Recombinant human COMT protein (Active) is a Human Fragment protein, in the 27 to 271 aa range, expressed in Baculovirus infected Sf9, with >95% purity and suitable for SDS-PAGE, FuncS.
Images
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
R H W G W G L C L I G W N E F I L Q P I H N L L M G D T K E Q R I L N H V L Q H A E P G N A Q S V L E A I D T Y C E Q K E W A M N V G D K K G K I V D A V I Q E H Q P S V L L E L G A Y C G Y S A V R M A R L L S P G A R L I T I E I N P D C A A I T Q R M V D F A G V K D K V T L V V G A S Q D I I P Q L K K K Y D V D T L D M V F L D H W K D R Y L P D T L L L E E C G L L R K G T V L L A D N V I C P G A P D F L A H V R G S S C F E C T H Y Q S F L E Y R E V V D G L E K A I Y K G P G S E A G P
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.
Alternative names
Catechol O-methyltransferase, COMT
Recommended products
Recombinant human COMT protein (Active) is a Human Fragment protein, in the 27 to 271 aa range, expressed in Baculovirus infected Sf9, with >95% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- The specific activity of ab268426 was 160 nmol/min/mg in a methyltransferase assay using dopamine hydrochloride as substrate.
- Accession
- P21964-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence
- R H W G W G L C L I G W N E F I L Q P I H N L L M G D T K E Q R I L N H V L Q H A E P G N A Q S V L E A I D T Y C E Q K E W A M N V G D K K G K I V D A V I Q E H Q P S V L L E L G A Y C G Y S A V R M A R L L S P G A R L I T I E I N P D C A A I T Q R M V D F A G V K D K V T L V V G A S Q D I I P Q L K K K Y D V D T L D M V F L D H W K D R Y L P D T L L L E E C G L L R K G T V L L A D N V I C P G A P D F L A H V R G S S C F E C T H Y Q S F L E Y R E V V D G L E K A I Y K G P G S E A G P
- Accession
- P21964
- Protein length
- Fragment
- Amino acids
- 27 to 271
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.
- Sequence similarities
Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-dependent O-methyltransferase family.
- Post-translational modifications
The N-terminus is blocked.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
COMT also known as catechol-O-methyltransferase is an enzyme responsible for the methylation of catechol compounds including neurotransmitters like dopamine epinephrine and norepinephrine. This reaction requires S-adenosylmethionine as a methyl donor. COMT primarily has two forms: a soluble form and a membrane-bound form with masses around 24 kDa and 30 kDa respectively. This protein shows significant expression in the liver brain and kidneys. COMT expression is also noted in cell lines such as HEK293T where it plays an important role in catecholamine metabolism.
- Biological function summary
Catechol-O-methyltransferase serves as a regulator of neurotransmitter levels in the central nervous system and peripheral tissues. It does not appear to form part of a larger protein complex functioning mainly as a singular entity for metabolizing catechols. Through its action COMT impacts processes such as mood regulation cognition and stress response. Its role in degrading dopamine is especially significant in regions like the prefrontal cortex where dopamine plays an important role in executive functions.
- Pathways
COMT participates in the catecholamine degradation pathway and impacts dopaminergic signaling. It works alongside enzymes such as monoamine oxidase (MAO) to regulate levels of neurotransmitters. COMT's activity influences the synaptic presence of dopamine and its interaction in signaling pathways. Moreover it indirectly affects the balance within the dopaminergic system which is important for normal neurological function and behavior.
- Associated diseases and disorders
COMT has links to psychiatric and neurological conditions such as schizophrenia and Parkinson’s disease. Variations in COMT function can affect dopamine metabolism which may contribute to the etiology of these disorders. Additionally proteins like MAO and dopamine transporter (DAT) also connect to COMT through their shared involvement in neurotransmitter regulation. Alterations in COMT activity can influence disease severity and patient response to treatment underlining its importance in understanding these disorders.
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2 product images
Functional Studies - Recombinant human COMT protein (Active) (ab268426)
The specific activity of ab268426 was 160 nmol/min/mg in a methyltransferase assay using dopamine hydrochloride as substrate.
SDS-PAGE - Recombinant human COMT protein (Active) (ab268426)
SDS-PAGE analysis of ab268426.
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