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AB104358

Recombinant Human COMP/Cartilage oligomeric matrix protein

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(2 Publications)

Recombinant Human COMP/Cartilage oligomeric matrix protein is a Human Full Length protein, in the 21 to 757 aa range, expressed in HEK 293 cells, with >90%, suitable for SDS-PAGE, WB.

View Alternative Names

Cartilage oligomeric matrix protein, COMP, Thrombospondin-5, TSP5

2 Images
Western blot - Recombinant Human COMP/Cartilage oligomeric matrix protein (AB104358)
  • WB

Unknown

Western blot - Recombinant Human COMP/Cartilage oligomeric matrix protein (AB104358)

All lanes:

Western blot - Anti-COMP/Cartilage oligomeric matrix protein antibody (<a href='/en-us/products/primary-antibodies/comp-cartilage-oligomeric-matrix-protein-antibody-ab42225'>ab42225</a>) at 1/1000 dilution

All lanes:

Western blot - Recombinant Human COMP/Cartilage oligomeric matrix protein (ab104358) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 82 kDa

true

Exposure time: 2min

SDS-PAGE - Recombinant Human COMP/Cartilage oligomeric matrix protein (AB104358)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human COMP/Cartilage oligomeric matrix protein (AB104358)

14% SDS-PAGE showing ab104358 at approximately 82.4kDa :
Lane 1 : M.W. marker – 21, 31, 45, 66, 97 kDa.
Lane 2 : reduced and boiled sample, 5μg/lane.
Lane 3 : non-reduced and non-boiled sample, 5μg/lane.

Key facts

Purity

>90% SDS-PAGE

Expression system

HEK 293 cells

Tags

DDDDK tag N-Terminus

Applications

SDS-PAGE, WB

applications

Biologically active

No

Accession

P49747

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute at 0.5 mg/mL in water

Storage buffer

Constituents: 0.29% Sodium chloride, 0.242% Tris

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab104358 can be used as a WB positive control in conjunction with <a href='/en-us/products/primary-antibodies/comp-cartilage-oligomeric-matrix-protein-antibody-ab42225'>ab42225</a>.</p>" } } }
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Product details

Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.

This product was previously labelled as COMP

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Sequence info

[{"sequence":"HVDYKDDDDKPAGQGQSPLGSDLGPQMLRELQETNAALQDVRELLRQQVREITFLKNTVMECDACGMQQSVRTGLPSVRPLLHCAPGFCFPGVACIQTESGARCGPCPAGFTGNGSHCTDVNECNAHPCFPRVRCINTSPGFRCEACPPGYSGPTHQGVGLAFAKANKQVCTDINECETGQHNCVPNSVCINTRGSFQCGPCQPGFVGDQASGCQRRAQRFCPDGSPSECHEHADCVLERDGSRSCVCAVGWAGNGILCGRDTDLDGFPDEKLRCPERQCRKDNCVTVPNSGQEDVDRDGIGDACDPDADGDGVPNEKDNCPLVRNPDQRNTDEDKWGDACDNCRSQKNDDQKDTDQDGRGDACDDDIDGDRIRNQADNCPRVPNSDQKDSDGDGIGDACDNCPQKSNPDQADVDHDFVGDACDSDQDQDGDGHQDSRDNCPTVPNSAQEDSDHDGQGDACDDDDDNDGVPDSRDNCRLVPNPGQEDADRDGVGDVCQDDFDADKVVDKIDVCPENAEVTLTDFRAFQTVVLDPEGDAQIDPNWVVLNQGREIVQTMNSDPGLAVGYTAFNGVDFEGTFHVNTVTDDDYAGFIFGYQDSSSFYVVMWKQMEQTYWQANPFRAVAEPGIQLKAVKSSTGPGEQLRNALWHTGDTESQVRLLWKDPRNVGWKDKKSYRWFLQHRPQVGYIRVRFYEGPELVADSNVVLDTTMRGGRLGVFCFSQENIIWANLRYRCNDTIPEDYETHQLRQA","proteinLength":"Full Length","predictedMolecularWeight":"82.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":757,"aminoAcidStart":21,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P49747","tags":[{"tag":"DDDDK","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Cartilage Oligomeric Matrix Protein (COMP) also known as oligomeric protein or COMP cartilage protein is a structural component found in the extracellular matrix of cartilage tissues. This matrix protein has a mass of approximately 524 kDa and is predominantly expressed in cartilage particularly hyaline cartilage. COMP forms pentameric structures which make it an important supporting element in the stability and resilience of cartilage. Researchers often use techniques like COMP ELISA to quantify its levels in biological samples.
Biological function summary

COMP interacts with other extracellular matrix components to enhance structural integrity and function of cartilage tissue. It performs significant roles in the organization and maintenance of the matrix tethering fibrillar proteins and supporting cellular interaction. COMP is not typically part of a large complex but works closely with collagens and other matrix proteins to form a robust and flexible extracellular environment.

Pathways

COMP participates in pathways associated with cartilage formation and turnover. It engages in chondrocyte-mediated processes that are vital for endochondral ossification and cartilage repair. Within these pathways it interacts with proteins such as collagen type II and decorin which facilitate the organization and mineralization of cartilage matrix. These interactions highlight the protein's importance in maintaining cartilage architecture and function.

COMP plays a role in chondrodysplasias and osteoarthritis. Mutations in the COMP gene can lead to skeletal disorders like pseudoachondroplasia and multiple epiphyseal dysplasia causing growth and developmental anomalies. In osteoarthritis alterations in COMP levels may serve as an indicator of cartilage degradation. COMP's relationships with proteins like aggrecan and matrilin-3 in these contexts highlight its involvement in pathologies that affect cartilage structure and health.
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Specifications

Form

Lyophilized

Additional notes

ab104358 is filtered (0.4 μm).Purity as determined by densitometric image analysis: >90%

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General info

Function

Plays a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors (PubMed : 16051604, PubMed : 16542502). Could play a role in the pathogenesis of osteoarthritis (PubMed : 16542502). Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP) (PubMed : 17993464). Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7 (By similarity).

Sequence similarities

Belongs to the thrombospondin family.

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Product protocols

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Target data

Plays a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors (PubMed : 16051604, PubMed : 16542502). Could play a role in the pathogenesis of osteoarthritis (PubMed : 16542502). Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP) (PubMed : 17993464). Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7 (By similarity).
See full target information COMP
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of cell biology 213:305-14 PubMed27138253

2016

Secretory cargo sorting by Ca2+-dependent Cab45 oligomerization at the trans-Golgi network.

Applications

Unspecified application

Species

Unspecified reactive species

Alvaro H Crevenna,Birgit Blank,Andreas Maiser,Derya Emin,Jens Prescher,Gisela Beck,Christine Kienzle,Kira Bartnik,Bianca Habermann,Mehrshad Pakdel,Heinrich Leonhardt,Don C Lamb,Julia von Blume

American journal of human genetics 92:366-74 PubMed23415669

2013

ADAMTS7 cleavage and vascular smooth muscle cell migration is affected by a coronary-artery-disease-associated variant.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangyuan Pu,Qingzhong Xiao,Stefan Kiechl,Kenneth Chan,Fu Liang Ng,Shivani Gor,Robin N Poston,Changcun Fang,Ashish Patel,Ece C Senver,Sue Shaw-Hawkins,Johann Willeit,Chuanju Liu,Jianhua Zhu,Arthur T Tucker,Qingbo Xu,Mark J Caulfield,Shu Ye
View all publications
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Complementary Products

Primary Antibodies

AB300555

Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (AB300555)

  • 0
  • 0
Assay Kits

AB275907

Human COMP ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human COMP ELISA Kit (AB275907)

  • 0
  • 0
Secondary Antibodies

AB125912

Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L

RabMAb|Recombinant

ELISA - Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L (AB125912)

  • 0
  • 0
Proteins & Peptides

AB288799

Recombinant Human VCAM1 protein (Active)

Premium Bioactive

Biological Activity - Recombinant Human VCAM1 protein (Active) (AB288799)

  • 0
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