Recombinant Human COMP Protein Standard (His tag)
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Recombinant Human COMP Protein Standard (His tag) is a Human Fragment protein, expressed in HEK 293 cells, with >80%, suitable for SDS-PAGE, sELISA.
View Alternative Names
Cartilage oligomeric matrix protein, COMP, Thrombospondin-5, TSP5
- sELISA
Supplier Data
Sandwich ELISA - Recombinant Human COMP Protein Standard (His tag) (AB316616)
Sandwich ELISA with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant Human COMP Protein Standard (His tag) (AB316616)
SDS-PAGE analysis of ab316616 under reducing conditions for 2ug protein.
Reactivity data
Product details
While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.
Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.
The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
COMP interacts with other extracellular matrix components to enhance structural integrity and function of cartilage tissue. It performs significant roles in the organization and maintenance of the matrix tethering fibrillar proteins and supporting cellular interaction. COMP is not typically part of a large complex but works closely with collagens and other matrix proteins to form a robust and flexible extracellular environment.
Pathways
COMP participates in pathways associated with cartilage formation and turnover. It engages in chondrocyte-mediated processes that are vital for endochondral ossification and cartilage repair. Within these pathways it interacts with proteins such as collagen type II and decorin which facilitate the organization and mineralization of cartilage matrix. These interactions highlight the protein's importance in maintaining cartilage architecture and function.
Specifications
Form
Liquid
General info
Function
Plays a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors (PubMed : 16051604, PubMed : 16542502). Could play a role in the pathogenesis of osteoarthritis (PubMed : 16542502). Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP) (PubMed : 17993464). Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7 (By similarity).
Sequence similarities
Belongs to the thrombospondin family.
Target data
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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