S G E Y W V D P N Q G C K L D A I K V F C N M E T G E T C I S A N P L N V P R K H W W T D S S A E K K H V W F G E S M D G G F Q F S Y G N P E L P E D V L D V H L A F L R L L S S R A S Q N I T Y H C K N S I A Y M D Q A S G N V K K A L K L M G S N E G E F K A E G N S K F T Y T V L E D G C T K H T G E W S K T V F E Y R T R K A V R L P I V D I A P Y D I G G P D Q E F G V D V G P V C F L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12.
Alternative names
Collagen alpha-1(III) chain, COL3A1
Recommended products
Recombinant Human Collagen III protein (Tagged) is a Human Fragment protein, in the 1059 to 1466 aa range, expressed in Escherichia coli, with >97% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >97% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- GST tag N-Terminus His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P02461-1
- Animal free
- No
- Species
- Human
- Reconstitution
- Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
- Concentration
- Storage buffer
pH: 7.4
Constituents: 82.8% Trehalose, 16.38% PBS, 0.17% Sodium-N-Lauroylsarcosinate
Sequence info
Amino acid sequence
- G K S G D R G E S G P A G P A G A P G P A G S R G A P G P Q G P R G D K G E T G E R G A A G I K G H R G F P G N P G A P G S P G P A G Q Q G A I G S P G P A G P R G P V G P S G P P G K D G T S G H P G P I G P P G P R G N R G E R G S E G S P G H P G Q P G P P G P P G A P G P C C G G V G A A A I A G I G G E K A G G F A P Y Y G D E P M D F K I N T D E I M T S L K S V N G Q I E S L I S P D G S R K N P A R N C R D L K F C H P E L K S G E Y W V D P N Q G C K L D A I K V F C N M E T G E T C I S A N P L N V P R K H W W T D S S A E K K H V W F G E S M D G G F Q F S Y G N P E L P E D V L D V H L A F L R L L S S R A S Q N I T Y H C K N S I A Y M D Q A S G N V K K A L K L M G S N E G E F K A E G N S K F T Y T V L E D G C T K H T G E W S K T V F E Y R T R K A V R L P I V D I A P Y D I G G P D Q E F G V D V G P V C F L
- Accession
- P02461
- Protein length
- Fragment
- Predicted molecular weight
- 72.1 kDa
- Amino acids
- 1059 to 1466
- Nature
- Recombinant
- Tags
- GST tag N-Terminus, His tag N-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12.
- Sequence similarities
Belongs to the fibrillar collagen family.
- Post-translational modifications
Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Collagen type III also known as COL3 and collagen III is a fibrillar protein with an approximate molecular mass of 138 kDa. This protein is a significant component of the extracellular matrix and is mainly expressed in connective tissues like skin lungs and vascular walls. You can also find it in developing tissues and fibrotic tissues reflecting its role during tissue growth and repair processes.
- Biological function summary
Collagen type III contributes to maintaining the structural integrity of tissues. It forms a complex with collagen type I providing a scaffold that ensures mechanical strength and stability to tissues. This structural pairing of collagen type I and III highlights their cooperative function in supporting the extracellular matrix and regulating cell behavior in tissue repair and wound healing processes.
- Pathways
Collagen type III is integral to the processes of tissue repair and fibrosis pathways. It interacts closely with proteins such as fibronectin and integrins contributing to the cellular adhesion and signaling pathways important for fibroblast function and migration. These interactions reflect its essential role in matrix assembly and stability influencing how cells respond to injury and repair damaged tissues.
- Associated diseases and disorders
Collagen type III mutations or dysregulation associate with vascular Ehlers-Danlos syndrome and keloids. In vascular Ehlers-Danlos syndrome defective collagen III compromises vessel wall integrity resulting in vascular fragility and rupture. In the context of keloids excessive collagen III accumulation in the skin illustrates its role in pathological fibrosis. Collagen type I is another protein that plays an important role in these conditions contributing along with collagen type III to the altered structural and functional properties observed in these disorders.
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1 product image
SDS-PAGE - Recombinant Human Collagen III protein (Tagged) (ab275791)
SDS-PAGE analysis of ab275791.
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