Recombinant Human Complement Factor B (Ba fragment) Protein Standard
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Recombinant Human Complement Factor B (Ba fragment) Protein Standard is a Human Fragment protein, expressed in HEK 293 cells, with >80%, suitable for SDS-PAGE, sELISA.
View Alternative Names
BF, BFD, CFB, Complement factor B, C3/C5 convertase, Glycine-rich beta glycoprotein, PBF2, Properdin factor B, GBG
- sELISA
Supplier Data
Sandwich ELISA - Recombinant Human Complement Factor B (Ba fragment) Protein Standard (AB322695)
Sandwich ELISA with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant Human Complement Factor B (Ba fragment) Protein Standard (AB322695)
SDS-PAGE analysis of ab322695 under reducing conditions for 2ug protein.
Complementary Products
Reagents, Controls & Accessories
AB234617
Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa)
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Reactivity data
Product details
While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.
Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.
The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Supportteam to know which antibody pair is suitable for this protein.
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Specifications
Form
Liquid
General info
Function
Precursor of the catalytic component of the C3 and C5 convertase complexes of the alternative pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed : 3638964, PubMed : 624565, PubMed : 6554279, PubMed : 6919543, PubMed : 9748277). The alternative complement pathway acts as an amplification loop that enhances other complement pathways (classical, lectin and GZMK) by promoting formation of additional C3 and C5 convertases (PubMed : 3638964, PubMed : 624565, PubMed : 6554279, PubMed : 6919543, PubMed : 9748277). CFB is cleaved and activated by CFD to generate Ba and Bb chains; Bb chain constituting the catalytic component of the C3 and C5 convertases (PubMed : 6769474, PubMed : 9748277).. Complement factor B Bb. Serine protease component of the complement C3 and C5 convertase complexes of the alternative complement pathway (PubMed : 30643019, PubMed : 3638964, PubMed : 624565, PubMed : 6554279, PubMed : 6919543). Following cleavage and activation by factor D (CFD), forms the C3 convertase together with complement C3b (PubMed : 3638964, PubMed : 624565, PubMed : 6554279, PubMed : 6919543, PubMed : 9748277). As part of the C3 convertase, cleaves and activates C3 into C3a anaphylatoxin and C3b opsonin, the next components of the complement pathways (PubMed : 3638964, PubMed : 624565, PubMed : 6554279, PubMed : 6919543, PubMed : 9748277). When an additional complement C3b molecule binds to the C3 convertase, forms the C5 convertase, which cleaves and activates C5 into C5a anaphylatoxin and C5b component of the membrane attack complex (PubMed : 30643019, PubMed : 624565, PubMed : 6554279).. Complement factor B Ba. Involved in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes.
Sequence similarities
Belongs to the peptidase S1 family.
Post-translational modifications
Cleaved by CFD following activation of the alternative complement system, generating Ba and Bb chains (PubMed:21205667, PubMed:6769474, PubMed:874324, PubMed:9748277). Cleavage and activation takes place when CFB is already associated with complement C3b (PubMed:21205667).
Target data
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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