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AB174401

Recombinant Human Complement factor B protein (denatured) (His tag N-Terminus)

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Recombinant Human Complement factor B protein (denatured) (His tag N-Terminus) is a Human Fragment protein, in the 260 to 764 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

BF, BFD, CFB, Complement factor B, C3/C5 convertase, Glycine-rich beta glycoprotein, PBF2, Properdin factor B, GBG

1 Images
SDS-PAGE - Recombinant Human Complement factor B protein (denatured) (His tag N-Terminus) (AB174401)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Complement factor B protein (denatured) (His tag N-Terminus) (AB174401)

15% SDS-PAGE analysis of ab174401 (3μg).

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P00751

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 2.4% Urea, 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSKIVLDPSGSMNIYLVLDGSDSIGASNFTGAKKCLVNLIEKVASYGVKPRYGLVTYATYPKIWVKVSEADSSNADWVTKQLNEINYEDHKLKSGTNTKKALQAVYSMMSWPDDVPPEGWNRTRHVIILMTDGLHNMGGDPITVIDEIRDLLYIGKDRKNPREDYLDVYVFGVGPLVNQVNINALASKKDNEQHVFKVKDMENLEDVFYQMIDESQSLSLCGMVWEHRKGTDYHKQPWQAKISVIRPSKGHESCMGAVVSEYFVLTAAHCFTVDDKEHSIKVSVGGEKRDLEIEVVLFHPNYNINGKKEAGIPEFYDYDVALIKLKNKLKYGQTIRPICLPCTEGTTRALRLPPTTTCQQQKEELLPAQDIKALFVSEEEKKLTRKEVYIKNGDKKGSCERDAQYAPGYDKVKDISEVVTPRFLCTGGVSPYADPNTCRGDSGGPLIVHKRSRFIQVGVISWGVVDVCKNQKRQKQVPAHARDFHINLFQVLPWLKEKLQDEDLGFL","proteinLength":"Fragment","predictedMolecularWeight":"59.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":764,"aminoAcidStart":260,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P00751","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Complement factor B also known as CFB protein is a component of the complement system. It plays an important role in the alternative pathway. This protein with a molecular mass of approximately 93 kDa is produced mainly in the liver and expresses in various tissues throughout the body. Factor B comprises several domains including the von Willebrand factor type A domain essential for its activity. CFB engages in protein-protein interactions important for the function of the complement cascade.
Biological function summary

Complement factor B is essential in the immune system. It participates in the formation of the C3 convertase complex which is an important step in the amplification of the complement cascade. This protein contributes to the opsonization and clearance of pathogens assisting in the immune response. CFB binds with complement component C3b to participate in the alternative pathway which does not require antibody presence for activation.

Pathways

Complement factor B is part of the innate immune pathway. It helps mediate the alternative complement pathway acting as an amplifier of immune responses. This pathway interacts closely with the classical and lectin pathways ensuring a versatile defense mechanism. Factor D for example also plays a complementary role by cleaving factor B when bound to C3b to form C3 convertase a critical step in propagating the immune response.

Complement factor B associates with kidney-related diseases like atypical hemolytic uremic syndrome (aHUS). Mutations in CFB can lead to deregulated complement activation contributing to pathological conditions like age-related macular degeneration (AMD). The relationship with protein C3 is important in these disorders as both factor B and C3 engage in interactions that when dysfunctional influence disease outcomes. Understanding these interactions can help develop targeted therapies such as anti-complement agents to modulate the complement system in these diseases.
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Specifications

Form

Liquid

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General info

Function

Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments : Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase. It has also been implicated in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes. Ba inhibits the proliferation of preactivated B-lymphocytes.

Sequence similarities

Belongs to the peptidase S1 family.

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Product protocols

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Target data

Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments : Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase. It has also been implicated in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes. Ba inhibits the proliferation of preactivated B-lymphocytes.
See full target information CFB
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