Recombinant Human COMP/Cartilage oligomeric matrix protein is a Human Full Length protein, in the 21 to 757 aa range, expressed in HEK 293, with >90% purity and suitable for SDS-PAGE, WB.
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Publications
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- The Journal of cell biology 213:305-142016Secretory cargo sorting by Ca2+-dependent Cab45 oligomerization at the trans-Golgi network.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlvaro H Crevenna et. al.PubMed 27138253
- American journal of human genetics 92:366-742013ADAMTS7 cleavage and vascular smooth muscle cell migration is affected by a coronary-artery-disease-associated variant.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiangyuan Pu et. al.PubMed 23415669
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- HEK 293 cells
- Tags
- DDDDK tag N-Terminus
- Applications
- SDS-PAGE, WB
- Biologically active
- No
Amino acid sequence
H V D Y K D D D D K P A G Q G Q S P L G S D L G P Q M L R E L Q E T N A A L Q D V R E L L R Q Q V R E I T F L K N T V M E C D A C G M Q Q S V R T G L P S V R P L L H C A P G F C F P G V A C I Q T E S G A R C G P C P A G F T G N G S H C T D V N E C N A H P C F P R V R C I N T S P G F R C E A C P P G Y S G P T H Q G V G L A F A K A N K Q V C T D I N E C E T G Q H N C V P N S V C I N T R G S F Q C G P C Q P G F V G D Q A S G C Q R R A Q R F C P D G S P S E C H E H A D C V L E R D G S R S C V C A V G W A G N G I L C G R D T D L D G F P D E K L R C P E R Q C R K D N C V T V P N S G Q E D V D R D G I G D A C D P D A D G D G V P N E K D N C P L V R N P D Q R N T D E D K W G D A C D N C R S Q K N D D Q K D T D Q D G R G D A C D D D I D G D R I R N Q A D N C P R V P N S D Q K D S D G D G I G D A C D N C P Q K S N P D Q A D V D H D F V G D A C D S D Q D Q D G D G H Q D S R D N C P T V P N S A Q E D S D H D G Q G D A C D D D D D N D G V P D S R D N C R L V P N P G Q E D A D R D G V G D V C Q D D F D A D K V V D K I D V C P E N A E V T L T D F R A F Q T V V L D P E G D A Q I D P N W V V L N Q G R E I V Q T M N S D P G L A V G Y T A F N G V D F E G T F H V N T V T D D D Y A G F I F G Y Q D S S S F Y V V M W K Q M E Q T Y W Q A N P F R A V A E P G I Q L K A V K S S T G P G E Q L R N A L W H T G D T E S Q V R L L W K D P R N V G W K D K K S Y R W F L Q H R P Q V G Y I R V R F Y E G P E L V A D S N V V L D T T M R G G R L G V F C F S Q E N I I W A N L R Y R C N D T I P E D Y E T H Q L R Q A
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes ab104358 can be used as a WB positive control in conjunction with Anti-COMP/Cartilage oligomeric matrix protein antibody ab42225. |
Associated Products
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Target data
Function
Plays a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors (PubMed:16051604, PubMed:16542502). Could play a role in the pathogenesis of osteoarthritis (PubMed:16542502). Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP) (PubMed:17993464). Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7 (By similarity).
Alternative names
Cartilage oligomeric matrix protein, COMP, Thrombospondin-5, TSP5
Recommended products
Recombinant Human COMP/Cartilage oligomeric matrix protein is a Human Full Length protein, in the 21 to 757 aa range, expressed in HEK 293, with >90% purity and suitable for SDS-PAGE, WB.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- HEK 293 cells
- Tags
- DDDDK tag N-Terminus
- Applications
- SDS-PAGE, WB
- Biologically active
- No
- Accession
- P49747-1
- Animal free
- No
- Species
- Human
- Reconstitution
- Reconstitute at 0.5 mg/mL in water
- Concentration
- Storage buffer
Constituents: 0.29% Sodium chloride, 0.242% Tris
Sequence info
Amino acid sequence
- H V D Y K D D D D K P A G Q G Q S P L G S D L G P Q M L R E L Q E T N A A L Q D V R E L L R Q Q V R E I T F L K N T V M E C D A C G M Q Q S V R T G L P S V R P L L H C A P G F C F P G V A C I Q T E S G A R C G P C P A G F T G N G S H C T D V N E C N A H P C F P R V R C I N T S P G F R C E A C P P G Y S G P T H Q G V G L A F A K A N K Q V C T D I N E C E T G Q H N C V P N S V C I N T R G S F Q C G P C Q P G F V G D Q A S G C Q R R A Q R F C P D G S P S E C H E H A D C V L E R D G S R S C V C A V G W A G N G I L C G R D T D L D G F P D E K L R C P E R Q C R K D N C V T V P N S G Q E D V D R D G I G D A C D P D A D G D G V P N E K D N C P L V R N P D Q R N T D E D K W G D A C D N C R S Q K N D D Q K D T D Q D G R G D A C D D D I D G D R I R N Q A D N C P R V P N S D Q K D S D G D G I G D A C D N C P Q K S N P D Q A D V D H D F V G D A C D S D Q D Q D G D G H Q D S R D N C P T V P N S A Q E D S D H D G Q G D A C D D D D D N D G V P D S R D N C R L V P N P G Q E D A D R D G V G D V C Q D D F D A D K V V D K I D V C P E N A E V T L T D F R A F Q T V V L D P E G D A Q I D P N W V V L N Q G R E I V Q T M N S D P G L A V G Y T A F N G V D F E G T F H V N T V T D D D Y A G F I F G Y Q D S S S F Y V V M W K Q M E Q T Y W Q A N P F R A V A E P G I Q L K A V K S S T G P G E Q L R N A L W H T G D T E S Q V R L L W K D P R N V G W K D K K S Y R W F L Q H R P Q V G Y I R V R F Y E G P E L V A D S N V V L D T T M R G G R L G V F C F S Q E N I I W A N L R Y R C N D T I P E D Y E T H Q L R Q A
- Accession
- P49747
- Protein length
- Full Length
- Predicted molecular weight
- 82.4 kDa
- Amino acids
- 21 to 757
- Nature
- Recombinant
- Tags
- DDDDK tag N-Terminus
Specifications
- Form
- Lyophilized
- Additional notes
ab104358 is filtered (0.4 µm).Purity as determined by densitometric image analysis: >90%
General info
- Function
Plays a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors (PubMed:16051604, PubMed:16542502). Could play a role in the pathogenesis of osteoarthritis (PubMed:16542502). Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP) (PubMed:17993464). Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7 (By similarity).
- Sequence similarities
Belongs to the thrombospondin family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Notes
Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.
This product was previously labelled as COMP
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Cartilage Oligomeric Matrix Protein (COMP) also known as oligomeric protein or COMP cartilage protein is a structural component found in the extracellular matrix of cartilage tissues. This matrix protein has a mass of approximately 524 kDa and is predominantly expressed in cartilage particularly hyaline cartilage. COMP forms pentameric structures which make it an important supporting element in the stability and resilience of cartilage. Researchers often use techniques like COMP ELISA to quantify its levels in biological samples.
- Biological function summary
COMP interacts with other extracellular matrix components to enhance structural integrity and function of cartilage tissue. It performs significant roles in the organization and maintenance of the matrix tethering fibrillar proteins and supporting cellular interaction. COMP is not typically part of a large complex but works closely with collagens and other matrix proteins to form a robust and flexible extracellular environment.
- Pathways
COMP participates in pathways associated with cartilage formation and turnover. It engages in chondrocyte-mediated processes that are vital for endochondral ossification and cartilage repair. Within these pathways it interacts with proteins such as collagen type II and decorin which facilitate the organization and mineralization of cartilage matrix. These interactions highlight the protein's importance in maintaining cartilage architecture and function.
- Associated diseases and disorders
COMP plays a role in chondrodysplasias and osteoarthritis. Mutations in the COMP gene can lead to skeletal disorders like pseudoachondroplasia and multiple epiphyseal dysplasia causing growth and developmental anomalies. In osteoarthritis alterations in COMP levels may serve as an indicator of cartilage degradation. COMP's relationships with proteins like aggrecan and matrilin-3 in these contexts highlight its involvement in pathologies that affect cartilage structure and health.
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2 product images
SDS-PAGE - Recombinant Human COMP/Cartilage oligomeric matrix protein (ab104358)
14% SDS-PAGE showing ab104358 at approximately 82.4kDa:
Lane 1: M.W. marker – 21, 31, 45, 66, 97 kDa.
Lane 2: reduced and boiled sample, 5μg/lane.
Lane 3: non-reduced and non-boiled sample, 5μg/lane.
Western blot - Recombinant Human COMP/Cartilage oligomeric matrix protein (ab104358)
All lanes: Western blot - Anti-COMP/Cartilage oligomeric matrix protein antibody (Anti-COMP/Cartilage oligomeric matrix protein antibody ab42225) at 1/1000 dilution
All lanes: Western blot - Recombinant Human COMP/Cartilage oligomeric matrix protein (ab104358) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 82 kDa
Exposure time: 2min
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