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AB316488

Recombinant Human Complement C2 Protein Standard (His tag)

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Recombinant Human Complement C2 Protein Standard (His tag) is a Human Full Length protein, expressed in HEK 293 cells, with >80%, suitable for SDS-PAGE, sELISA.
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Sandwich ELISA - Recombinant Human Complement C2 Protein Standard (His tag) (AB316488)
  • sELISA

Supplier Data

Sandwich ELISA - Recombinant Human Complement C2 Protein Standard (His tag) (AB316488)

Sandwich ELISA with the capture antibody ab259713 dilution at 2 μg/mL and detector antibody ab259714 dilution at 0.5 μg/mL after biotin conjugation.

SDS-PAGE - Recombinant Human Complement C2 Protein Standard (His tag) (AB316488)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Complement C2 Protein Standard (His tag) (AB316488)

SDS-PAGE analysis of ab316488 under reducing conditions for 2ug protein.

Key facts

Purity

>80% SDS-PAGE

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

SDS-PAGE, sELISA

applications

Biologically active

No

Accession

P06681-1

Animal free

Yes

Carrier free

No

Species

Human

Storage buffer

pH: 7.3 - 7.5 Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic

storage-buffer

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Product details

While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.

Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.

The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.

Sequence info

[{"linker":null,"sequence":null,"proteinLength":"Full Length","predictedMolecularWeight":"82.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P06681","tags":[{"tag":"His","terminus":"C-Terminus"}]}]

Properties and storage information

Form
Liquid
Purification technique
Affinity purification His Tag
Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False

Product protocols

Target data

Component C2 which is part of the classical pathway of the complement system is cleaved by activated factor C1 into two fragments : C2b and C2a. C2a, a serine protease, then combines with complement factor C4b to generate the C3 or C5 convertase.
See full target information C2

Alternative Names

Complement C2, C3/C5 convertase, C2

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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