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Recombinant Human coronavirus SARS-CoV-2 Nucleocapsid Protein (His tag) is a SARS-CoV Full Length protein, in the 1 to 419 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE.

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Images

Western blot - Recombinant Human coronavirus SARS-CoV-2 Nucleocapsid Protein (His tag) (AB272107), expandable thumbnail
  • SDS-PAGE - Recombinant Human coronavirus SARS-CoV-2 Nucleocapsid Protein (His tag) (AB272107), expandable thumbnail
  • Western blot - Recombinant Human coronavirus SARS-CoV-2 Nucleocapsid Protein (His tag) (AB272107), expandable thumbnail

Publications

Key facts

Purity
>95%
Expression system
Escherichia coli
Tags
His tag C-Terminus
Applications
SDS-PAGE
Biologically active
No

Amino acid sequence

M S D N G P Q N Q R N A P R I T F G G P S D S T G S N Q N G E R S G A R S K Q R R P Q G L P N N T A S W F T A L T Q H G K E D L K F P R G Q G V P I N T N S S P D D Q I G Y Y R R A T R R I R G G D G K M K D L S P R W Y F Y Y L G T G P E A G L P Y G A N K D G I I W V A T E G A L N T P K D H I G T R N P A N N A A I V L Q L P Q G T T L P K G F Y A E G S R G G S Q A S S R S S S R S R N S S R N S T P G S S R G T S P A R M A G N G G D A A L A L L L L D R L N Q L E S K M S G K G Q Q Q Q G Q T V T K K S A A E A S K K P R Q K R T A T K A Y N V T Q A F G R R G P E Q T Q G N F G D Q E L I R Q G T D Y K H W P Q I A Q F A P S A S A F F G M S R I G M E V T P S G T W L T Y T G A I K L D D K D P N F K D Q V I L L N K H I D A Y K T F P P T E P K K D K K K K A D E T Q A L P Q R Q K K Q Q T V T L L P A A D L D D F S K Q L Q Q S M S S A D S T Q A R N P A N N A A I V L Q L P Q G T T L P K G F Y A E G S R G G S Q A S S R S S S R S R N S S R N S T P G S S R G T S P A R M A G N G G D A A L A L L L L D R L N Q L E S K M S G K G Q Q Q Q G Q T V T K K S A A E A S K K P R Q K R T A T K A Y N V T Q A F G R R G P E Q T Q G N F G D Q E L I R Q G T D Y K H W P Q I A Q F A P S A S A F F G M S R I G M E V T P S G T W L T Y T G A I K L D D K D P N F K D Q V I L L N K H I D A Y K T F P P T E P K K D K K K K A D E T Q A L P Q R Q K K Q Q T V T L L P A A D L D D F S K Q L Q Q S M S S A D S T Q A

Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Target data

Function

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication (PubMed:17210170). May modulate transforming growth factor-beta signaling by binding host SMAD3 (PubMed:18055455).

Additional Targets

SARS Nucleocapsid Protein

Alternative names

9a, N, Nucleoprotein, Nucleocapsid protein, NC, Protein N

Recommended products

Recombinant Human coronavirus SARS-CoV-2 Nucleocapsid Protein (His tag) is a SARS-CoV Full Length protein, in the 1 to 419 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE.

Key facts

Purity
>95%
Expression system
Escherichia coli
Applications
SDS-PAGE
Accession
P59595-1
Animal free
No
Species
SARS-CoV
Concentration
Loading...
Storage buffer

pH: 7 - 8
Constituents: 99% DPBS

Sequence info

Amino acid sequence

M S D N G P Q N Q R N A P R I T F G G P S D S T G S N Q N G E R S G A R S K Q R R P Q G L P N N T A S W F T A L T Q H G K E D L K F P R G Q G V P I N T N S S P D D Q I G Y Y R R A T R R I R G G D G K M K D L S P R W Y F Y Y L G T G P E A G L P Y G A N K D G I I W V A T E G A L N T P K D H I G T R N P A N N A A I V L Q L P Q G T T L P K G F Y A E G S R G G S Q A S S R S S S R S R N S S R N S T P G S S R G T S P A R M A G N G G D A A L A L L L L D R L N Q L E S K M S G K G Q Q Q Q G Q T V T K K S A A E A S K K P R Q K R T A T K A Y N V T Q A F G R R G P E Q T Q G N F G D Q E L I R Q G T D Y K H W P Q I A Q F A P S A S A F F G M S R I G M E V T P S G T W L T Y T G A I K L D D K D P N F K D Q V I L L N K H I D A Y K T F P P T E P K K D K K K K A D E T Q A L P Q R Q K K Q Q T V T L L P A A D L D D F S K Q L Q Q S M S S A D S T Q A R N P A N N A A I V L Q L P Q G T T L P K G F Y A E G S R G G S Q A S S R S S S R S R N S S R N S T P G S S R G T S P A R M A G N G G D A A L A L L L L D R L N Q L E S K M S G K G Q Q Q Q G Q T V T K K S A A E A S K K P R Q K R T A T K A Y N V T Q A F G R R G P E Q T Q G N F G D Q E L I R Q G T D Y K H W P Q I A Q F A P S A S A F F G M S R I G M E V T P S G T W L T Y T G A I K L D D K D P N F K D Q V I L L N K H I D A Y K T F P P T E P K K D K K K K A D E T Q A L P Q R Q K K Q Q T V T L L P A A D L D D F S K Q L Q Q S M S S A D S T Q A
Accession
P59595
Protein length
Full Length
Amino acids
1 to 419
Nature
Recombinant
Tags
His tag C-Terminus

Specifications

Form
Liquid

General info

Function

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication (PubMed:17210170). May modulate transforming growth factor-beta signaling by binding host SMAD3 (PubMed:18055455).

Sequence similarities

Belongs to the betacoronavirus nucleocapsid protein family.

Post-translational modifications

Proteolytically cleaved by host CASP6. The cleavage leads to two fragments and facilitates viral replication by inhibiting host IFN signaling. The two fragments may interact with IRF3 inhibiting its nuclear translocation after activation and reduce the expression of IFNB and IFN-stimulated genes.

Storage

Shipped at conditions
Dry Ice
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Recombinant Human coronavirus SARS CoV-2 Nucleocapsid Protein (His tag) tends to aggregate in solution.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The nucleocapsid protein of SARS-CoV-2 also known as N protein or COVID-19 nucleocapsid protein is important for the viral replication and packaging processes. It has a mass of approximately 43-46 kDa and is expressed in the cytoplasm of infected host cells. This protein is highly conserved among coronaviruses and plays a significant role in the viral lifecycle by binding to the viral RNA genome thereby facilitating its encapsulation within the virion. Moreover it aids in stabilizing the viral RNA structure and is essential for ribonucleoprotein complex formation.

Biological function summary

The nucleocapsid protein of SARS-CoV-2 interacts directly with other viral components and host cellular machinery to orchestrate efficient viral assembly and release. It forms a ribonucleoprotein complex by self-association and binding to the RNA genome promoting virion integrity. This protein also plays a part in modulating host cell responses potentially by interfering with antiviral signaling pathways ensuring that the virus evades the host immune system to some degree.

Pathways

Many scientific studies show that the SARS-CoV-2 nucleocapsid protein is involved in the viral replication and assembly pathways. It interacts with viral proteins such as the membrane (M) protein and possibly others to facilitate these processes. The protein also exhibits connectivity with cellular pathways related to RNA processing and metabolism due to its RNA-binding capabilities further influencing viral replication efficiency and host-pathogen interactions.

Associated diseases and disorders

The nucleocapsid protein has a significant association with COVID-19 the disease caused by SARS-CoV-2. Its expression correlates with viral pathogenicity and immune response dysregulation which can lead to severe outcomes in infected individuals. The N protein has links with comorbid conditions potentially affecting disease progression. Furthermore the nucleocapsid protein interacts with host proteins like G3BP1 and other cellular factors that may contribute to immune modulation influencing the clinical symptoms associated with COVID-19.

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