Recombinant human COMP/Cartilage oligomeric matrix protein (Active) is a Human Full Length protein, in the 21 to 757 aa range, expressed in HEK 293, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
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- American journal of physiology. Lung cellular and molecular physiology 323:L400-L4092022Endothelin-1 depletion of cartilage oligomeric matrix protein modulates pulmonary artery superoxide and iron metabolism-associated mitochondrial heme biosynthesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHang Yu,Norah Alruwaili,Melissa R Kelly,Bin Zhang,Aijing Liu,Yingqi Wang,Dong Sun,Michael S WolinPubMed 35943724
- American journal of physiology. Lung cellular and 317:L569-L5772019Potential role of cartilage oligomeric matrix protein in the modulation of pulmonary arterial smooth muscle superoxide by hypoxia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHang Yu et. al.PubMed 31389735
Key facts
- Purity
>90% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
HEK 293 cells
- Tags
His tag C-Terminus
- Applications
SDS-PAGE, FuncS
- Biologically active
Yes
Amino acid sequence
Q G Q S P L G S D L G P Q M L R E L Q E T N A A L Q D V R E L L R Q Q V R E I T F L K N T V M E C D A C G M Q Q S V R T G L P S V R P L L H C A P G F C F P G V A C I Q T E S G A R C G P C P A G F T G N G S H C T D V N E C N A H P C F P R V R C I N T S P G F R C E A C P P G Y S G P T H Q G V G L A F A K A N K Q V C T D I N E C E T G Q H N C V P N S V C I N T R G S F Q C G P C Q P G F V G D Q A S G C Q R R A Q R F C P D G S P S E C H E H A D C V L E R D G S R S C V C A V G W A G N G I L C G R D T D L D G F P D E K L R C P E R Q C R K D N C V T V P N S G Q E D V D R D G I G D A C D P D A D G D G V P N E K D N C P L V R N P D Q R N T D E D K W G D A C D N C R S Q K N D D Q K D T D Q D G R G D A C D D D I D G D R I R N Q A D N C P R V P N S D Q K D S D G D G I G D A C D N C P Q K S N P D Q A D V D H D F V G D A C D S D Q D Q D G D G H Q D S R D N C P T V P N S A Q E D S D H D G Q G D A C D D D D D N D G V P D S R D N C R L V P N P G Q E D A D R D G V G D V C Q D D F D A D K V V D K I D V C P E N A E V T L T D F R A F Q T V V L D P E G D A Q I D P N W V V L N Q G R E I V Q T M N S D P G L A V G Y T A F N G V D F E G T F H V N T V T D D D Y A G F I F G Y Q D S S S F Y V V M W K Q M E Q T Y W Q A N P F R A V A E P G I Q L K A V K S S T G P G E Q L R N A L W H T G D T E S Q V R L L W K D P R N V G W K D K K S Y R W F L Q H R P Q V G Y I R V R F Y E G P E L V A D S N V V L D T T M R G G R L G V F C F S Q E N I I W A N L R Y R C N D T I P E D Y E T H Q L R Q A
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Plays a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors (PubMed:16051604, PubMed:16542502). Could play a role in the pathogenesis of osteoarthritis (PubMed:16542502). Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP) (PubMed:17993464). Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7 (By similarity).
Alternative names
Cartilage oligomeric matrix protein, COMP, Thrombospondin-5, TSP5, COMP
Recommended products
Recombinant human COMP/Cartilage oligomeric matrix protein (Active) is a Human Full Length protein, in the 21 to 757 aa range, expressed in HEK 293, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
>90% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
HEK 293 cells
- Tags
His tag C-Terminus
- Applications
SDS-PAGE, FuncS
- Biologically active
Yes
- Biological activity
Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells, when COMP / Thrombospondin‑5 immobilized at 1 μg/well.
More than 45% of ATDC-5 cell adhesion will be induced.
- Accession
- P49747-1
- Animal free
No
- Species
Human
- Reconstitution
Reconstitute with sterile deionized water to a concentration of 200 µg/ml.
- Concentration
- Storage buffer
pH: 7.4
Constituents: 3% Trehalose, 0.6% Tris, 0.58% Sodium chloride
Sequence info
Amino acid sequence
Q G Q S P L G S D L G P Q M L R E L Q E T N A A L Q D V R E L L R Q Q V R E I T F L K N T V M E C D A C G M Q Q S V R T G L P S V R P L L H C A P G F C F P G V A C I Q T E S G A R C G P C P A G F T G N G S H C T D V N E C N A H P C F P R V R C I N T S P G F R C E A C P P G Y S G P T H Q G V G L A F A K A N K Q V C T D I N E C E T G Q H N C V P N S V C I N T R G S F Q C G P C Q P G F V G D Q A S G C Q R R A Q R F C P D G S P S E C H E H A D C V L E R D G S R S C V C A V G W A G N G I L C G R D T D L D G F P D E K L R C P E R Q C R K D N C V T V P N S G Q E D V D R D G I G D A C D P D A D G D G V P N E K D N C P L V R N P D Q R N T D E D K W G D A C D N C R S Q K N D D Q K D T D Q D G R G D A C D D D I D G D R I R N Q A D N C P R V P N S D Q K D S D G D G I G D A C D N C P Q K S N P D Q A D V D H D F V G D A C D S D Q D Q D G D G H Q D S R D N C P T V P N S A Q E D S D H D G Q G D A C D D D D D N D G V P D S R D N C R L V P N P G Q E D A D R D G V G D V C Q D D F D A D K V V D K I D V C P E N A E V T L T D F R A F Q T V V L D P E G D A Q I D P N W V V L N Q G R E I V Q T M N S D P G L A V G Y T A F N G V D F E G T F H V N T V T D D D Y A G F I F G Y Q D S S S F Y V V M W K Q M E Q T Y W Q A N P F R A V A E P G I Q L K A V K S S T G P G E Q L R N A L W H T G D T E S Q V R L L W K D P R N V G W K D K K S Y R W F L Q H R P Q V G Y I R V R F Y E G P E L V A D S N V V L D T T M R G G R L G V F C F S Q E N I I W A N L R Y R C N D T I P E D Y E T H Q L R Q A
- Accession
- Protein length
Full Length
- Predicted molecular weight
82 kDa
- Amino acids
21 to 757
- Nature
Recombinant
- Tags
His tag C-Terminus
Specifications
- Form
Lyophilized
General info
- Function
Plays a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors (PubMed:16051604, PubMed:16542502). Could play a role in the pathogenesis of osteoarthritis (PubMed:16542502). Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP) (PubMed:17993464). Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7 (By similarity).
- Sequence similarities
Belongs to the thrombospondin family.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon reconstitution add a carrier protein (0.1% BSA)
- Storage information
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
This product was previously labelled as COMP
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Cartilage Oligomeric Matrix Protein (COMP) also known as oligomeric protein or COMP cartilage protein is a structural component found in the extracellular matrix of cartilage tissues. This matrix protein has a mass of approximately 524 kDa and is predominantly expressed in cartilage particularly hyaline cartilage. COMP forms pentameric structures which make it an important supporting element in the stability and resilience of cartilage. Researchers often use techniques like COMP ELISA to quantify its levels in biological samples.
- Biological function summary
COMP interacts with other extracellular matrix components to enhance structural integrity and function of cartilage tissue. It performs significant roles in the organization and maintenance of the matrix tethering fibrillar proteins and supporting cellular interaction. COMP is not typically part of a large complex but works closely with collagens and other matrix proteins to form a robust and flexible extracellular environment.
- Pathways
COMP participates in pathways associated with cartilage formation and turnover. It engages in chondrocyte-mediated processes that are vital for endochondral ossification and cartilage repair. Within these pathways it interacts with proteins such as collagen type II and decorin which facilitate the organization and mineralization of cartilage matrix. These interactions highlight the protein's importance in maintaining cartilage architecture and function.
- Associated diseases and disorders
COMP plays a role in chondrodysplasias and osteoarthritis. Mutations in the COMP gene can lead to skeletal disorders like pseudoachondroplasia and multiple epiphyseal dysplasia causing growth and developmental anomalies. In osteoarthritis alterations in COMP levels may serve as an indicator of cartilage degradation. COMP's relationships with proteins like aggrecan and matrilin-3 in these contexts highlight its involvement in pathologies that affect cartilage structure and health.
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1 product image
SDS-PAGE - Recombinant human COMP/Cartilage oligomeric matrix protein (Active) (ab174082)
SDS-PAGE analysis of ab174082 purity. Note: DTT-reduced Protein migrates as 110-130 kDa due to glycosylation.
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