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AB276729

Recombinant Human Complement factor B protein (His tag)

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Recombinant Human Complement factor B protein (His tag) is a Human Full Length protein, in the 1 to 764 aa range, expressed in HEK 293 cells, with >90%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE.

View Alternative Names

BF, BFD, CFB, Complement factor B, C3/C5 convertase, Glycine-rich beta glycoprotein, PBF2, Properdin factor B, GBG

1 Images
SDS-PAGE - Recombinant Human Complement factor B protein (His tag) (AB276729)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Complement factor B protein (His tag) (AB276729)

SDS-PAGE analysis of ab276729

Key facts

Purity

>90% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P00751

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: PBS

storage-buffer

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Complementary Products

Reagents, Controls & Accessories

AB116029

Prestained Protein Ladder - Extra broad molecular weight (5 - 245 kDa)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (5 - 245 kDa) (AB116029)

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Assay Kits

AB300318

Human Factor B ELISA Kit

Recombinant|SimpleStep

ELISA - Human Factor B ELISA Kit (AB300318)

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  • 1
Proteins & Peptides

AB276997

Recombinant Mouse Factor H protein (His tag)

SDS-PAGE - Recombinant Mouse Factor H protein (His tag) (AB276997)

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Primary Antibodies

AB309149

Anti-Complement factor B antibody [EPR27443-6]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Complement factor B antibody [EPR27443-6] (AB309149)

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  • 0
Assay Kits

AB264609

Mouse Complement C5 ELISA Kit

Recombinant|SimpleStep

ELISA - Mouse Complement C5 ELISA Kit (AB264609)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"linker":null,"sequence":"MGSNLSPQLCLMPFILGLLSGGVTTTPWSLARPQGSCSLEGVEIKGGSFRLLQEGQALEYVCPSGFYPYPVQTRTCRSTGSWSTLKTQDQKTVRKAECRAIHCPRPHDFENGEYWPRSPYYNVSDEISFHCYDGYTLRGSANRTCQVNGRWSGQTAICDNGAGYCSNPGIPIGTRKVGSQYRLEDSVTYHCSRGLTLRGSQRRTCQEGGSWSGTEPSCQDSFMYDTPQEVAEAFLSSLTETIEGVDAEDGHGPGEQQKRKIVLDPSGSMNIYLVLDGSDSIGASNFTGAKKCLVNLIEKVASYGVKPRYGLVTYATYPKIWVKVSEADSSNADWVTKQLNEINYEDHKLKSGTNTKKALQAVYSMMSWPDDVPPEGWNRTRHVIILMTDGLHNMGGDPITVIDEIRDLLYIGKDRKNPREDYLDVYVFGVGPLVNQVNINALASKKDNEQHVFKVKDMENLEDVFYQMIDESQSLSLCGMVWEHRKGTDYHKQPWQAKISVIRPSKGHESCMGAVVSEYFVLTAAHCFTVDDKEHSIKVSVGGEKRDLEIEVVLFHPNYNINGKKEAGIPEFYDYDVALIKLKNKLKYGQTIRPICLPCTEGTTRALRLPPTTTCQQQKEELLPAQDIKALFVSEEEKKLTRKEVYIKNGDKKGSCERDAQYAPGYDKVKDISEVVTPRFLCTGGVSPYADPNTCRGDSGGPLIVHKRSRFIQVGVISWGVVDVCKNQKRQKQVPAHARDFHINLFQVLPWLKEKLQDEDLGFL","proteinLength":"Full Length","predictedMolecularWeight":"84.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":764,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P00751","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Form
Lyophilized
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Complement factor B also known as CFB protein is a component of the complement system. It plays an important role in the alternative pathway. This protein with a molecular mass of approximately 93 kDa is produced mainly in the liver and expresses in various tissues throughout the body. Factor B comprises several domains including the von Willebrand factor type A domain essential for its activity. CFB engages in protein-protein interactions important for the function of the complement cascade.
Biological function summary

Complement factor B is essential in the immune system. It participates in the formation of the C3 convertase complex which is an important step in the amplification of the complement cascade. This protein contributes to the opsonization and clearance of pathogens assisting in the immune response. CFB binds with complement component C3b to participate in the alternative pathway which does not require antibody presence for activation.

Pathways

Complement factor B is part of the innate immune pathway. It helps mediate the alternative complement pathway acting as an amplifier of immune responses. This pathway interacts closely with the classical and lectin pathways ensuring a versatile defense mechanism. Factor D for example also plays a complementary role by cleaving factor B when bound to C3b to form C3 convertase a critical step in propagating the immune response.

Complement factor B associates with kidney-related diseases like atypical hemolytic uremic syndrome (aHUS). Mutations in CFB can lead to deregulated complement activation contributing to pathological conditions like age-related macular degeneration (AMD). The relationship with protein C3 is important in these disorders as both factor B and C3 engage in interactions that when dysfunctional influence disease outcomes. Understanding these interactions can help develop targeted therapies such as anti-complement agents to modulate the complement system in these diseases.
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General info

Function

Precursor of the catalytic component of the C3 and C5 convertase complexes of the alternative pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed : 3638964, PubMed : 624565, PubMed : 6554279, PubMed : 6919543, PubMed : 9748277). The alternative complement pathway acts as an amplification loop that enhances other complement pathways (classical, lectin and GZMK) by promoting formation of additional C3 and C5 convertases (PubMed : 3638964, PubMed : 624565, PubMed : 6554279, PubMed : 6919543, PubMed : 9748277). CFB is cleaved and activated by CFD to generate Ba and Bb chains; Bb chain constituting the catalytic component of the C3 and C5 convertases (PubMed : 6769474, PubMed : 9748277).. Complement factor B Bb. Serine protease component of the complement C3 and C5 convertase complexes of the alternative complement pathway (PubMed : 30643019, PubMed : 3638964, PubMed : 624565, PubMed : 6554279, PubMed : 6919543). Following cleavage and activation by factor D (CFD), forms the C3 convertase together with complement C3b (PubMed : 3638964, PubMed : 624565, PubMed : 6554279, PubMed : 6919543, PubMed : 9748277). As part of the C3 convertase, cleaves and activates C3 into C3a anaphylatoxin and C3b opsonin, the next components of the complement pathways (PubMed : 3638964, PubMed : 624565, PubMed : 6554279, PubMed : 6919543, PubMed : 9748277). When an additional complement C3b molecule binds to the C3 convertase, forms the C5 convertase, which cleaves and activates C5 into C5a anaphylatoxin and C5b component of the membrane attack complex (PubMed : 30643019, PubMed : 624565, PubMed : 6554279).. Complement factor B Ba. Involved in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes.

Sequence similarities

Belongs to the peptidase S1 family.

Post-translational modifications

Cleaved by CFD following activation of the alternative complement system, generating Ba and Bb chains (PubMed:21205667, PubMed:6769474, PubMed:874324, PubMed:9748277). Cleavage and activation takes place when CFB is already associated with complement C3b (PubMed:21205667).

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Product protocols

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Target data

Precursor of the catalytic component of the C3 and C5 convertase complexes of the alternative pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed : 3638964, PubMed : 624565, PubMed : 6554279, PubMed : 6919543, PubMed : 9748277). The alternative complement pathway acts as an amplification loop that enhances other complement pathways (classical, lectin and GZMK) by promoting formation of additional C3 and C5 convertases (PubMed : 3638964, PubMed : 624565, PubMed : 6554279, PubMed : 6919543, PubMed : 9748277). CFB is cleaved and activated by CFD to generate Ba and Bb chains; Bb chain constituting the catalytic component of the C3 and C5 convertases (PubMed : 6769474, PubMed : 9748277).. Complement factor B Bb. Serine protease component of the complement C3 and C5 convertase complexes of the alternative complement pathway (PubMed : 30643019, PubMed : 3638964, PubMed : 624565, PubMed : 6554279, PubMed : 6919543). Following cleavage and activation by factor D (CFD), forms the C3 convertase together with complement C3b (PubMed : 3638964, PubMed : 624565, PubMed : 6554279, PubMed : 6919543, PubMed : 9748277). As part of the C3 convertase, cleaves and activates C3 into C3a anaphylatoxin and C3b opsonin, the next components of the complement pathways (PubMed : 3638964, PubMed : 624565, PubMed : 6554279, PubMed : 6919543, PubMed : 9748277). When an additional complement C3b molecule binds to the C3 convertase, forms the C5 convertase, which cleaves and activates C5 into C5a anaphylatoxin and C5b component of the membrane attack complex (PubMed : 30643019, PubMed : 624565, PubMed : 6554279).. Complement factor B Ba. Involved in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes.
See full target information CFB
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