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AB272107

Recombinant Human coronavirus SARS-CoV-2 Nucleocapsid Protein (His tag)

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(2 Publications)

Recombinant Human coronavirus SARS-CoV-2 Nucleocapsid Protein (His tag) is a SARS-CoV Full Length protein, in the 1 to 419 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE.

View Alternative Names

9a, N, Nucleoprotein, Nucleocapsid protein, NC, Protein N

3 Images
Western blot - Recombinant Human coronavirus SARS-CoV-2 Nucleocapsid Protein (His tag) (AB272107)
  • WB

Lab

Western blot - Recombinant Human coronavirus SARS-CoV-2 Nucleocapsid Protein (His tag) (AB272107)

**Lanes 1 - 3 : ** Green - ab271180 observed at 51 kDa.** Lanes 4 - 6 : ** Red - loading control ab18184 (Mouse monoclonal [HIS.H8] to 6X His tag®) observed at 51 kDa. ab271180 was shown to react with SARS-CoV-2 nucleocapsid protein in Western blot. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab271180 and ab18184 (Mouse monoclonal [HIS.H8] to 6X His tag®) overnight at 4 °C at a 1 in 1000 dilution of both antibodies. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] - BSA and Azide free (<a href='/en-us/products/primary-antibodies/sars-cov-2-nucleocapsid-protein-antibody-epr24334-118-bsa-and-azide-free-ab280201'>ab280201</a>)

Lanes 1 and 4:

Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (<a href='/en-us/products/proteins-peptides/recombinant-human-coronavirus-sars-cov-2-nucleocapsid-protein-his-tag-ab273530'>ab273530</a>) at 0.1 µg

Lanes 2, 3 and 5:

Recombinant Human coronavirus SARS Nucleocapsid Protein (ab270829) at 0.1 µg

Lane 6:

Western blot - Recombinant Human coronavirus SARS-CoV-2 Nucleocapsid Protein (His tag) (ab272107) at 0.1 µg

Observed band size: 51 kDa

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Western blot - Recombinant Human coronavirus SARS-CoV-2 Nucleocapsid Protein (His tag) (AB272107)
  • WB

Lab

Western blot - Recombinant Human coronavirus SARS-CoV-2 Nucleocapsid Protein (His tag) (AB272107)

** Lanes 1 - 3 : ** Green - ab271180 observed at 51 kDa.** Lanes 4 - 6 : ** Red - loading control ab18184 (Mouse monoclonal [HIS.H8] to 6X His tag®) observed at 51 kDa. ab271180 was shown to react with SARS-CoV-2 nucleocapsid protein in Western blot. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab271180 and ab18184 (Mouse monoclonal [HIS.H8] to 6X His tag®) overnight at 4 °C at a 1 in 1000 dilution of both antibodies. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein (<a href='/en-us/products/primary-antibodies/rabbit-monoclonal-epr24334-118-to-sars-cov-2-nucleocapsid-protein-ab271180'>ab271180</a>) at 1/1000 dilution

Lanes 1 and 4:

Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (<a href='/en-us/products/proteins-peptides/recombinant-human-coronavirus-sars-cov-2-nucleocapsid-protein-his-tag-ab273530'>ab273530</a>) at 0.1 µg

Lanes 2 and 5:

Recombinant Human coronavirus SARS Nucleocapsid Protein (ab270829) at 0.1 µg

Lanes 3 and 6:

Western blot - Recombinant Human coronavirus SARS-CoV-2 Nucleocapsid Protein (His tag) (ab272107) at 0.1 µg

Observed band size: 51 kDa

false

SDS-PAGE - Recombinant Human coronavirus SARS-CoV-2 Nucleocapsid Protein (His tag) (AB272107)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human coronavirus SARS-CoV-2 Nucleocapsid Protein (His tag) (AB272107)

SDS-PAGE analysis of ab272107.

Key facts

Purity

>95%

Expression system

Escherichia coli

Tags

His tag C-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P59595

Animal free

No

Carrier free

No

Species

SARS-CoV

Storage buffer

pH: 7 - 8 Constituents: 99% DPBS

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Recombinant Human coronavirus SARS CoV-2 Nucleocapsid Protein (His tag) tends to aggregate in solution.
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Sequence info

[{"sequence":"MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQARNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":419,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P59595","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The nucleocapsid protein of SARS-CoV-2 also known as N protein or COVID-19 nucleocapsid protein is important for the viral replication and packaging processes. It has a mass of approximately 43-46 kDa and is expressed in the cytoplasm of infected host cells. This protein is highly conserved among coronaviruses and plays a significant role in the viral lifecycle by binding to the viral RNA genome thereby facilitating its encapsulation within the virion. Moreover it aids in stabilizing the viral RNA structure and is essential for ribonucleoprotein complex formation.
Biological function summary

The nucleocapsid protein of SARS-CoV-2 interacts directly with other viral components and host cellular machinery to orchestrate efficient viral assembly and release. It forms a ribonucleoprotein complex by self-association and binding to the RNA genome promoting virion integrity. This protein also plays a part in modulating host cell responses potentially by interfering with antiviral signaling pathways ensuring that the virus evades the host immune system to some degree.

Pathways

Many scientific studies show that the SARS-CoV-2 nucleocapsid protein is involved in the viral replication and assembly pathways. It interacts with viral proteins such as the membrane (M) protein and possibly others to facilitate these processes. The protein also exhibits connectivity with cellular pathways related to RNA processing and metabolism due to its RNA-binding capabilities further influencing viral replication efficiency and host-pathogen interactions.

The nucleocapsid protein has a significant association with COVID-19 the disease caused by SARS-CoV-2. Its expression correlates with viral pathogenicity and immune response dysregulation which can lead to severe outcomes in infected individuals. The N protein has links with comorbid conditions potentially affecting disease progression. Furthermore the nucleocapsid protein interacts with host proteins like G3BP1 and other cellular factors that may contribute to immune modulation influencing the clinical symptoms associated with COVID-19.
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Specifications

Form

Liquid

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General info

Function

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication (PubMed : 17210170). May modulate transforming growth factor-beta signaling by binding host SMAD3 (PubMed : 18055455).

Sequence similarities

Belongs to the betacoronavirus nucleocapsid protein family.

Post-translational modifications

Proteolytically cleaved by host CASP6. The cleavage leads to two fragments and facilitates viral replication by inhibiting host IFN signaling. The two fragments may interact with IRF3 inhibiting its nuclear translocation after activation and reduce the expression of IFNB and IFN-stimulated genes.. ADP-ribosylated. The ADP-ribosylation is retained in the virion during infection.. Phosphorylated on serine and threonine residues (PubMed:19106108). Phosphorylated by host GSK3A and GSK3B. Phosphorylation allows recruitment of host RNA helicase DDX1 which facilitates template readthrough and enables longer subgenomic mRNA synthesis. This promotes the solubility of homodimers that would otherwise aggregate (PubMed:32974389). Host phosphatase would dephosphorylate the protein during assembly at M bound membranes (PubMed:32974389).

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Product protocols

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Target data

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication (PubMed : 17210170). May modulate transforming growth factor-beta signaling by binding host SMAD3 (PubMed : 18055455).
See full target information N

Additional targets

SARS Nucleocapsid Protein
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 13:963627 PubMed35928816

2022

The SARS-CoV-2 Nucleoprotein Induces Innate Memory in Human Monocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Patricia Urbán,Paola Italiani,Diana Boraschi,Sabrina Gioria

Biochemical and biophysical research communications 557:273-279 PubMed33894414

2021

In-vitro acetylation of SARS-CoV and SARS-CoV-2 nucleocapsid proteins by human PCAF and GCN5.

Applications

Unspecified application

Species

Unspecified reactive species

Dai Hatakeyama,Takeshi Masuda,Ryosuke Miki,Sumio Ohtsuki,Takashi Kuzuhara
View all publications
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